EP0201086A2
Expired / Lapsed
EP
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Method of producing useful high-molecular-weight substances by culturing proliferous animal cells and culture system therefor
Assignee: Teijin Ltd
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Filing: 1986-05-06
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Granted: —
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Expires: 2006-05-06
Abstract
Abstract @ A method of producing a useful high-molecular-weight substance by culturing animal cells capable of proliferation, which comprises (A) a culturing step of culturing said animal cells in suspension to form a suspension culture mixture containing the animal cells and a useful high-molecular-weight substance produced by the animal cells; (B) a separating step of separating the animal cells from the suspension culture mixture and withdrawing the culture medium; (C) a removing step of removing low-molecular-weight substances inhibiting the growth of the animal cells from the culture medium, said inhibitor substances being the metabolites of the animal cells and different from the useful high-molecular-weight substance; (D) a recycling step of recycling part or the whole of the culture medium left after removal of the growth-inhibiting low-molecular-weight substances to the culturing step (A); and (E) a recovering step of recovering the useful high-molecular-weight substance accumulated in the culture system. A culture system for culturing the animal cells is also described.
Inventors
- 👤 Michiyuki Tokashiki
- 👤 Kimihiko Hamamoto
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 00:14
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/10; C12N5/12; C12N5/16; C12N5/163; C07; C07K; C07K16/00; C12M; C12M29/00; C12M29/18; C12M47/00; C12M47/02; C12M47/04; C12M47/10; C12N2500/00; C12N2500/05; C12N2500/10; C12N2500/20; C12N2500/24; C12N2500/25; C12N2500/90
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
146 words
A method of producing a useful high-molecular-weight substance by culturing animal cells capable of proliferation, which comprises (A) a culturing step of culturing said animal cells in suspension to form a suspension culture mixture containing the animal cells and a useful high-molecular-weight substance produced by the animal cells; (B) a separating step of separating the animal cells from the suspension culture mixture and withdrawing the culture medium; (C) a removing step of removing low-molecular-weight substances inhibiting the growth of the animal cells from the culture medium, said inhibitor substances being the metabolites of the animal cells and different from the useful high-molecular-weight substance; (D) a recycling step of recycling part or the whole of the culture medium left after removal of the growth-inhibiting low-molecular-weight substances to the culturing step (A); and (E) a recovering step of recovering the useful high-molecular-weight substance accumulated in the culture system.
Claim 2
Independent Claim
146 words
A method of producing a useful high-molecular-weight substance by culturing animal cells capable of proliferation, which comprises (A) a culturing step of culturing said animal cells in suspension to form a suspension culture mixture containing the animal cells and a useful high-molecular-weight substance produced by the animal cells; (B) a separating step of separating the animal cells from the suspension culture mixture and withdrawing the culture medium; (C) a removing step of removing low-molecular-weight substances inhibiting the growth of the animal cells from the culture medium, said inhibitor substances being the metabolites of the animal cells and different from the useful high-molecular-weight substance; (D) a recycling step of recycling part or the whole of the culture medium left after removal of the growth-inhibiting low-molecular-weight substances to the culturing step (A); and (E) a recovering step of recovering the useful high-molecular-weight substance accumulated in the culture system.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said animal cells are hybridoma cells.
Claim 4
Dependent Claim
20 words
The method of claim 1 wherein the separating step (B) is carried out by gravity sedimentation, centrifugal sedimentaion or filtration.
Claim 5
Dependent Claim
18 words
The method of claim 1 wherein the removing step (C) is carried out by ultrafiltration, dialysis or adsorption.
Claim 6
Dependent Claim
25 words
The method of claim 1 wherein the low-molecular-weight substances inhibiting the growth of said animal cells have a molecular weight of 10,000 at the highest.
Claim 7
Dependent Claim
42 words
The method of claim 1 wherein said animal cells produce a useful high-molecular-weight substance having a molecular weight of more than 10,000 in the suspension culture mixture, and the culture fluid obtained in the removing step (C) contains said useful high-molecular-weight substance.
