EP0210628A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Antigenic modification of polypeptides
Assignee: The Ohio State University
•
Filing: 1983-05-20
•
Granted: —
•
Expires: 2003-05-20
Abstract
Abstract Modified polypeptides capable of provoking the formation of antibodies in an animal may be produced by forming a linear polymer of polypeptide fragments, each having a molecular structure similar to a fragment of the protein to which antibodies are to be provoked. Such linear polymers can be made more immunogenic than the proteins to which they are related without introducing undesirable extraneous materials into the animal being treated, but have reproduc- able immunogenic properties. Proteins which are not endogenous or immunogenic to an animal can be chemically modified so as to make them more immunogenic. Also, modified antigens useful for fertility control can be produced by chemi- cal modification of zona pellucida or sperm antigens. These modified polypeptides, antigens and modified antigens are desirably administered in the form of a vaccine having a vehicle comprising a mixture of mannide monooleate with Squalene and/or Squalene.
Inventors
- 👤 Vernon Cécil Stevens
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 11:26
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/107; C07K1/1072; C07K1/1075; A; A61; A61K; A61K39/00; A61K39/0005; A61K39/0006; A61K39/385; C07K1/1077; C07K14/00; C07K14/435; C07K14/575; C07K14/59; A61K2039/555; A61K2039/55511; A61K2039/5555; A61K2039/55555; A61K2039/55566; A61K2039/60; A61K2039/6012; A61K2039/62; A61K2039/627; A61K2039/64; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S930/00; Y10S930/01; Y10S930/11
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
167 words
A process for preparing an antigen for provoking the formation, in the body of an animal, of antibodies to a protein which is not endogenous or substantially immunogenic to the animal, the process being characterized by: procuring the protein, or a peptide having a sequence corresponding to at least part of the sequence of the protein, the protein or peptide having a sulfhydryl group thereon; treating the protein or peptide with an activator of the structure wherein X represents a non-reacting group comprising substituted or unsubstituted phenyl or C l-19 alkylene moieties, or a combination thereof, or an amino acid chain, so as to cause reaction of the maleiimide group of the activator with the sulfhydryl group on the protein or peptide; and treating the resulting activated protein or peptide at slightly alkaline pH with a carrier moiety biologically foreign to the animal and selected having a size sufficient to elicit antibody response following the administration thereof into the body of the animal, thereby producing the antigen.
Claim 2
Dependent Claim
167 words
A process for preparing an antigen for provoking the formation, in the body of an animal, of antibodies to a protein which is not endogenous or substantially immunogenic to the animal, the process being characterized by: procuring the protein, or a peptide having a sequence corresponding to at least part of the sequence of the protein, the protein or peptide having a sulfhydryl group thereon; treating the protein or peptide with an activator of the structure wherein X represents a non-reacting group comprising substituted or unsubstituted phenyl or C l-19 alkylene moieties, or a combination thereof, or an amino acid chain, so as to cause reaction of the maleiimide group of the activator with the sulfhydryl group on the protein or peptide; and treating the resulting activated protein or peptide at slightly alkaline pH with a carrier moiety biologically foreign to the animal and selected having a size sufficient to elicit antibody response following the administration thereof into the body of the animal, thereby producing the antigen.
Claim 3
Dependent Claim
186 words
A process for preparing an antigen for provoking the formation, in the body of an animal, of antibodies to a protein which is not endogenous or substantially immunogenic to the animal, the process being characterized by: procuring a carrier which is biologically foreign to the animal, has a size sufficient to elicit antibody response following the administration thereof into the body of the animal and does not have a sulfhydryl group but has an amino group; treating the carrier under neutral or acid conditions with an activator of the structure wherein X represents a non-reacting group comprising substituted or unsubstituted phenyl or C 1-10 alkylene moieties, or a combination thereof, or an amino acid chain, so as to cause reaction of the activator with the amino group on the carrier; and treating the resultant activated carrier with the protein, or a peptide having a sequence corresponding to at least part of the sequence of the protein, the protein or peptide having a sulfhydryl group thereon, thereby reacting the maleiimide group of the activator with the sulfhydryl group on the protein or peptide and producing the antigen.
