Epidermal cell extracts and method to enhance wound healing and regenerate epidermis

Claim 2

The use of an aqueous, cell-free extract derived from cultured human epidermal cells for the manufacture of a composition useful for stimulating the growth or regeneration of epidermal cells in a subject whose epidermal cells are not growing or regenerating adequately.

Claim 3

The use of claim 2, wherein the subject's epidermal cells are contacted with an adsorbing material and/or a matrix carrying the cell-free extract derived from epidermal cells.

Claim 4

The use of claim 2, wherein the subject is a mammal.

Claim 5

The use of claim 4, wherein the mammal is a human or swine.

Claim 6

The use of an aqueous, cell-free extract from cultured human epidermal cells for the manufacture of a composition useful for enhancing wound coverage and healing in a wounded subject.

Claim 7

The use of an aqueous, cell-free extract from cultured human epidermal cells for the manufacture of a composition useful for enhancing the wound coverage of transplanted skin material situated on a wounded region of a subject.

Claim 8

The use of claim 7, wherein the transplanted skin material is autologous relative to the subject.

Claim 9

The use of claim 7, wherein the transplanted skin material is allogeneic relative to the subject.

Claim 10

The use of claim 7, wherein the transplanted skin material is tissue culture grown epidermal cells.

Claim 11

A composition capable of stimulating epidermal cell growth, derived from an aqueous, cell-free extract of claim 1.

Claim 12

A pharmaceutical composition comprising an amount of the aqueous, cell-free extract of claim 1 or or 11, effective to stimulate growth and regeneration of epidermal cells and a pharmaceutically acceptable carrier.

Claim 13

A wound dressing comprising an effective wound healing amount of the composition of claim 1 or 11 and an adsorbing material and/or a matrix carrying the composition.