Patent US4457791A successfully refreshed from Google Patents.
Patent US4151077A successfully refreshed from Google Patents.
EP0201751A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
4'-aminomethyfluorescein derivatives for use in fluorescence polarization immunoassys
Assignee: Abbott Laboratories
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Filing: 1986-04-17
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Granted: —
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Expires: 2006-04-17
Abstract
Abstract The present invention is directed to an improved method for the preparation of 4'-aminomethylfluorescein derivatives for use in fluorescence polarization immunoassays. The invention is also directed to a novel class of 4'-N-alkylaminomethylfluorescein derivatives possessing utility as reagents in such assays.
Inventors
- 👤 James Robert Fino
- 👤 Mohammed Tyebji Shipchandler
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 11:01
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; C; C09; C09B; C09B11/00; C09B11/04; C09B11/06; C09B11/08; C09B11/10; G01N33/531; G01N33/532; G01N33/533
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
72 words
A compound having the formula: and the acid additional salts thereof, wherein R is C 1 to C 8 alkyl; R 2 is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody; R 3 is hydrogen, alkyl, halo, amino or carboxyl; R 4 is hydrogen, alkyl, halo, amino or carboxy; and R 5 is hydrogen, alkyl, halo, amine or carboxyl.
Claim 2
Dependent Claim
72 words
A compound having the formula: and the acid additional salts thereof, wherein R is C 1 to C 8 alkyl; R 2 is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody; R 3 is hydrogen, alkyl, halo, amino or carboxyl; R 4 is hydrogen, alkyl, halo, amino or carboxy; and R 5 is hydrogen, alkyl, halo, amine or carboxyl.
Claim 3
Dependent Claim
14 words
A compound as defined in Claim 1 wherein R 1 is a methyl group.
Claim 4
Dependent Claim
14 words
A compound as defined in Claim 1 wherein R 1 is an ethyl group.
Claim 5
Dependent Claim
14 words
A compound as defined in Claim 1 wherein R 1 is an n-butyl group.
Claim 6
Dependent Claim
75 words
A process for the preparation of a compound having the formula: and the acid addition salts thereof, wherein R is C 1 to C 8 alkyl; R 3 is hydrogen, alkyl, halo, amino or carboxy; R 4 is hydrogen, alkyl, halo, amino or carboxyl; and R 5 is hydrogen, alkyl, halo, amino or carboxy; comprising reacting a haloacetauaidomethylfluorescein the the presence of an alcohol and a strong acid and recovering the 4'-N-alkylaminomethylfluorescein compound thus produced.
Claim 7
Dependent Claim
75 words
A process for the preparation of a compound having the formula: and the acid addition salts thereof, wherein R is C 1 to C 8 alkyl; R 3 is hydrogen, alkyl, halo, amino or carboxy; R 4 is hydrogen, alkyl, halo, amino or carboxyl; and R 5 is hydrogen, alkyl, halo, amino or carboxy; comprising reacting a haloacetauaidomethylfluorescein the the presence of an alcohol and a strong acid and recovering the 4'-N-alkylaminomethylfluorescein compound thus produced.
Claim 8
Dependent Claim
12 words
A process as defined in Claim 5 wherein the alcohol is methanol.
Claim 9
Dependent Claim
12 words
A process as defined in Claim 5 wherein the alcohol is ethanol.
Claim 10
Dependent Claim
12 words
A process as defined in Claim 5 wherein the alcohol is n-butanol.
Claim 11
Dependent Claim
28 words
In a method of detecting a ligand by fluorescence polarization using a fluorescent tracer, the improvement comprising using as a tracer a compound as defined in Claim 1.
Claim 12
Dependent Claim
38 words
A process for the preparation of 4'-aminomethylfluorescein compounds, and the acid addition salts thereof, comprising reacting an acetamidomethylfluorescein or 4'-haloacetamidomethylfluorescein in the presence of a nonhydroxylic ether and a strong acid and recovering the 4'-aminomethylfluorescein compound thus produced.
Claim 13
Dependent Claim
38 words
A process for the preparation of 4'-aminomethylfluorescein compounds, and the acid addition salts thereof, comprising reacting an acetamidomethylfluorescein or 4'-haloacetamidomethylfluorescein in the presence of a nonhydroxylic ether and a strong acid and recovering the 4'-aminomethylfluorescein compound thus produced.
References Cited (Backward Citations)
No citation records indexed.
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