EP0204510A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Amplification of hybridization signals by employing complementary DNA strands
Assignee: BP Corp North America Inc
•
Filing: 1986-05-29
•
Granted: —
•
Expires: 2006-05-29
Abstract
Abstract A method for assaying sample for a target ligand includes the step of contacting sample with reagent from a kit including reagent probe, amplifier strand and label strand. The reagent probe is capable of binding to the target ligand and includes a polynucleotide tail portion. The amplifier strand and label strand bind to the tail portion to form a polynucleotide network. The label strand includes a label moiety capable of detection.
Inventors
- 👤 Mark Leo Collins
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 06:17
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/682
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
101 words
A method for assaying sample for a target ligand of a biological pair comprising the steps of: contacting sample with reagent under binding conditions wherein said reagent includes reagent probe, amplifier.strand, and label strand, said reagent probe capable of binding to said target ligand and having a polynucleotide tail portion, said amplifier strand including a polynucleotide strand capable of binding to said tail portion and to a plurality of said label strands, and said label strand including a polynucleotide strand having a label moiety capable of detection, said reagent for forming a network of polynucleotide strands bound to said target ligand;
Claim 2
Independent Claim
101 words
A method for assaying sample for a target ligand of a biological pair comprising the steps of: contacting sample with reagent under binding conditions wherein said reagent includes reagent probe, amplifier.strand, and label strand, said reagent probe capable of binding to said target ligand and having a polynucleotide tail portion, said amplifier strand including a polynucleotide strand capable of binding to said tail portion and to a plurality of said label strands, and said label strand including a polynucleotide strand having a label moiety capable of detection, said reagent for forming a network of polynucleotide strands bound to said target ligand;
Claim 3
Dependent Claim
6 words
removing unbound reagent from sample; and
Claim 4
Dependent Claim
6 words
removing unbound reagent from sample; and
Claim 5
Dependent Claim
7 words
monitoring said sample for said label moiety.
Claim 6
Dependent Claim
7 words
monitoring said sample for said label moiety.
Claim 7
Dependent Claim
95 words
The method of Claim 1 wherein said reagent includes a second amplifier strand and a second label strand, said second amplifier strand capable of binding to said first amplifier strand and said second label strand capable of binding to said second amplifier strand and including a label moiety, said method further comprising the additional step prior to monitoring and after contacting said sample with reagent the sample probe amplifier strand and label strand, comprising contacting said sample said second amplifier strand and said label strand under binding conditions to form a network of polynucleotide strands.
Claim 8
Dependent Claim
21 words
The method of Claim 1 or 2 wherein said amplifier strand is capable of binding to a plurality of label strands.
Claim 9
Independent Claim
23 words
The method of any of Claims I to 3 wherein at least one oi said amplifier strand and probe strand includes label moieties.
Claim 10
Independent Claim
23 words
The method of any of Claims 1 to 4 wherein said regent probe includes a polynucleotide probe portion complementary to said target ligand.
Claim 11
Independent Claim
26 words
The method of any of Claims I to 5 wherein said tail portion of said reagent probe, said amplifier strand, and said label strand include homopolymers.
Claim 12
Independent Claim
28 words
The method of any of Claims 1 to 6 wherein said reagent sample is first contacted with reagent probe, said target ligand is allowed to bind to said
Claim 13
Dependent Claim
40 words
reagent probe to form a ligand-receptor complex, followed by contacting said ligand-reagent complex with a reagent amplifier strand and reagent label strand wherein said amplifier strand and label strand are bound to each other to form an amplifier label complex.
Claim 14
Dependent Claim
40 words
reagent probe to form a ligand-receptor complex, followed by contacting said ligand-reagent complex with a reagent amplifier strand and reagent label strand wherein said amplifier strand and label strand are bound to each other to form an amplifier label complex.
Claim 15
Independent Claim
15 words
The method of any of Claims 1 to 7 wherein said label moiety is nonradioactive.
Claim 16
Dependent Claim
71 words
A reagent composition for enhancing the signal-generating capabilities of a reagent probe having a polynucleotide tail portion comprising: a reagent amplifierlabel complex including polynucleotide amplifier strand and a plurality of label strands bound thereto, said amplifier strand capable of binding to a reagent probe, said label having a label moiety capable of detection, said amplifier-label complex for binding to said reagent probe forming a network of polynucleotide strands capable of detection.
Claim 17
Dependent Claim
71 words
A reagent composition for enhancing the signal-generating capabilities of a reagent probe having a polynucleotide tail portion comprising: a reagent amplifierlabel complex including polynucleotide amplifier strand and a plurality of label strands bound thereto, said amplifier strand capable of binding to a reagent probe, said label having a label moiety capable of detection, said amplifier-label complex for binding to said reagent probe forming a network of polynucleotide strands capable of detection.
Claim 18
Dependent Claim
21 words
The reagent composition of Claim 9 wherein at least one of said amplifier strand and said probe strand includes label moieties.
Claim 19
Dependent Claim
18 words
The reagent composition of Claim 9 or 10 wherein said amplifier strand and label strand are complementary homopolymers.
Claim 20
Dependent Claim
92 words
A kit for assaying sample for target ligand of a biological pair comprising: reagent including reagent probe, amplifier strand and label strand, said reagent probe capable of binding to said target ligand and having a polynucleotide tail portion, said amplifier strand including a polynucleotide strand capable of binding to said polynucleotide tail of said reagent probe, and, said label strand capable of binding to said amplifier strnd and having a label moiety capable of detecting said reagent for forming a network of polynucleotide strands capable of detection bound to said target ligand.
Claim 21
Dependent Claim
92 words
A kit for assaying sample for target ligand of a biological pair comprising: reagent including reagent probe, amplifier strand and label strand, said reagent probe capable of binding to said target ligand and having a polynucleotide tail portion, said amplifier strand including a polynucleotide strand capable of binding to said polynucleotide tail of said reagent probe, and, said label strand capable of binding to said amplifier strnd and having a label moiety capable of detecting said reagent for forming a network of polynucleotide strands capable of detection bound to said target ligand.
Claim 22
Dependent Claim
41 words
The kit of Claim 12 further comprising a second amplifier strand and a second label strand, said second amplifier strand capable of binding to said first amplifier strand and said second label strand capable of binding to said second amplifier strand.
Claim 23
Dependent Claim
21 words
The kit of Claim 12 or 13 wherein said reagent probe includes a polynucleotide probe portion complementary to said target ligand.
Claim 24
Dependent Claim
26 words
The kit of any of Claims 12 to 14 wherein said tail portion of said reagent probe, said amplifier strand and said label strand include homopolymers.
Claim 25
Dependent Claim
26 words
The kit of any of Claims 12 to 15 wherein said amplifier strand and said label strand are bound to each other in an amplifier-label complex.
Claim 26
Dependent Claim
15 words
The kit of any of Claims 12 to 16 wherein said label moiety is nonradioactive.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.