Claim 8
Dependent Claim
12 words
The method of claim 6 wherein the high-molecular-weight substance is a protein.
Claim 9
Dependent Claim
12 words
The method of claim 6 wherein the high-molecular-weight substance is an immunoglobulin.
Claim 10
Dependent Claim
13 words
The method of claim 6 wherein the high-molecular-weight substance is immunoglobulin G (IgG).
Claim 11
Dependent Claim
31 words
The method of claim 6 wherein the high-molecular-weight substance is a human immunoglobulin. ll. The method of claim 1 wherein the suspension culture mixture containing said animal cells is substantially serum-free.
Claim 12
Dependent Claim
26 words
The method of claim 1 wherein the suspesion culture mixture containing said animal cells contains a substance capable of promoting the growth of the animal cells.
Claim 13
Dependent Claim
40 words
The method of claim 1 wherein the suspension culture mixture containing said animal cells contains a substance capable of promoting the growth of the animal cells, and the culture fluid obtained in the removing step (C) contains the growth-promoting substance.
Claim 14
Dependent Claim
58 words
The method of claim 1 wherein the suspension culture mixture containing the proliferating animal cells contains a substance capable of promoting the growth of the animal cells, the animal cells produce a useful high-molecular-weight substance having a molecular weight of more than 10,000, and the culture fluid obtained in the removing step (C) contains said useful high-molecular-weight substance.
Claim 15
Dependent Claim
19 words
The method of claim 12 wherein the growth promoting substance is transferin, insulin, albumin, ethanolamine or a selenium compound.
Claim 16
Dependent Claim
15 words
The method of claim 13 wherein the growth promoting substance is transferin, albumin or insulin.
Claim 17
Dependent Claim
15 words
A culture system for culturing animal cells capalbe of proliferation in suspension, said system comprising
Claim 18
Dependent Claim
15 words
A culture system for culturing animal cells capalbe of proliferation in suspension, said system comprising
Claim 19
Dependent Claim
20 words
(1) a culture vessel for culturing in suspension said animal cells having the ability to produce a useful high-molecular-weight substance,
Claim 20
Dependent Claim
20 words
(1) a culture vessel for culturing in suspension said animal cells having the ability to produce a useful high-molecular-weight substance,
Claim 21
Dependent Claim
29 words
(2) a separating means for separating the animal cells from the resulting suspension culture mixture containing the animal cells and withdrawing the culture medium free of the animal cells,
Claim 22
Dependent Claim
29 words
(2) a separating means for separating the animal cells from the resulting suspension culture mixture containing the animal cells and withdrawing the culture medium free of the animal cells,
Claim 23
Dependent Claim
34 words
(3) a removing means for removing low-molecular-weight substances from the culture medium free from the animal cells, said low-molecular-weight substances being metabolites of the animal cells and inhibiting the growth of the animal cells,
Claim 24
Dependent Claim
34 words
(3) a removing means for removing low-molecular-weight substances from the culture medium free from the animal cells, said low-molecular-weight substances being metabolites of the animal cells and inhibiting the growth of the animal cells,
Claim 25
Dependent Claim
18 words
(4) a conveying means for conveying the culture medium free from the animal cells to the removing means,
Claim 26
Dependent Claim
18 words
(4) a conveying means for conveying the culture medium free from the animal cells to the removing means,
Claim 27
Dependent Claim
20 words
(5) means for recycling the culture medium left after removal of the growth-inhibiting low-molecular-weight substances to the culture vessel, and
Claim 28
Dependent Claim
20 words
(5) means for recycling the culture medium left after removal of the growth-inhibiting low-molecular-weight substances to the culture vessel, and
Claim 29
Dependent Claim
14 words
(6) means for withdrawing the culture medium for recovery of the useful high-molecular-weight substance.
Claim 30
Dependent Claim
14 words
(6) means for withdrawing the culture medium for recovery of the useful high-molecular-weight substance.
References Cited (Backward Citations)
No citation records indexed.
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