EP0227169A2
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Immunochemical reagent
Assignee: Akzo NV
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Filing: 1986-12-08
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Granted: —
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Expires: 2006-12-08
Abstract
Abstract The present invention is concerned with an immunochemical reagent that comprises a combination of two or more of the synthetic polypeptide sequences mimicking at least one antigenic determinant of the gag -antigen, gp120 and gp41 of HTLV-III. This immunochemical reagent is particularly useful in immunoassays and for immunization.
Inventors
- 👤 Michael L. Berman
- 👤 Sylvia A. Crush
- 👤 Flossie Wong-Staal
- 👤 Robert C. Gallo
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 01:15
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61P; A61P31/00; A61P31/12; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/72; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6878; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16111; C12N2740/16122
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
71 words
Immunochemical reagent characterized in that it comprises a combination of two or more synthetic polypeptide sequences selected from the group consisting of: - a polypeptide sequence mimicking at least one antigenic determinant of the gag -antigen of HTLV-III; - a polypeptide sequence mimicking at least one antigenic determinant of the glycoprotein gp120 of HTLV-III; and - a polypeptide sequence mimicking at least one antigenic determinant of the glycoprotein gp41 of HTLV-III.
Claim 2
Dependent Claim
71 words
Immunochemical reagent characterized in that it comprises a combination of two or more synthetic polypeptide sequences selected from the group consisting of: - a polypeptide sequence mimicking at least one antigenic determinant of the gag -antigen of HTLV-III; - a polypeptide sequence mimicking at least one antigenic determinant of the glycoprotein gp120 of HTLV-III; and - a polypeptide sequence mimicking at least one antigenic determinant of the glycoprotein gp41 of HTLV-III.
Claim 3
Dependent Claim
31 words
Immunochemical reagent according to claim 1, characterized in that it comprises a polypeptide having the same immunochemical properties as the carboxyterminal about 58 amino acids long polypeptide of the gp120 protein.
Claim 4
Dependent Claim
28 words
Immunochemical reagent according to claim 2, characterized in that it comprises a polypeptide having the same immunochemical properties as a polypeptide of the sequence represented in figure 9A.
Claim 5
Dependent Claim
31 words
Immunochemical reagent according to claim 1, characterized in that it comprises a polypeptide having the same immunochemical properties as the carboxyterminal about 38 amino acids long polypeptide of the gp120 protein.
Claim 6
Dependent Claim
30 words
Immunochemical reagent according to claim 4, characterized in that it comprises a polypeptide having the same immunochemical properties as any of the polypeptides of the sequences represented in figure 9B.
Claim 7
Dependent Claim
41 words
Immunochemical reagent according to claim 1-5, characterized in that it comprises a polypeptide having the same immunochemical properties as the partial amino acid sequence extending between about amino acid no. 53 and about amino acid no. 122 of the gp41 protein.
Claim 8
Dependent Claim
28 words
Immunochemical reagent according to claim 6, characterized in that it comprises a polypeptide having the same immunochemical properties as a polypeptide of the sequences represented in figure 10.
Claim 9
Dependent Claim
41 words
Immunochemical reagent according to claim 1-7, characterized in that it comprises a polypeptide having the same immunochemical properties as the partial amino acid sequence extending between about amino acid no. 123 and about amino acid no. 172 of the gp41 protein.
Claim 10
Dependent Claim
28 words
Immunochemical reagent according to claim 8, characterized in that it comprises a polypeptide having the same immunochemical properties as a polypeptide of the sequences represented in figure 11A.
Claim 11
Dependent Claim
41 words
Immunochemical reagent according to claim 1-7, characterized in that it comprises a polypeptide having the same immunochemical properties as the partial amino acid sequence extending between about amino acid no. 123 and about amino acid no. 163 of the gp41 protein.
Claim 12
Dependent Claim
28 words
Immunochemical reagent according to claim 10, characterized in that it comprises a polypeptide having the same immunochemical properties as a polypeptide of the sequences represented in figure 11B.
Claim 13
Dependent Claim
41 words
Immunochemical reagent according to claim 1-11, characterized in that it comprises a polypeptide having the same immunochemical properties as the partial amino acid sequences extending between about amino acid no. 173 and about amino acid no. 217 of the gp41 protein.
Claim 14
Dependent Claim
28 words
Immunochemical reagent according to claim 12, characterized in that it comprises a polypeptide having the same immunochemical properties as a polypeptide of the sequences represented in figure 12A.
Claim 15
Dependent Claim
41 words
Immunochemical reagent according to claim 1-11, characterized in that it comprises a polypeptide having the same immunochemical properties as the partial amino acid sequence extending between about amino acid no. 196 and about amino acid no. 217 of the gp41 protein.
Claim 16
Dependent Claim
28 words
Immunochemical reagent according to claim 14, characterized in that it comprises a polypeptide having the same immunochemical properties as a polypeptide of the sequences represented in figures 12B.
