EP0202688A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method and composition for detecting analyte moieties
Assignee: Enzo Biochem Inc
•
Filing: 1986-05-23
•
Granted: —
•
Expires: 2006-05-23
Abstract
Abstract Described are a method and composition for detecting analyte moieties by means of a signalling moiety capable of aggrandizement are disclosed. The signalling moiety can be attached to or not attached to the analyte moiety/analyte-specific moiety complex. The signalling moiety can be viable or non-viable. The methods disclosed herein provide a sensitive assay for the detection of a wide range of different analyte moieties.
Inventors
- 👤 Solomon Mowshowitz
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 22:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; C; C12; C12Q; C12Q1/00; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/8215; Y10S435/822; Y10S435/88; Y10S435/881; Y10S435/882; Y10S435/883; Y10S436/00; Y10S436/802
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
216 words
A method for detecting the presence of an analyte moiety comprising the steps of: a. forming a complex selected from the group consisting of: (i) said analyte moiety complexed to an analyte-specific moiety wherein at least said analyte moiety or said analyte-specific moiety is capable of independent aggrandizement, (ii) said analyte moiety complexed to said analyte-specific moiety wherein said complex further comprises an entity selected from the group consisting of a first signalling moiety capable of independent aggrandizement, a catalyst capable of converting a precursor into a nutrient, and a carrier that contains nutrients, wherein said entity is attached to said complex; and b. detecting the presence of said analyte moiety by means selected from the group consisting of: (i) said analyte moiety wherein said analyte moiety is capable of independent aggrandizement; (ii) said analyte-specific moiety wherein said analyte-specific moiety is capable of independent aggrandzement; (iii) said first signalling moiety capable of independent aggrandizement; (iv) said catalyst by contacting said catalyst with a precursor to produce a nutrient and contacting said nutrient with a second signalling moiety that is capable of aggrandizement upon contact with said nutrient; and (v) said carrier that contains nutrients by contacting said carrier with a third signalling moiety that is capable of aggrandizement upon contact with the contents of said carrier.
Claim 2
Independent Claim
216 words
A method for detecting the presence of an analyte moiety comprising the steps of: a. forming a complex selected from the group consisting of: (i) said analyte moiety complexed to an analyte-specific moiety wherein at least said analyte moiety or said analyte-specific moiety is capable of independent aggrandizement, (ii) said analyte moiety complexed to said analyte-specific moiety wherein said complex further comprises an entity selected from the group consisting of a first signalling moiety capable of independent aggrandizement, a catalyst capable of converting a precursor into a nutrient, and a carrier that contains nutrients, wherein said entity is attached to said complex; and b. detecting the presence of said analyte moiety by means selected from the group consisting of: (i) said analyte moiety wherein said analyte moiety is capable of independent aggrandizement; (ii) said analyte-specific moiety wherein said analyte-specific moiety is capable of independent aggrandzement; (iii) said first signalling moiety capable of independent aggrandizement; (iv) said catalyst by contacting said catalyst with a precursor to produce a nutrient and contacting said nutrient with a second signalling moiety that is capable of aggrandizement upon contact with said nutrient; and (v) said carrier that contains nutrients by contacting said carrier with a third signalling moiety that is capable of aggrandizement upon contact with the contents of said carrier.
Claim 3
Dependent Claim
55 words
The method of Claim 1 wherein said detecting step is carried out by means selected from the group consisting of: (a) aggrandizing said analyte moiety wherein said analyte moiety is capable of independent aggrandizement, (b) aggrandizing said analyte-specific moiety wherein said analyte-specific moiety is capable of independent aggrandizement, and (c) aggrandizing said first signalling moiety.
Claim 4
Dependent Claim
31 words
The method of Claim 1 wherein said first signalling moiety, said second signalling moiety, and said third signalling moiety are selected from the group consisting of viable and non-viable signalling moieties.
Claim 5
Dependent Claim
21 words
The method of Claim 3 wherein said first sgnalling moiety, said second signalling moiety, and said third signalling moiety are viable.
Claim 6
Independent Claim
58 words
A method for ascertaining the presence of an analyte moiety comprising: a. forming an entity comprising: a complex comprising said analyte moiety complexed to an analyte-specific moiety; a signalling moiety attached to said complex, wherein said signalling moiety is capable of independent aggrandizement; and b. detecting the presence of said analyte moiety by means of said sgnalling moiety.
Claim 7
Independent Claim
58 words
A method for ascertaining the presence of an analyte moiety comprising: a. forming an entity comprising: a complex comprising said analyte moiety complexed to an analyte-specific moiety; a signalling moiety attached to said complex, wherein said signalling moiety is capable of independent aggrandizement; and b. detecting the presence of said analyte moiety by means of said sgnalling moiety.
Claim 8
Dependent Claim
20 words
The method according to Claim 5 wherein said signalling moiety capable of independent aggrandizement is attached to said analyte-specific moiety.
Claim 9
Dependent Claim
16 words
The method according to Claim 5 wherein said signalling moiety capable of independent aggrandizement is viable.
Claim 10
Dependent Claim
26 words
The method according to Claim 7 wherein said viable signalling moiety is selected from the group consisting of microorganisms, fungi, algae, plant cells, and animal cells.
Claim 11
Dependent Claim
28 words
The method according to Claim 8 wherein said microorganism is selected from the group consisting of Staphylococci, mycoplasma, E. coli, bacillii, streptomyces, candida, viruses, mycoplasma, rickettsiae, and phages.
Claim 12
Dependent Claim
29 words
The method according to Claim 5 wherein the detection of said analyte moiety by means of said signalling moiety is carried out by the aggrandizement of said signalling moiety.
Claim 13
Dependent Claim
26 words
The method according to Claim 5 wheren said signalling moiety capable of independent aggrandizement is attached to said complex by means of a signalling moiety-specific moiety.
Claim 14
Dependent Claim
30 words
The method according to Claim 11 wherein said signalling moiety-specific moiety is selected from the group consisting of proteins, antibodies, antigens, receptors, hormones, polypeptides, ligands, haptens, polynucleotides, and nucleio acids.
Claim 15
Dependent Claim
37 words
The method according to Claim 5 wherein said analyte moiety is selected from the group consisting of ligands, receptors, proteins, antibodies, antigens, nucleic acids, polynucleotides, microorganisms, viruses, phages, bacteria, fungi, algae, allergens, animal cells, and plant cells.
Claim 16
Dependent Claim
13 words
The method according to Claim 5 wherein said analyte moiety is a polynucleotide.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.