EP0209068A1
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EP
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EEL growth hormone
Assignee: KH Neochem Co Ltd
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Filing: 1986-07-09
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Granted: —
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Expires: 2006-07-09
Abstract
Abstract According to the present invention, a fish growth hormone was isolated from an organ culture broth of the pituitary gland of eels, and further a recombinant DNA incorporated with a DNA coding for the eel growth hormone polypeptide and a microorganism containing the recombinant DNA were obtained. They can be used for mass production of an eel growth hormone
Inventors
- 👤 Kazuo Yamaguchi
- 👤 Akiko Saito
- 👤 Susumu Sekine
- 👤 Moriyuki Sato
- 👤 Seiga Itoh
- 👤 Kunikatsu Shirahata
- 👤 Tetsuya Hirano
- 👤 Hiroshi Kawauchi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 15:19
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/73
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
18 words
A fish growth hormone which is derived from the pituitary gland of a fish belonging to Actinopteryqii Anquilliformes.
Claim 2
Dependent Claim
18 words
A fish growth hormone which is derived from the pituitary gland of a fish belonging to Actinopteryqii Anquilliformes.
Claim 3
Dependent Claim
33 words
The fish growth hormone according to claim 1, which comprises a purified extract from an organ culture broth of the pituitary gland of a fish belonging to Actino p ter yq ii Anquilliformes.
Claim 4
Dependent Claim
91 words
The fish growth hormone according to claim 2, which is a polypeptide having the following physicochemical properties: (i) Amino acid composition: as indicated in Column A, Table l; (ii) The sequence of the amino acids is as follows: (iii) Molecular weight : about 23,000; (iv) Isoelectric point: 6.3; (v) Soluble in an alkaline aqueous solution and hardly soluble or insoluble in neutral and acidic aqueous solutions; (vi) Classification as basic or acidic properties: acidic polypeptide; (vii) Color and form of substance: white powder; and (viii) Polyacrylamide gel electrophoresis: a sigle band.
Claim 5
Dependent Claim
97 words
The fish growth hormone according to claim 2, which is a polypeptide having the following physicochemical properties: (i) Amino acid composition: as indicated in Column B, Table 1; (ii) The sequences of amino acids at the N-terminal and C-terminal are as follows: N-terminal: C-terminal: (iii) Molecular weight : about 23,000; (iv) Isoelectric point: 6.7; (v) Soluble in an alkaline aqueous solution and hardly soluble or insoluble in neutral and acidic aqueous solutions; (vi) Classification as basic or acidic properties: acidic polypeptide; (vii) Color and form of substance: white powder; and (viii) Polyacrylamide gel electrophoresis: a single band.
Claim 6
Dependent Claim
15 words
The fish growth hormone according to claim 1, which is produced by recombinant DNA techniques.
Claim 7
Dependent Claim
25 words
The fish growth hormone according to claim 5, which is a polypeptide encoded by the base numbers 58-627 of the nucleotide sequence as illustrated below:
Claim 8
Dependent Claim
27 words
The fish growth hormone according to claim 5, which is a polypeptide encoded by the base numbers 67-627 of the nucleotide sequence as illustrated in claim 6.
Claim 9
Dependent Claim
16 words
The fish growth hormone according to claim 1, wherein the fish is Anquilla ia p onica.
Claim 10
Dependent Claim
46 words
A process for producing a fish growth hormone, which comprises subjecting an organ culture broth of the pituitary gland of a fish belonging to Actinopterygii Anguilliformes to purification by the conventional method to be used in the polypeptide purification, and recovering the fish growth hormone therefrom.
Claim 11
Dependent Claim
46 words
A process for producing a fish growth hormone, which comprises subjecting an organ culture broth of the pituitary gland of a fish belonging to Actinopterygii Anguilliformes to purification by the conventional method to be used in the polypeptide purification, and recovering the fish growth hormone therefrom.
Claim 12
Dependent Claim
56 words
A process for producing a fish growth hormone polypeptide, which comprises culturing in a nutrient medium a microorganism containing a recombinant DNA wherein a DNA coding for a growth hormone polypeptide of a fish belonging to Actinopterygii An q uilliformes is incorporated, accumulating the fish growth hormone polypeptide in the medium and recovering the polypeptide therefrom.
Claim 13
Dependent Claim
56 words
A process for producing a fish growth hormone polypeptide, which comprises culturing in a nutrient medium a microorganism containing a recombinant DNA wherein a DNA coding for a growth hormone polypeptide of a fish belonging to Actinopterygii An q uilliformes is incorporated, accumulating the fish growth hormone polypeptide in the medium and recovering the polypeptide therefrom.
Claim 14
Dependent Claim
17 words
A DNA coding for a growth hormone polypeptide of a fish belonging to Actinopterygii An q uilliformes.
Claim 15
Dependent Claim
17 words
A DNA coding for a growth hormone polypeptide of a fish belonging to Actinopterygii An q uilliformes.
Claim 16
Dependent Claim
23 words
The DNA according to claim 11, wherein the fish growth hormone polypeptide has the peptide sequence as defined in claim 6 or 7.
Claim 17
Dependent Claim
23 words
A recombinant DNA wherein a DNA coding for a growth hormone polypeptide of a fish belonging to Actinopterygii An q uilliformes is incorporated.
Claim 18
Dependent Claim
23 words
A recombinant DNA wherein a DNA coding for a growth hormone polypeptide of a fish belonging to Actinopterygii An q uilliformes is incorporated.
Claim 19
Dependent Claim
24 words
The recombinant DNA according to claim 13, wherein the fish growth hormone polypeptide has the peptide sequence as defined in claim 6 or 7.
Claim 20
Dependent Claim
14 words
The recombinant DNA according to claim 13, which is named plasmid pUPAl or pUPJ24.
Claim 21
Dependent Claim
26 words
A microorganism containing a recombinant DNA wherein a DNA coding for a growth hormone polypeptide of a fish belonging to Actinopterygii An q uilliformes is incorporated.
Claim 22
Dependent Claim
26 words
A microorganism containing a recombinant DNA wherein a DNA coding for a growth hormone polypeptide of a fish belonging to Actinopterygii An q uilliformes is incorporated.
Claim 23
Dependent Claim
23 words
The microorganism according to claim 16, wherein the fish growth hormone polypeptide has the peptide sequence as defined in claim 6 or 7.
Claim 24
Dependent Claim
13 words
The microorganism according to claim 16, wherein the microorganism belongs to Escherichia coli.
Claim 25
Dependent Claim
57 words
A process for stimulating growth of a fish which comprises administering to the fish a fish growth hormone which is extracted from an organ culture broth of the pituitary gland of a fish belonging to Antino p ter yq ii An q uilliformes and purified, or a fish growth hormone which is produced by recombinant DNA techniques.
Claim 26
Dependent Claim
57 words
A process for stimulating growth of a fish which comprises administering to the fish a fish growth hormone which is extracted from an organ culture broth of the pituitary gland of a fish belonging to Antino p ter yq ii An q uilliformes and purified, or a fish growth hormone which is produced by recombinant DNA techniques.
Claim 27
Dependent Claim
21 words
The process according to claim 19, wherein the fish to which the fish growth hormone is administered belongs to Teleost (Osteichthyes).
Claim 28
Dependent Claim
19 words
The process according to claim 20, wherein the fish belongs to Clu p eiformes, Anquilliformes, Perciformes, Pleuronectiformes or Tetraodontiformes.
References Cited (Backward Citations)
EP0157423A2
Cited
EP0166444A2
Cited
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