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EP0226979A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Process for producing the protein of an aequorin activity by using a photoprotein aequorin gene

Assignee: JNC Corp
•
Filing: 1986-12-12
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Granted: —
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Expires: 2006-12-12
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Abstract

Abstract An efficient biosynthetic process of a natural type or confluent type photoprotein aequorin is provided, which process comprises using a bacterial body transformed by inserting a substantially isolated gene pAQ 440 specifying the biosynthesis of jelly fish photoprotein aequorin into a promoter-containing plasmid.

Inventors

  • 👤 Yoshiyuki Sakaki
  • 👤 Satoshi Inouye

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-05 08:19
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43595

Claims Analysis

Total Claims: 4
Claim 1
Independent Claim 38 words
A process for producing a protein of an aequorin activity which process comprises using a bacterial body transformed by inserting a substantially isolated gene pAQ 440 specifying the biosynthesis of jelly fish photoprotein aequorin into a promoter-containing plasmid.
Claim 2
Dependent Claim 38 words
A process for producing a protein of an aequorin activity which process comprises using a bacterial body transformed by inserting a substantially isolated gene pAQ 440 specifying the biosynthesis of jelly fish photoprotein aequorin into a promoter-containing plasmid.
Claim 3
Dependent Claim 38 words
A process for producing a protein of an aequorin activity according to Claim 1 wherein lac, tac or tro originated from Escherichia coli is used as said promoter to obtain a confluent type of natural type aequorin protein.
Claim 4
Dependent Claim 35 words
A process for producing a protein of an aequorin activity according to Claim 1 wherein PL promoter of λ phage is used as said promoter to obtain a confluent type or natural type aequorin protein.

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