EP0218571A2
Expired / Lapsed
EP
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Glyphosate-resistant plants
Assignee: Monsanto Technology LLC
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Filing: 1986-08-06
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Granted: —
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Expires: 2006-08-06
Abstract
Abstract This invention involves a cloning or expression vector comprising a gene which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide which, when expressed in a plant cell contains a chloroplast transit peptide which allows the polypeptide, or an enzymatically active portion thereof, to be transported from the cytoplasm of the plant cell into a chloroplast in the plant cell, and confers a substantial degree of glyphosate resistance upon the plant cell and plants regenerated therefrom. The EPSPS coding sequence may be ligated to a strong promoter, such as the 35S promoter from cauliflower mosaic virus, to create a chimeric gene. Such genes can be inserted into plant transformation vectors, and subsequently introduced into plant cells. Plant cells transformed using such genes and plants regenerated therefrom have been shown to exhibit a substantial degree of glyphosate resistance.
Inventors
- 👤 Dilip Maganlal Shah
- 👤 Robert Bruce Horsch
- 👤 Stephen Gary Rogers
- 👤 Robert Thomas Fraley
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 12:21
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8274; C12N15/8275
Claims Analysis
Total Claims: 44
Claim 1
Independent Claim
57 words
A cloning or expression vector comprising a gene which encodes a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide under the control of a suitable promoter, said polypeptide comprising a chloroplast transit peptide which permits the polypeptide, upon expression in a plant cell, to be imported into a chloroplast of the cell, said polypeptide rendering the plant cell resistant to glyphosate.
Claim 2
Dependent Claim
57 words
A cloning or expression vector comprising a gene which encodes a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide under the control of a suitable promoter, said polypeptide comprising a chloroplast transit peptide which permits the polypeptide, upon expression in a plant cell, to be imported into a chloroplast of the cell, said polypeptide rendering the plant cell resistant to glyphosate.
Claim 3
Dependent Claim
25 words
A cloning or expression vector of Claim 1 in which the chloroplast transit peptide is derived from an EPSPS gene contained in a plant chromosome.
Claim 4
Dependent Claim
14 words
A cloning or expression vector of Claim 1 which encodes a plant EPSPS polypeptide.
Claim 5
Dependent Claim
22 words
A cloning or expression vector of Claim 1 in which the chloroplast transit peptide has substantially the sequence shown in Figure 3.
Claim 6
Dependent Claim
33 words
A cloning or expression vector of Claim l in which the promoter is heterologous with respect to the EPSPS polypeptide, said promoter causing greater expression in plant cells than the endogenous EPSPS promotor.
Claim 7
Dependent Claim
21 words
A cloning or expression vector of Claim 5 in which the heterologous promoter is derived from the genome of a virus.
Claim 8
Dependent Claim
21 words
A cloning or expression vector of Claim 6 in which the heterologous promotor is derived from a cauliflower mosaic virus (CaMV).
Claim 9
Dependent Claim
18 words
A cloning or expression vector of Claim 7 in which the heterologous promotor is the CAMV 35S promoter.
Claim 10
Dependent Claim
64 words
A plant transformation vector which comprises a) an EPSPS gene which encodes an EPSPS polypeptide under the control of a suitable promoter, said polypeptide comprising a chloroplast transit peptide which permits the polypeptide, upon expression in a plant cell, to be imported into a chloroplast of the cell, said polypeptide rendering the plant cell resistant to glyphosate; and b) at least one T-DNA border.
Claim 11
Dependent Claim
64 words
A plant transformation vector which comprises a) an EPSPS gene which encodes an EPSPS polypeptide under the control of a suitable promoter, said polypeptide comprising a chloroplast transit peptide which permits the polypeptide, upon expression in a plant cell, to be imported into a chloroplast of the cell, said polypeptide rendering the plant cell resistant to glyphosate; and b) at least one T-DNA border.
Claim 12
Dependent Claim
29 words
A plant transformation vector of Claim 9 in which the EPSPS gene comprises a CaMV 35S promotor and an EPSPS polypeptide coding sequence derived from a plant EPSPS gene.
Claim 13
Dependent Claim
17 words
A plant transformation vector of Claim 10 contained in a culture of cells having ATCC number 53213.
