EP0207751B1
Expired / Lapsed
EP
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Fibronectins
Assignee: Albumedix Ltd
•
Filing: 1986-06-27
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Granted: 1990-11-14
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Expires: 2006-06-27
Abstract
Abstract The polypeptide sequence of the collagen-binding and fibrin-binding parts of fibronectin and the corresponding cDNA sequences have been determined. The collagen-binding polypeptide is useful in methods of purification and the fibrin-binding polypeptide is useful for targetting therapeutic substances on natural fibrin.
Inventors
- 👤 Francisco E. Baralle
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 12:53
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; C07K2319/00; C07K2319/61; C07K2319/70
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
90 words
A process for preparing a polypeptide sequence having specific affinity for collagen or fibrin, wherein the collagen-binding sequence is the amino-acid sequence 379 to 445 shown in Figure 2 or a continuous collagen-binding part thereof and the fibrin-binding portion is the amino-acid sequence 21 to 241 shown in Figure 2 or a continuous fibrin-binding part thereof and in either case the said sequence is not colinear with and adjacent the amino acid sequences which are adjacent to and colinear with it in human fibronectin, by expression of a nucleotide sequence.
Claim 2
Dependent Claim
90 words
A process for preparing a polypeptide sequence having specific affinity for collagen or fibrin, wherein the collagen-binding sequence is the amino-acid sequence 379 to 445 shown in Figure 2 or a continuous collagen-binding part thereof and the fibrin-binding portion is the amino-acid sequence 21 to 241 shown in Figure 2 or a continuous fibrin-binding part thereof and in either case the said sequence is not colinear with and adjacent the amino acid sequences which are adjacent to and colinear with it in human fibronectin, by expression of a nucleotide sequence.
Claim 3
Dependent Claim
25 words
A process according to claim 1 wherein the polypeptide as disclosed in claim 1 is bound to a polypeptide sequence not present in human fibronectin.
Claim 4
Dependent Claim
42 words
A method of purifying a substance which comprises contacting a conjugate of that substance and a collagen-binding sequence prepared according to claim 1 with immobilized collagen so that the said conjugate binds to the said collagen, and then eluting the said conjugate.
Claim 5
Dependent Claim
28 words
A method according to claim 3 in which the said eluted conjugate is then split to remove the said collagen-binding sequence, and the said substance is then isolated.
Claim 6
Dependent Claim
31 words
A process for preparing a polypeptide comprising a fibrin-binding amino-acid sequence according to claim 1 bound to a therapeutic agent, by binding the said amino acid sequence to the therapeutic agent.
Claim 7
Dependent Claim
20 words
A process for preparing a DNA sequence coding for a polypeptide sequence according to claim 1, by mRNA-directed DNA synthesis.
Claim 8
Dependent Claim
22 words
A process for preparing a DNA sequence according to claim 6 having the structure shown at 1147 to 1351 in Figure 3b.
Claim 9
Dependent Claim
22 words
A process for preparing a DNA sequence according to claim 6 having the structure shown at 73 to 738 in Figure 3a.
Claim 10
Dependent Claim
26 words
A process for preparing a plasmid or other vector containing a DNA sequence as defined in any of claims 6 to 8, by recombinant DNA techniques.
Claim 11
Dependent Claim
17 words
A method of transforming a microorganism by inclusion of a vector prepared as claimed in claim 9.
Claim 12
Dependent Claim
17 words
A method of transforming Escherichia coli by inclusion of a vector prepared as claimed in claim 9.
References Cited (Backward Citations)
No citation records indexed.
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