Claim 17
Dependent Claim
41 words
Immunochemical reagent according to claim 1-15, characterized in that it comprises a polypeptide having the same immunochemical properties as the partial amino acid sequence extending between about amino acid no. 30 and about amino acid no. 181 of the gp120 protein.
Claim 18
Dependent Claim
28 words
Immunochemical reagent according to claim 16, characterized in that it comprises a polypeptide having the same immunochemical properties as a polypeptide of the sequences represented in figure 8.
Claim 19
Dependent Claim
132 words
Synthetic polypeptide comprising at least one amino acid sequence having the immunochemical properties of the amino acid sequence of HTLV-III selected from the group consisting of: - the carboxyterminal about 38 amino acids long polypeptide of the gp120 protein; - the partial amino acid sequence extending between about amino acid no. 53 and about amino acid no. 122 of the gp41 protein; - the partial amino acid sequence extending between about amino acid no. 123 and about amino acid no. 163 of the gp41 protein; - the partial amino acid sequence extending between about amino acid no. 196 and about amino acid no. 217 of the gp41 protein; and - the partial amino acid sequence extending between about amino acid no. 30 and about amino acid no. 181 of the gp120 protein.
Claim 20
Dependent Claim
132 words
Synthetic polypeptide comprising at least one amino acid sequence having the immunochemical properties of the amino acid sequence of HTLV-III selected from the group consisting of: - the carboxyterminal about 38 amino acids long polypeptide of the gp120 protein; - the partial amino acid sequence extending between about amino acid no. 53 and about amino acid no. 122 of the gp41 protein; - the partial amino acid sequence extending between about amino acid no. 123 and about amino acid no. 163 of the gp41 protein; - the partial amino acid sequence extending between about amino acid no. 196 and about amino acid no. 217 of the gp41 protein; and - the partial amino acid sequence extending between about amino acid no. 30 and about amino acid no. 181 of the gp120 protein.
Claim 21
Dependent Claim
28 words
Synthetic polypeptide according to claim 18 comprising at least one of the amino acid sequences represented in the figures 8, 9A, 9B, 10, 11A, 11B, 12A and 12B.
Claim 22
Dependent Claim
24 words
A recombinant DNA characterized in that it comprises a DNA sequence coding for the production of a polypeptide according to claim 18 or 19.
Claim 23
Dependent Claim
14 words
A plasmid characterized in that it comprises a recombinant DNA according to claim 20.
Claim 24
Dependent Claim
16 words
A host cell characterized in that it is transformed by the plasmid according to claim 21.
Claim 25
Dependent Claim
4 words
The HTLV-III isolate λHAT-3.
Claim 26
Dependent Claim
4 words
The HTLV-III isolate λHAT-3.
Claim 27
Dependent Claim
19 words
A plasmid according to claim 21, characterized in that it is derived from the HTLV-III isolate of claim 23.
Claim 28
Dependent Claim
18 words
The plasmid of claim 24, selected from the group consisting of p24-10SB.3, pENVKPN45, pGAG8, p2-10SB.5, p28-23EB.4, and p28-EB.1.
Claim 29
Dependent Claim
36 words
The plasmid of claim 24, selected from the group consisting of pENVPSTl, pENVPSTH3, pENVPSTH3 1-2, pENVPSTH3 2-10, p2-10 EB.3, pENVKPN, pENVKPN24, pENVKPNH3 24, pENVKPNH3 24-10, pENVKPN 28, pENVKPNH3 28, pENVKPNH3 28-23, pENVKPNH3 28-24, and pENVKPNH3 28-45.
Claim 30
Dependent Claim
126 words
A method for preparing the plasmid of claim 21, comprising: (a) inserting a cDNA sequence from HTLV-III into a plasmid for transforming a cellular host (b) deleting from the plasmid of step (a) a portion of an identifiable, expressable sequence to effect an out-of-phase frameshift for that sequence; (c) transforming a a cellular host with the plasmid of step (b); (d) culturing the transformed host; (e) selecting the host cells showing spontaneous mutations that effect an in-phase frameshift for the identifiable sequence of step (b); (f) culturing the host cell selected in step (e); (g) selecting the host cell that produce useful amounts of polypeptides that are effectively immunoreactive with HTLV-III antibodies; and (h) isolating and identifying plasmids that code for the polypeptides of step (g).
Claim 31
Dependent Claim
21 words
A polypeptide characterized in that it is expressed by host cell transformed with a plasmid according to claim 25 or 26.
Claim 32
Dependent Claim
19 words
A vaccin for immunization against HTLV-III infection, characterized in that it comprises an immunochemical reagent according to claim 1-17.
Claim 33
Dependent Claim
20 words
A test kit for use in an immunoassay characterized in that it comprises an immunochemical reagent according to claim 1-17.
References Cited (Backward Citations)
No citation records indexed.
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