Claim 14
Dependent Claim
63 words
A chimeric gene which comprises: a) a coding sequence which encodes an EPSPS polypeptide, said polypeptide comprising a chloroplast transit peptide which permits the polypeptide, upon expression in a plant cell, to be imported into a chloroplast of the cell, said polypeptide rendering the plant cell resistant to glyphosate; and b) a promotor sequence which is heterologous with respect to the coding sequence.
Claim 15
Dependent Claim
63 words
A chimeric gene which comprises: a) a coding sequence which encodes an EPSPS polypeptide, said polypeptide comprising a chloroplast transit peptide which permits the polypeptide, upon expression in a plant cell, to be imported into a chloroplast of the cell, said polypeptide rendering the plant cell resistant to glyphosate; and b) a promotor sequence which is heterologous with respect to the coding sequence.
Claim 16
Dependent Claim
20 words
A chimeric gene of Claim 12 in which the chloroplast transit peptide has substantially the sequence shown in Figure 3.
Claim 17
Dependent Claim
19 words
A chimeric gene of Claim 13 in which the promotor sequence is derived from the genome of a virus.
Claim 18
Dependent Claim
18 words
A chimeric gene of Claim 14 in which the promotor sequence is derived from cauliflower mosaic virus (CaMV).
Claim 19
Dependent Claim
15 words
A chimeric gene of Claim 15 in which the promotor is the CaMV 35S promotor.
Claim 20
Dependent Claim
15 words
A plant cell which has been transformed by a plant transformation vector of Claim 9.
Claim 21
Dependent Claim
32 words
A plant cell of Claim 17 in which the plant transformation vector contains an EPSPS gene comprising a CaMV 35S promotor and an EPSPS polypeptide coding sequence from a plant EPSPS gene.
Claim 22
Dependent Claim
17 words
A plant cell of Claim 18 which is resistant to about 0.5mM glyphosate and herbicidal salts thereof.
Claim 23
Dependent Claim
11 words
A plant cell which expresses a chimeric gene of Claim 12.
Claim 24
Dependent Claim
17 words
A plant cell of Claim 20 which is resistant to about 0.5mM glyphosate and herbicidal salts thereof.
Claim 25
Dependent Claim
39 words
A method of producing glyphosate resistant plants which comprises: a) transforming plant cells by inserting into the genome of a plant cell a chimeric gene of Claim 12; and b) Regenerating glyphosate resistant plants from said transformed plant cells.
Claim 26
Dependent Claim
4 words
A glyphosate-resistant tomato plant.
Claim 27
Dependent Claim
4 words
A glyphosate-resistant tomato plant.
Claim 28
Dependent Claim
4 words
A glyphosate-resistant tobacco plant.
Claim 29
Dependent Claim
4 words
A glyphosate-resistant tobacco plant.
Claim 30
Dependent Claim
5 words
A glyphosate-resistant oil seed rape.
Claim 31
Dependent Claim
5 words
A glyphosate-resistant oil seed rape.
Claim 32
Dependent Claim
4 words
A glyphosate-resistant flax plant.
Claim 33
Dependent Claim
4 words
A glyphosate-resistant flax plant.
Claim 34
Dependent Claim
4 words
A glyphosate-resistant soybean plant.
Claim 35
Dependent Claim
4 words
A glyphosate-resistant soybean plant.
Claim 36
Dependent Claim
4 words
A glyphosate-resistant sunflower plant.
Claim 37
Dependent Claim
4 words
A glyphosate-resistant sunflower plant.
Claim 38
Dependent Claim
5 words
A glyphosate-resistant sugar beet plant.
Claim 39
Dependent Claim
5 words
A glyphosate-resistant sugar beet plant.
Claim 40
Dependent Claim
4 words
A glyphosate-resistant alfalfa plant.
Claim 41
Dependent Claim
4 words
A glyphosate-resistant alfalfa plant.
Claim 42
Independent Claim
33 words
A method for isolating EPSPS genes which comprises hybridizing the EPSPS gene from a first plant species with DNA containing the EPSPS coding sequence of a second plant species or a fragment thereof.
Claim 43
Independent Claim
33 words
A method for isolating EPSPS genes which comprises hybridizing the EPSPS gene from a first plant species with DNA containing the EPSPS coding sequence of a second plant species or a fragment thereof.
Claim 44
Dependent Claim
13 words
A method of Claim 31 in which the second plant species is petunia.
References Cited (Backward Citations)
No citation records indexed.
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