EP0204442A2
Expired / Lapsed
EP
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Copolymer production
Assignee: Monsanto Co
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Filing: 1986-05-09
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Granted: —
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Expires: 2006-05-09
Abstract
Abstract Copolymers of poly(β-hydroxybutyric acid) and poly(β-hydroxyvaleric acid) are produced by culturing alcohol-utilising strains of Alcaligenes eutrophus on a carbon source including primary alcohols having an odd number of carbon atoms such as propan-1-ol.
Inventors
- 👤 Peter James Senior
- 👤 Stephn Hugh Collins
- 👤 Kenneth Raymond Richardson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 19:06
CPC Classifications
C; C08; C08G; C08G63/00; C08G63/02; C08G63/06; C12; C12P; C12P7/00; C12P7/62; C12P7/625
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
74 words
.A process for producing a PHB/PHV copolymer comprising cultivating an alcohol-utilising Alcaligenes eutrophus strain, that is capable of accumulating poly β-hydroxybutyric acid, on a substrate under such conditions that the micro-organism accumulates at least 10% by weight of copolymer, wherein for at least part of the time when the micro-organism is cultivated under the copolymer-accumulating conditions, the substrate comprises at least one primary alcohol, other than methanol, having an odd number of carbon atoms.
Claim 2
Dependent Claim
74 words
.A process for producing a PHB/PHV copolymer comprising cultivating an alcohol-utilising Alcaligenes eutrophus strain, that is capable of accumulating poly β-hydroxybutyric acid, on a substrate under such conditions that the micro-organism accumulates at least 10% by weight of copolymer, wherein for at least part of the time when the micro-organism is cultivated under the copolymer-accumulating conditions, the substrate comprises at least one primary alcohol, other than methanol, having an odd number of carbon atoms.
Claim 3
Dependent Claim
12 words
A process according to claim 1 wherein the primary alcohol is propan-1-ol.
Claim 4
Dependent Claim
37 words
A process according to either claim 1 or claim 2 wherein the primary alcohol provides a carbon content of at least 10% by weight of the total carbon content of the substrate present during the copolymer accumulation.
Claim 5
Dependent Claim
33 words
A process according to claim 3 wherein the primary alcohol provides a carbon content of at least 25% by weight of the total carbon content of the substrate present during the copolymer accumulation.
Claim 6
Dependent Claim
27 words
A process according to any one of claims 1 to 4 wherein the alcohol-utilising Alcaligenes eutrophus strain is Alcaligenes eutrophus CBS 388.76 or Alcaligenes eutrophus NCIB 12080.
Claim 7
Dependent Claim
66 words
A process according to any one of claims 1 to 5, which comprises in a first stage culturing the alcohol-utilising Alcaligenes eutrophus strain on an assimilable carbon source in an aqueous medium comprising sufficient of an assimilable nitrogen source to support a concentration of at least 5 gl -1 of non-copolymer cell material and in a subsequent second stage containing culturing under conditions of nitrogen starvation.
Claim 8
Dependent Claim
8 words
Alcaligenes eutrophus NCIB 12080 or a mutant thereof.
Claim 9
Dependent Claim
8 words
Alcaligenes eutrophus NCIB 12080 or a mutant thereof.
Claim 10
Dependent Claim
41 words
A process for preparing Alcaligenes eutrophus NCIB . 12080 or a mutant thereof which comprises cultivating a glucose-utilising strain of Alcaligenes eutrophus under oxygen limitation, and subsequently under carbon limitation on a substrate comprising glucose and a progressively increasing proportion of'ethanol.
Claim 11
Dependent Claim
41 words
A process for preparing Alcaligenes eutrophus NCIB . 12080 or a mutant thereof which comprises cultivating a glucose-utilising strain of Alcaligenes eutrophus under oxygen limitation, and subsequently under carbon limitation on a substrate comprising glucose and a progressively increasing proportion of'ethanol.
Claim 12
Dependent Claim
74 words
A process for producing a PHB/PHV copolymer comprising cultivating an alcohol-utilising Alcaligenes eutrophus strain, that is capable of accumulating poly p-hydroxybutyric acid, on a substrate under such conditions that the micro-organism accumulates at least 10% by weight of copolymer, wherein for at least part of the time when the micro-organism is cultivated under the copolymer-accumulating conditions, the substrate comprises at least one primary alcohol, other than methanol, having an odd number of carbon atoms.
Claim 13
Dependent Claim
74 words
A process for producing a PHB/PHV copolymer comprising cultivating an alcohol-utilising Alcaligenes eutrophus strain, that is capable of accumulating poly p-hydroxybutyric acid, on a substrate under such conditions that the micro-organism accumulates at least 10% by weight of copolymer, wherein for at least part of the time when the micro-organism is cultivated under the copolymer-accumulating conditions, the substrate comprises at least one primary alcohol, other than methanol, having an odd number of carbon atoms.
Claim 14
Dependent Claim
12 words
A process according to claim 1 wherein the primary alcohol is propan-1-ol.
Claim 15
Dependent Claim
37 words
A process according to either claim 1 or claim 2 wherein the primary alcohol provides a carbon content of at least 10% by weight of the total carbon content of the substrate present during the copolymer accumulation.
Claim 16
Dependent Claim
33 words
A process according to claim 3 wherein the primary alcohol provides a carbon content of at least 25% by weight of the total carbon content of the substrate present during the copolymer accumulation.
Claim 17
Dependent Claim
27 words
A process according to any one of claims 1 to 4 wherein the alcohol-utilising Alcaligenes eutrophus strain is Alcaligenes eutrophus CBS 388.76 or Alcaligenes eutrophus NCIB 12080.
Claim 18
Dependent Claim
66 words
A process according to any one of claims 1 to 5, which comprises in a first stage culturing the alcohol-utilising Alcaligenes eutrophus strain on an assimilable carbon source in an aqueous medium comprising sufficient of an assimilable nitrogen source to support a concentration of at least 5 gl -1 of non-copolymer cell material and in a subsequent second stage containing culturing under conditions of nitrogen starvation.
Claim 19
Dependent Claim
41 words
A process for preparing Alcaligenes eutrophus NCIB 12080 or a mutant thereof which comprises cultivating a glucose-utilising strain of Alcaligenes eutrophus under oxygen limitation, and subsequently under carbon limitation on a substrate comprising glucose and a progressively increasing proportion of ethanol.
Claim 20
Dependent Claim
41 words
A process for preparing Alcaligenes eutrophus NCIB 12080 or a mutant thereof which comprises cultivating a glucose-utilising strain of Alcaligenes eutrophus under oxygen limitation, and subsequently under carbon limitation on a substrate comprising glucose and a progressively increasing proportion of ethanol.
References Cited (Backward Citations)
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