EP0189688A2
Expired / Lapsed
EP
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Monoclonal antibody capable of recognizing arteriosclerotic lesions and agents for detecting and treating arteriosclerosis
Assignee: Japan Science and Technology Agency
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Filing: 1985-11-29
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Granted: —
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Expires: 2005-11-29
Abstract
Abstract Herein disclosed are a monoclonal antibody capable of preferentially recognizing arteriosclerotic lesions and prepared from a hybridoma obtained by fusing myeloma cells and cells capable of producing antibodies against arteriosclerotic lesions, such as spleen cells, peripheral lymphocyte of thymus and peripheral vascular cells, the antibody being able to use as an agent for detecting the presence of arteriosclerotic lesions in a body examined and for treating arteriosclerosis.
Inventors
- 👤 Tatsuya Takano
- 👤 Ryoichi Hashida
- 👤 Keiji Nakagami
- 👤 Junji Kimura
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 08:31
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; A; A61; A61P; A61P9/00; A61P9/10; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/808; Y10S530/809
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
30 words
A monoclonal antibody capable of specifically recognizing arteriosclerotic lesions and obtained from a hybridoma which is produced by fusing myeloma cells and cells capable of producing antibody against arteriosclerotic lesions.
Claim 2
Dependent Claim
30 words
A monoclonal antibody capable of specifically recognizing arteriosclerotic lesions and obtained from a hybridoma which is produced by fusing myeloma cells and cells capable of producing antibody against arteriosclerotic lesions.
Claim 3
Dependent Claim
37 words
A monoclonal antibody as set forth in claim 1 in which the cells producing antibody are one member selected from the group consisting of spleen cells, peripheral lymphocyte of thymus and peripheral vascular cells of arteriosclerotic mammals.
Claim 4
Dependent Claim
19 words
A monoclonal antibody as set forth in claim 1 wherein the cells producing antibody are sensitized with an antigen.
Claim 5
Dependent Claim
20 words
A monoclonal antibody as set. forth in claim 3 wherein the antigen is arterial wall from a congenital arteriosclerotic mammal.
Claim 6
Dependent Claim
15 words
A monoclonal antibody as set forth in claim 4 wherein the mammal is WHHL rabbit.
Claim 7
Dependent Claim
27 words
A monoclonal antibody as set forth in claim 1 wherein the cells producing antibody are spleen cells of BALB/c mouse sensitized with arterial wall of WHHL rabbit.
Claim 8
Dependent Claim
18 words
A monoclonal antibody as set forth in claim 1 wherein the arteriosclerotic lesion specifically recognized in atherosclerotic cells.
Claim 9
Dependent Claim
19 words
A monoclonal antibody as set forth in claim 1 in which the arteriosclerotic lesion specifically recognized is foam cells.
Claim 10
Dependent Claim
20 words
A monoclonal antibody as set forth in claim 1 wherein the arteriosclerotic lesion specifically recognized is intercellular lipids accumulating portion.
Claim 11
Dependent Claim
54 words
A monoclonal antibody as set forth in claim 1 wherein the hybridoma is produced by immunizing mouse with a homogenate, as the antigen, prepared from lesional sections of an arteriosclerotic mammal, fusing spleen cells from the sensitized mouse and myeloma cells to produce a mixture of hybridomas, culturing, assaying and, then cloning the mixture.
Claim 12
Dependent Claim
20 words
A monoclonal antibody as set forth in claim 10 wherein the hybridoma cloned is artificially cultured in a culture medium.
Claim 13
Dependent Claim
36 words
A monoclonal antibody as set forth in claim 10 wherein the hybridoma cloned is cultured in the abdominal cavity of mouse previously sensitized and then the ascites of the mouse is collected to recover immunoglobulin fraction.
Claim 14
Dependent Claim
45 words
13 An agent for detect or treating arteriosclerosis which comprises at least one antibody capable of specifically recognizing and bonding to arteriosclerotic lesions and obtained from a hybridoma which is produced by fusing myeloma cells and cells producing antibody against arteriosclerotic lesions of blood vessel.
Claim 15
Dependent Claim
45 words
13 An agent for detect or treating arteriosclerosis which comprises at least one antibody capable of specifically recognizing and bonding to arteriosclerotic lesions and obtained from a hybridoma which is produced by fusing myeloma cells and cells producing antibody against arteriosclerotic lesions of blood vessel.
Claim 16
Dependent Claim
17 words
An agent as set forth in claim 13 wherein the lesion of blood vessel is atherosclerotic cells.
Claim 17
Dependent Claim
17 words
An agent as set forth in claim 13 wherein the lesion of blood vessel is foam cells.
References Cited (Backward Citations)
No citation records indexed.
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