EP0229750A2
Expired / Lapsed
EP
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Human preproinsulin-like growth factor I
Assignee: University of Washington
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Filing: 1987-01-06
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Granted: —
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Expires: 2007-01-06
Abstract
Abstract The invention provides DNA sequences encoding a human preproinsulin-like growth factor I protein and novel extension peptides. A novel preproinsulin-like growth factor I protein and novel extension peptides are also provided. The present invention further provides a human IGF-I gene which has been sequenced and which encodes at least two preproinsulin-like growth factor-I proteins. Various genes and DNA sequences useful in producing essentially pure mature IGF-1, preproinsulin-like growth factor I proteins and IGF-I gene related proteins are also provided.
Inventors
- 👤 Gwen Grabowski Krivi
- 👤 Peter Scott Rotwein
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 13:55
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/65; A; A61; A61P; A61P15/00; A61P15/14; A61P43/00
Claims Analysis
Total Claims: 46
Claim 1
Independent Claim
20 words
A synthetic DNA sequence encoding a preproinsulin-like growth factor-I protein comprising the sequence of amino acids shown in Figure 2.
Claim 2
Dependent Claim
20 words
A synthetic DNA sequence encoding a preproinsulin-like growth factor-I protein comprising the sequence of amino acids shown in Figure 2.
Claim 3
Dependent Claim
21 words
A synthetic DNA sequence comprising the sequence of nucleotides from about nucleotide 1 to about nucleotide 1136 shown in Figure 2.
Claim 4
Dependent Claim
21 words
A synthetic DNA sequence comprising the sequence of nucleotides from about nucleotide 1 to about nucleotide 1136 shown in Figure 2.
Claim 5
Dependent Claim
21 words
A synthetic DNA sequence comprising the sequence of nucleotides from about nucleotide 183 to about nucleotide 767 shown in Figure 2.
Claim 6
Dependent Claim
21 words
A synthetic DNA sequence comprising the sequence of nucleotides from about nucleotide 183 to about nucleotide 767 shown in Figure 2.
Claim 7
Dependent Claim
28 words
A synthetic DNA sequence encoding a mature human IGF-I peptide linked directly at its carboxy-end to the amino terminus a peptide comprising the following sequence of amino acids:
Claim 8
Dependent Claim
28 words
A synthetic DNA sequence encoding a mature human IGF-I peptide linked directly at its carboxy-end to the amino terminus a peptide comprising the following sequence of amino acids:
Claim 9
Dependent Claim
23 words
A DNA sequence of Claim 4 comprising the sequence of nucleotides from about nucleotide 327 to about nucleotide 767 shown in Figure 2.
Claim 10
Dependent Claim
15 words
A synthetic DNA sequence encoding a peptide comprising of the following sequence of amino acids:
Claim 11
Dependent Claim
15 words
A synthetic DNA sequence encoding a peptide comprising of the following sequence of amino acids:
Claim 12
Dependent Claim
23 words
A DNA sequence of Claim 6 comprising the sequence of nucleotides from about nucleotide 537 to about nucleotide 767 shown in Figure 2.
Claim 13
Dependent Claim
15 words
A synthetic DNA sequence encoding a peptide comprising of the following sequence of amino acids:
Claim 14
Dependent Claim
15 words
A synthetic DNA sequence encoding a peptide comprising of the following sequence of amino acids:
Claim 15
Dependent Claim
12 words
A DNA sequence of Claim 8 comprising the following sequence of nucleotides:
Claim 16
Dependent Claim
20 words
An essentially intron-free DNA sequence encoding a preproinsulin-like growth factor-I comprising the sequence of amino acids shown in Figure 6.
Claim 17
Dependent Claim
20 words
An essentially intron-free DNA sequence encoding a preproinsulin-like growth factor-I comprising the sequence of amino acids shown in Figure 6.
Claim 18
Dependent Claim
17 words
An essentially pure preproinsulin-like growth factor-I protein comprising the sequence of amino acids shown in Figure 6.
Claim 19
Dependent Claim
17 words
An essentially pure preproinsulin-like growth factor-I protein comprising the sequence of amino acids shown in Figure 6.
Claim 20
Dependent Claim
27 words
An essentially pure mature human insulin-like growth factor-I peptide linked directly at its carboxy-end to the amino-terminus of a peptide comprising the following sequence of amino acids:
Claim 21
Dependent Claim
27 words
An essentially pure mature human insulin-like growth factor-I peptide linked directly at its carboxy-end to the amino-terminus of a peptide comprising the following sequence of amino acids:
Claim 24
Dependent Claim
12 words
An essentially pure extension peptide comprising the following sequence of amino acids:
Claim 25
Dependent Claim
12 words
An essentially pure extension peptide comprising the following sequence of amino acids:
Claim 26
Dependent Claim
12 words
An essentially pure extension peptide comprising the following sequence of amino acids:
Claim 27
Dependent Claim
12 words
An essentially pure extension peptide comprising the following sequence of amino acids:
Claim 28
Dependent Claim
11 words
An essentially intron-free DNA sequence encoding the peptide of Claim 12.
