EP0194818A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Production of interleukin-2
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1986-03-07
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Granted: —
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Expires: 2006-03-07
Abstract
Abstract The present invention provides an improvement in a method for producing interleukin-2 by cultivating a transformant of Escherichia coli capable of producing interleukin-2 into a medium, which comprises inoculating said Escherichia coli into the medium of pH from 4.8 to 6.0 and growing it while keeping the pH range.By the method of the present invention, the interleukin-2 productivity is considerably improved.
Inventors
- 👤 Kazuaki Kitano
- 👤 Shigeru Fujimoto
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 22:52
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/54; C07K14/55; C12P; C12P21/00; C12P21/02
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
45 words
In a method for producing interleukin-2 which comprises cultivating a transformant of Escherichia coli capable of producing interleukin-2 in a medium, the improvement comprising inoculating said Escherichia coli into the medium of pH from 4.8 to 6.0 and growing it while keeping the pH range.
Claim 2
Dependent Claim
45 words
In a method for producing interleukin-2 which comprises cultivating a transformant of Escherichia coli capable of producing interleukin-2 in a medium, the improvement comprising inoculating said Escherichia coli into the medium of pH from 4.8 to 6.0 and growing it while keeping the pH range.
Claim 3
Dependent Claim
29 words
The method according to claim 1, wherein the transformant contains DNA coding for interleukin-2 comprising the amino acid sequence of No. 1 to No. 133 in the following figure:
Claim 4
Dependent Claim
13 words
The method according to claim 2, wherein the DNA codes for the interleukin-2.
Claim 5
Dependent Claim
16 words
The method according to claim 2, wherein the transformant contains a promoter upstream from the DNA.
Claim 6
Dependent Claim
16 words
The method according to claim 1, wherein the promoter is a tryptophane promoter or tryptophane promoters.
Claim 7
Dependent Claim
16 words
The method according to claim 1, wherein the Escherichia coli is originated from Escherichia coli K-12.
Claim 8
Dependent Claim
14 words
The method according to claim 1, wherein the pH is from 5.0 to 5.8..
Claim 9
Dependent Claim
22 words
The method according to claim 1, wherein the pH of the medium is adjusted by an inorganic base as a basic substance.
Claim 10
Dependent Claim
13 words
The method according to claim 8, wherein the inorganic base is ammonia water.
Claim 11
Dependent Claim
22 words
The method according to claim 1, wherein the medium is M-9 medium with glucose and casamic acid added thereto (modified M-9 medium).
Claim 12
Dependent Claim
15 words
The method according to claim 1, wherein the growing is conducted under aeration with agitation.
Claim 13
Dependent Claim
41 words
The method according to claim 1, wherein the growing is conducted to keep at a temperature around 37°C until the middle stage of the growth and then lowered to a temperature of from 20 to 30°C in proportion to the propagation.
References Cited (Backward Citations)
No citation records indexed.
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