EP0202894A2
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A method for detecting amino acid derivatives
Assignee: Seiko Instruments Inc
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Filing: 1986-05-16
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Granted: —
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Expires: 2006-05-16
Abstract
Abstract A method for detecting amino acid derivatives in which 5-thiazolinone derivatives of amino acids are reacted with radioactive iodine isotope-labelled amino compounds or with fluorescent amino compounds to form phenylthiocarbamylamino acid derivatives, the phenylithiocarbamylamino acid derivatives being detected with a high level of sensitivity by a gamma ray detector or a fluorescence spectrophotometer.
Inventors
- 👤 Akira Tsugita
- 👤 Isamu Arai
- 👤 Tatsuaki Seiko Instr.&Electronics Ltd. Ataka
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 11:51
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/12; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/534; G01N33/68; G01N33/6803; G01N33/6806; G01N33/6809; G01N33/6818; G01N33/6824; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/141111
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
81 words
A method for detecting amino acid derivatives, which method is characterised by reacting 5-thiazolinone amino acid derivatives of the general formula (a) below, with an amino compound which is of the general formula (b) below and which is labelled with a radioactive iodine isotope or which is a fluorescent amino compound, to form phenylthiocarbamylamino acid derivatives of the general formula (c) below, and detecting the phenylthiocarbamylamino acid derivatives wherein X is a residue of a radioactive iodine isotope-labelled or fluorescent compound.
Claim 2
Independent Claim
81 words
A method for detecting amino acid derivatives, which method is characterised by reacting 5-thiazolinone amino acid derivatives of the general formula (a) below, with an amino compound which is of the general formula (b) below and which is labelled with a radioactive iodine isotope or which is a fluorescent amino compound, to form phenylthiocarbamylamino acid derivatives of the general formula (c) below, and detecting the phenylthiocarbamylamino acid derivatives wherein X is a residue of a radioactive iodine isotope-labelled or fluorescent compound.
Claim 3
Dependent Claim
70 words
A method according to claim 1, characterised in that the 5-thiazolinone amino acid derivatives are obtained by reacting phenyl isothiocyanate of the general formula (d) below with a protein or a peptide of the general formula (e) below to form phenylthiocarbamylprotein of the general formula - (f) below, and reacting the phenylthiocarbamylprotein with an acid in an anhydrous condition according to the formula [III] below to effect cyclization and scission:
Claim 4
Dependent Claim
26 words
A method according to claim 1 or claim 2, characterised in that the amino compound of the general formula (b) is a radioactive iodine isotope-labelled iodohistamine.
Claim 5
Independent Claim
33 words
A method according to any one of the preceding claims, characterised in that the amino compound of the general formula (b) is a fluorescent amino compound in the form of aminopyrene or aminofluorene.
Claim 6
Independent Claim
29 words
A method according to any one of the preceding claims, characterised in that the phenylthiocarbamylamino acid derivatives are detected by using a gamma ray detector or a fluorescence spectrophotometer.
Claim 7
Independent Claim
47 words
A method for detecting amino acids or amino acid derivatives, which method is characterised by reacting thiazoline derivatives with an amino compound to form phenylthiocarbamylamino acid derivatives, and detecting the phenylthiocarbamylamino acid derivatives, the amino compound being a radioactive isotope-labelled amino compound or a fluorescent amino compound.
Claim 8
Independent Claim
47 words
A method for detecting amino acids or amino acid derivatives, which method is characterised by reacting thiazoline derivatives with an amino compound to form phenylthiocarbamylamino acid derivatives, and detecting the phenylthiocarbamylamino acid derivatives, the amino compound being a radioactive isotope-labelled amino compound or a fluorescent amino compound.
Claim 9
Independent Claim
52 words
A method for detecting amino acid derivatives, comprising reacting 5-thiazolinone derivatives (a) of amino acids with a radioactive iodine isotope labelled or fluorescent amino compound (b) according to formula [I] to form phenylthiocarbamylamino acid derivatives (c) and detecting them wherein X is a residue of a radioactive iodine isotope-labelled or fluorescent compound.
Claim 10
Independent Claim
52 words
A method for detecting amino acid derivatives, comprising reacting 5-thiazolinone derivatives (a) of amino acids with a radioactive iodine isotope labelled or fluorescent amino compound (b) according to formula [I] to form phenylthiocarbamylamino acid derivatives (c) and detecting them wherein X is a residue of a radioactive iodine isotope-labelled or fluorescent compound.
References Cited (Backward Citations)
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