Claim 4
Dependent Claim
186 words
A process for preparing an antigen for provoking the formation, in the body of an animal, of antibodies to a protein which is not endogenous or substantially immunogenic to the animal, the process being characterized by: procuring a carrier which is biologically foreign to the animal, has a size sufficient to elicit antibody response following the administration thereof into the body of the animal and does not have a sulfhydryl group but has an amino group; treating the carrier under neutral or acid conditions with an activator of the structure wherein X represents a non-reacting group comprising substituted or unsubstituted phenyl or C 1-10 alkylene moieties, or a combination thereof, or an amino acid chain, so as to cause reaction of the activator with the amino group on the carrier; and treating the resultant activated carrier with the protein, or a peptide having a sequence corresponding to at least part of the sequence of the protein, the protein or peptide having a sulfhydryl group thereon, thereby reacting the maleiimide group of the activator with the sulfhydryl group on the protein or peptide and producing the antigen.
Claim 5
Dependent Claim
159 words
A process for preparing an antigen for provoking the formation, in the body of an animal, of antibodies to a protein which is not endogenous or substantially immunogenic to the animal, the process being characterized by: procuring a carrier which is biologically foreign to the animal, has a size sufficient to elicit antibody response following the administration thereof into the body of the animal and does not have a sulfhydryl group but has an amino group; treating the carrier with an activator present as an active ester of chloro-, dichloro-, bromo- or iodo-acetic acid so as to cause reaction with the amino group on the carrier; and treating the resultant activated carrier with the protein, or a peptide having a sequence corresponding to at least part of the sequence of the protein, the protein or peptide having a sulfhydryl group thereon, thereby reacting the activated carrier with the sulfhydryl group on the protein or peptide and producing the antigen.
Claim 6
Dependent Claim
159 words
A process for preparing an antigen for provoking the formation, in the body of an animal, of antibodies to a protein which is not endogenous or substantially immunogenic to the animal, the process being characterized by: procuring a carrier which is biologically foreign to the animal, has a size sufficient to elicit antibody response following the administration thereof into the body of the animal and does not have a sulfhydryl group but has an amino group; treating the carrier with an activator present as an active ester of chloro-, dichloro-, bromo- or iodo-acetic acid so as to cause reaction with the amino group on the carrier; and treating the resultant activated carrier with the protein, or a peptide having a sequence corresponding to at least part of the sequence of the protein, the protein or peptide having a sulfhydryl group thereon, thereby reacting the activated carrier with the sulfhydryl group on the protein or peptide and producing the antigen.
Claim 7
Dependent Claim
168 words
A process for preparing an antigen for provoking the formation, in the body of an animal, of antibodies to a protein which is not endogenous or substantially immunogenic to the animal, the process being characterized by: procuring the protein, or a peptide having a sequence corresponding to at least part of the sequence of the protein, the protein or peptide not having a sulfhydryl group but having an amino group thereon; treating the protein or peptide with an activator present as an active ester of chloro-, dichloro-, bromo- or iodo-acetic acid, thereby reacting the active ester with the amino group on the protein or peptide to produce a halomethyl alkylating group containing moiety; and treating the halomethyl alkylating group containing moiety with a carrier which is biologically foreign to the animal, has a size sufficient to elicit antibody response following the administration thereof into the body of the animal and contains a sulfhydryl group, thereby reacting the sulfhydryl group with the halomethyl alkylating group to form the antigen.
Claim 8
Dependent Claim
168 words
A process for preparing an antigen for provoking the formation, in the body of an animal, of antibodies to a protein which is not endogenous or substantially immunogenic to the animal, the process being characterized by: procuring the protein, or a peptide having a sequence corresponding to at least part of the sequence of the protein, the protein or peptide not having a sulfhydryl group but having an amino group thereon; treating the protein or peptide with an activator present as an active ester of chloro-, dichloro-, bromo- or iodo-acetic acid, thereby reacting the active ester with the amino group on the protein or peptide to produce a halomethyl alkylating group containing moiety; and treating the halomethyl alkylating group containing moiety with a carrier which is biologically foreign to the animal, has a size sufficient to elicit antibody response following the administration thereof into the body of the animal and contains a sulfhydryl group, thereby reacting the sulfhydryl group with the halomethyl alkylating group to form the antigen.
Claim 9
Dependent Claim
49 words
A vaccine for provoking the formation, in the body of an animal, of antibodies to a protein, the vaccine being characterized in that it comprises an antigen prepared according to any one of the preceding claims and a vehicle comprising a mixture of mannide monooleate with Squalane and/or Squalene.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.