EP0187332B1
Expired / Lapsed
EP
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Photochemical method of labelling nucleic acids for detection in hybridization assays
Assignee: Molecular Diagnostics Inc
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Filing: 1985-12-18
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Granted: 1989-02-01
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Expires: 2005-12-18
Abstract
Abstract In a test for the presence of a particular nucleic acid in a test sample wherein the same is subjected to hybridizing conditions with a lbeled nucleic acid probe, and the product is assayed for the presence of said label, the improvement wherein said probe comprises a probe according to claim 1. A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, (c) a label and (d) a spacer chemically linking (b) and (c).
Inventors
- 👤 Nanibhushan Dattagupta
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 10:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
65 words
A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photo-chemically linked to the nucleic acid component, (c) a label and (d) a spacer chemically linking (b) and (c), characterized in that the spacer is -glycyl-glycyl-glycyl- or ―HN―CH 2 ―CH 2 ―NH― or -NH(CH 2 ) 9- CO- or ―NH―(CH 2 ) 6 ―NH― or - spermine or spermidine.
Claim 2
Dependent Claim
65 words
A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photo-chemically linked to the nucleic acid component, (c) a label and (d) a spacer chemically linking (b) and (c), characterized in that the spacer is -glycyl-glycyl-glycyl- or ―HN―CH 2 ―CH 2 ―NH― or -NH(CH 2 ) 9- CO- or ―NH―(CH 2 ) 6 ―NH― or - spermine or spermidine.
Claim 3
Dependent Claim
26 words
A probe according to claim 1, wherein the spacer is connected to at least one of (b) and (c) by a divalent radical or a coupler.
Claim 4
Dependent Claim
27 words
A probe according to claim 1, wherein the nucleic acid-binding ligand is an intercalator compound selected from acridine dyes, phenanthridines, phenazines, furoccumarins, phenothiazines and quinolines and antracyclines.
Claim 5
Dependent Claim
55 words
A probe according to claim 3, wherein the intercalator is a compound of the formula in which R i , R 2 and R 3 each independently is hydrogen or lower alkyl, and R 4 is hydrogen, lower alkyl or lower alkyl substituted by hydroxy, lower alkoxy, amino, halo and/or preferably 4'-aminomethyl-4,5'-dimethyl-angelicin of the formula
Claim 6
Dependent Claim
65 words
A probe according to claim 3, characterized in that the intercalator is a compound of the formula in which R, R 1 , R 2 and R 3 each independently is hydrogen or lower alkyl, R 4 is hydrogen, lower alkyl or lower alkyl substituted by hydroxy, lower alkoxy, amino, halo and/or and R 5 is hydrogen, hydroxy, carboxy, carbo-lower alkoxy or lower alkoxy, preferably
Claim 7
Independent Claim
103 words
A method for determination of the presence of a particular nucleic acid in a test sample wherein the sample is subjected to hybridizing conditions with a labeled nucleic acid probe, and the product is assayed for the presence of said label, wherein said probe comprises (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, (c) a label and (d) a spacer chemically linking (b) and (c), characterized in that the spacer is -glycyl-glycyl-glycyl- or ―HN―CH 2 ―CH 2 ―NH― or ―NH(CH 2 ) 5 ―CO― or -NH-(CH 2 ) 6 -NH- or spermine or spermidine.
Claim 8
Independent Claim
103 words
A method for determination of the presence of a particular nucleic acid in a test sample wherein the sample is subjected to hybridizing conditions with a labeled nucleic acid probe, and the product is assayed for the presence of said label, wherein said probe comprises (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, (c) a label and (d) a spacer chemically linking (b) and (c), characterized in that the spacer is -glycyl-glycyl-glycyl- or ―HN―CH 2 ―CH 2 ―NH― or ―NH(CH 2 ) 5 ―CO― or -NH-(CH 2 ) 6 -NH- or spermine or spermidine.
Claim 9
Dependent Claim
68 words
Use of a labeled nucleic acid probe in a hybridization technique said probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, (c) a label and (d) a spacer chemically linking (b) and (c), characterized in that the spacer is -glycyl-glycyl-glycyl- or -HN-CH 2 -CH 2 -NH- or ―NH(CH 2 ) 5 ―CO― or -NH-(CH2)6-NH- or spermine or spermidine.
Claim 10
Dependent Claim
68 words
Use of a labeled nucleic acid probe in a hybridization technique said probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, (c) a label and (d) a spacer chemically linking (b) and (c), characterized in that the spacer is -glycyl-glycyl-glycyl- or -HN-CH 2 -CH 2 -NH- or ―NH(CH 2 ) 5 ―CO― or -NH-(CH2)6-NH- or spermine or spermidine.
References Cited (Backward Citations)
No citation records indexed.
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