Claim 29
Dependent Claim
12 words
An essentially intron-free DNA sequence encoding the extension peptide of Claim 13.
Claim 30
Dependent Claim
12 words
An essentially intron-free DNA sequence encoding the extension peptide of Claim 14.
Claim 31
Dependent Claim
14 words
A recombinant DNA vector containing a DNA sequence encoding the protein of Claim 11.
Claim 32
Dependent Claim
33 words
A recombinant DNA vector containing a DNA sequence encoding a protein selected from the group consisting of the protein of Claim 12, the protein of Claim 13 and the protein of Claim 14.
Claim 33
Dependent Claim
34 words
An essentially pure protein encoded in a human messenger RNA molecule which RNA molecular hybridizes to a DNA sequence encoding a preproinsulin-like growth factor-I protein comprising the amino acid sequence shown in Figure 6.
Claim 34
Dependent Claim
34 words
An essentially pure protein encoded in a human messenger RNA molecule which RNA molecular hybridizes to a DNA sequence encoding a preproinsulin-like growth factor-I protein comprising the amino acid sequence shown in Figure 6.
Claim 35
Dependent Claim
31 words
A protein of Claim 20 in which the human messenger RNA molecule is selected from the group consisting of a RNA molecule 1.7 kb, 3.7 kb and 6.3 kb in length.
Claim 36
Dependent Claim
95 words
An essentially pure protein encoded in a human messenger RNA molecule which messenger RNA molecule hybridizes to a DNA sequence encoding a peptide comprising the following amino acid sequence: NH 2 - Tyr Gln Pro Pro Ser Thr Asn Lys Asn Thr Lys Ser Gln Arg Arg Lys Gly Trp Pro Lys Thr His Pro Gly Gly Glu Gln Lys Glu Gly Thr Glu Ala Ser Leu Gln Ile Arg Gly Lys Lys Lys Glu Gln Arg Arg Glu Ile Gly Ser Arg Asn Ala Glu Cys Arg Gly Lys Lys Gly Lys - COOH.
Claim 37
Dependent Claim
95 words
An essentially pure protein encoded in a human messenger RNA molecule which messenger RNA molecule hybridizes to a DNA sequence encoding a peptide comprising the following amino acid sequence: NH 2 - Tyr Gln Pro Pro Ser Thr Asn Lys Asn Thr Lys Ser Gln Arg Arg Lys Gly Trp Pro Lys Thr His Pro Gly Gly Glu Gln Lys Glu Gly Thr Glu Ala Ser Leu Gln Ile Arg Gly Lys Lys Lys Glu Gln Arg Arg Glu Ile Gly Ser Arg Asn Ala Glu Cys Arg Gly Lys Lys Gly Lys - COOH.
Claim 38
Dependent Claim
30 words
A rotein of Claim 22 in which the human messenger RNA molecule is selected from the group consisting of a RNAmolecule 1.7 kb, 3.7 kb and 6.3 kb in length.
Claim 39
Dependent Claim
14 words
An essentially pure human IGF-I gene encoding at least two preproinsulin-like growth factor-I proteins.
Claim 40
Dependent Claim
14 words
An essentially pure human IGF-I gene encoding at least two preproinsulin-like growth factor-I proteins.
Claim 41
Dependent Claim
22 words
The gene of Claim 24 comprising the sequence of nucleotides from about nucleotide 1 to about nucleotide 4349 shown in Figure 4.
Claim 42
Dependent Claim
21 words
The gene of Claim 24 encoding a preproinsulin-like growth factor-I protein comprising the sequence of amino acids shown in Figure 6.
Claim 43
Dependent Claim
25 words
A method for promoting growth in an animal comprising administering to the animal a therapeutically effective amount of a protein as defined in Claim 11.
Claim 44
Dependent Claim
25 words
A method for enhancing lactation in a cow comprising administering to the cow a therapeutically effective amount of a protein as defined in Claim 11.
Claim 45
Dependent Claim
24 words
A therapeutic composition for promoting growth comprising a therapeutically effective amount of the protein of Claim 11 and a pharmaceutically acceptable carrier or diluent.
Claim 46
Dependent Claim
27 words
A therapeutic composition for enhancing lactation in a cow comprising a therapeutically effective amount of the protein of Claim 11 and a pharmaceutically acceptable carrier or diluent.
Claim 47
Dependent Claim
20 words
An essentially pure DNA molecule comprising the sequence of nucleotides in the IGF-I gene exon 4 shown in Figure 4.
Claim 48
Dependent Claim
20 words
An essentially pure DNA molecule comprising the sequence of nucleotides in the IGF-I gene exon 4 shown in Figure 4.
References Cited (Backward Citations)
No citation records indexed.
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