EP0206014A1
Expired / Lapsed
EP
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Precipitation reagent and fluorescence polarization immunoassay using same
Assignee: Abbott Laboratories
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Filing: 1986-06-03
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Granted: —
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Expires: 2006-06-03
Abstract
Abstract A reagent useful for precipitation of protein from a biological sample is disclosed. The reagent, which is especially suitable for simultaneous precipitation of interfering protein and extraction of the analyte prior to performance of fluorescence polarization im- . munoassays for digoxin in human serum, comprises, in weight/volume percent, from about 3% to about 4% 5-sulfosalicylic acid in an aqueous solution including from about 40% to about 60% of a straight or branch chained organic alcohol having from 1 to 4 carbon atoms.
Inventors
- 👤 Philip Pei Wang
- 👤 Gloria Jane Hockerman
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-10 00:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9453; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/817; Y10S436/825; Y10T; Y10T436/00; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
51 words
A reagent useful for precipitation of protein from a biological fluid, said reagent comprising, in weighf/volume percent, from about 3% to about 4% 5-sulfosalicylic acid in an aqueous solution including from about 40% to about 60% of a straight or branch chained organic alcohol having from 1 to 4 carbon atoms.
Claim 2
Dependent Claim
51 words
A reagent useful for precipitation of protein from a biological fluid, said reagent comprising, in weighf/volume percent, from about 3% to about 4% 5-sulfosalicylic acid in an aqueous solution including from about 40% to about 60% of a straight or branch chained organic alcohol having from 1 to 4 carbon atoms.
Claim 3
Dependent Claim
22 words
The reagent of claim 1, wherein the organic alcohol is present in an amount of substantially 50% (volume/volume) of the aqueous solution.
Claim 4
Dependent Claim
11 words
The reagent of claim 1, wherein the organic alcohol is methanol.
Claim 5
Dependent Claim
23 words
The reagent of claim 1, wherein the organic alcohol is selected from the group consisting of methanol, ethanol, propanol, butanol and mixtures thereof.
Claim 6
Dependent Claim
12 words
The reagent of claim 1, wherein the biological fluid is human serum.
Claim 7
Independent Claim
73 words
A method of fluorescence polarization immunoassay for digoxin in a test sample of biological fluid, which assay includes utilizing a precipitation reagent to remove interfering proteins from the sample, characterized in that the precipitation reagent comprises, in weight/volume percent, from about 3% to about 4% 5-sulfosalicylic acid in an aqueous solution including from about 40% to about 60% of a straight or branch chained organic alcohol having from 1 to 4 carbon atoms.
Claim 8
Independent Claim
73 words
A method of fluorescence polarization immunoassay for digoxin in a test sample of biological fluid, which assay includes utilizing a precipitation reagent to remove interfering proteins from the sample, characterized in that the precipitation reagent comprises, in weight/volume percent, from about 3% to about 4% 5-sulfosalicylic acid in an aqueous solution including from about 40% to about 60% of a straight or branch chained organic alcohol having from 1 to 4 carbon atoms.
Claim 9
Dependent Claim
22 words
The method of claim 6, wherein the organic alcohol is present in an amount of substantially 50% (volume/volume) of the aqueous solution.
Claim 10
Dependent Claim
11 words
The method of claim 6, wherein the organic alcohol is methanol.
Claim 11
Dependent Claim
23 words
The method of claim 6, wherein the organic alcohol is selected from the group consisting of methanol, ethanol, propanol, butanol and mixtures thereof.
Claim 12
Dependent Claim
12 words
The method of Claim 6, wherein the biological fluid is human serum.
References Cited (Backward Citations)
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