EP0230472A1
Expired / Lapsed
EP
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Biosensor and method of manufacturing same
Assignee: Panasonic Holdings Corp
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Filing: 1986-06-19
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Granted: —
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Expires: 2006-06-19
Abstract
Abstract The electrode systems (13, 14, 15) consisting mainly of carbon on an insulated substrate (12), and the surfaces of said electrode systems are coated with an insulating layer (16) while parts (13', 14', 15') thereof being exposed. A porous member (18), which retains oxygen and electron receptors thereon, and a retainer frame (17) are then provided on the upper surface of the resultant product. A cover (19) having an opening with the diameter smaller than that of the porous member (18) is then placed on the resultant product, and all of these parts are combined integrally. Owing to such a construction, an inexpensive disposable biosensor capable of carrying out a measuring operation easily can be obtained. When heat-treated at 60-170°C for 1-8 hours during the formation of the electrode systems, the preservation stability of the biosensor can be improved. By subjecting the surfaces of the measuring electrodes to a protein adsorption treatment, the dispersion of response currents can be prevented.
Inventors
- 👤 Shiro Nankai
- 👤 Mariko Kawaguri
- 👤 Takashi Iijima
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 22:39
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3271; G01N27/3272; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
101 words
A biosensor of the type which comprises an insulative base having an electrode system which includes at least a measuring electrode and a counter electrode and in which a variation in concentration of a substance occurring during reactions between an oxydo-reductase, an electron acceptor and a liquid sample is electrochemically detected to measure a concentration of a substrate in said sample liquid, characterized in that said electrode system is made primarily of carbon and is covered with a perforated body having said enzyme and said electron acceptor and that said perforated body is integrally combined with said electrode.system and said base.
Claim 2
Dependent Claim
101 words
A biosensor of the type which comprises an insulative base having an electrode system which includes at least a measuring electrode and a counter electrode and in which a variation in concentration of a substance occurring during reactions between an oxydo-reductase, an electron acceptor and a liquid sample is electrochemically detected to measure a concentration of a substrate in said sample liquid, characterized in that said electrode system is made primarily of carbon and is covered with a perforated body having said enzyme and said electron acceptor and that said perforated body is integrally combined with said electrode.system and said base.
Claim 3
Dependent Claim
21 words
A biosensor according to Claim 1, wherein said electrode system includes the measuring electrode, the counter electrode and a reference electrode.
Claim 4
Dependent Claim
20 words
A biosensor according to Claim 1, wherein said electrode system is formed by application or printing of a carbon paste.
Claim 5
Dependent Claim
20 words
A biosensor according to Claim 1, wherein the surface of at least the measuring electrode is covered with a protein.
Claim 6
Dependent Claim
14 words
A biosensor according to Claim 4, wherein said protein is alubmin or glucose oxidase.
Claim 7
Dependent Claim
117 words
A biosensor of the type which comprises an insulative base having an electrode system which includes at least a measuring electrode and a counter electrode and in which a variation in concentration of a substance occurring during reactions between an oxydo-reductase, an electron acceptor and a liquid sample is electrochemically detected to measure a concentration of a substrate in said sample liquid, characterized in that said electrode system is made primarily of carbon and is covered with a perforated body having said enzyme and said electron acceptor, a sample addition layer and a liquid-retaining layer superposed in this order and that said perforated body, said sample-accepting layer and said liquid-retaining layer are integrally combined with said base.
Claim 8
Dependent Claim
117 words
A biosensor of the type which comprises an insulative base having an electrode system which includes at least a measuring electrode and a counter electrode and in which a variation in concentration of a substance occurring during reactions between an oxydo-reductase, an electron acceptor and a liquid sample is electrochemically detected to measure a concentration of a substrate in said sample liquid, characterized in that said electrode system is made primarily of carbon and is covered with a perforated body having said enzyme and said electron acceptor, a sample addition layer and a liquid-retaining layer superposed in this order and that said perforated body, said sample-accepting layer and said liquid-retaining layer are integrally combined with said base.
Claim 9
Dependent Claim
20 words
A biosensor according to Claim 6, wherein a filtration layer is provided on the liquid-retaining layer covering said electrode system.
Claim 10
Dependent Claim
23 words
A biosensor according to Claim 6, wherein the surface of said electrode system made of a carbon paste is covered with a protein.
Claim 11
Dependent Claim
102 words
A biosensor according to Claim 6, wherein the electrode system mounted on said insulative base and primarily made of carbon is covered with the liquid-retaining layer whose height is regulated with two resin plates; a filtration layer, the perforated body and the sample addition layer which are held with a resin holding frame are placed on the liquid-retaining layer, and said holding frame is fixed to said base; and a resin cover is provided on the upper surface of said holding frame and has an opening whose diameter is smaller than outer diameters of said perforated body and said sample addition layer.
Claim 12
Independent Claim
97 words
A method for making a biosensor characterized by comprising providing an insulative base, printing or applying a carbon paste on said base to form an electrode system including, at least, a measuring electrode and a counter electrode, polishing the surface of the respective electrodes and subjecting the polished electrodes to a thermal treatment at a temperature of 60 - 170 0 C for 1 - 8 hours, covering the electrode system with a perforated body having an oxydo-reductase and an electron acceptor therein, and integrally combining said perforated body with said electrode system and said insulative base.
Claim 13
Independent Claim
97 words
A method for making a biosensor characterized by comprising providing an insulative base, printing or applying a carbon paste on said base to form an electrode system including, at least, a measuring electrode and a counter electrode, polishing the surface of the respective electrodes and subjecting the polished electrodes to a thermal treatment at a temperature of 60 - 170 0 C for 1 - 8 hours, covering the electrode system with a perforated body having an oxydo-reductase and an electron acceptor therein, and integrally combining said perforated body with said electrode system and said insulative base.
Claim 14
Dependent Claim
28 words
A method for making a biosensor according to Claim 10, wherein the thermal treatment is effected at a temperature of 70 - 150 0 C for 4 hours.
References Cited (Backward Citations)
No citation records indexed.
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