EP0186100A2
Expired / Lapsed
EP
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Analytical device and method for using same
Assignee: Abbott Laboratories
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Filing: 1985-12-17
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Granted: —
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Expires: 2005-12-17
Abstract
Abstract An improved, solid-phase analytical device for determining the presence or amount of a substance in a test sample, as well as an assay method using the devices is disclosed. The device comprises a porous, non-bilbulous fiber matrix impregnated with a hydrophobic polymer which forms a surface coating on at least a portion of the fibers of the matrix. The device also comprises a reagent capable of reaction with the substance in the sample; the polymer is capable of retaining the reagent within the matrix. The device of the invention is particularly advantageous for use in enzyme immunoassays.
Inventors
- 👤 Vincent Tan Yue
- 👤 Kenneth R. Houseman
- 👤 Robert George Parsons
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 13:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54391; G01N33/551; G01N33/552; G01N33/576; G01N33/5761
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
67 words
An analytical device for determining the presence or amount of a substance in a test sample, said device comprising a porous, non-bibulous fiber matrix impregnated with a hydrophobic polymer forming a surface coating on at least a portion of the fibers of the matrix, and a reagent capable of reaction with the substance in the sample, said polymer being capable of retaining said reagent within said matrix.
Claim 2
Dependent Claim
67 words
An analytical device for determining the presence or amount of a substance in a test sample, said device comprising a porous, non-bibulous fiber matrix impregnated with a hydrophobic polymer forming a surface coating on at least a portion of the fibers of the matrix, and a reagent capable of reaction with the substance in the sample, said polymer being capable of retaining said reagent within said matrix.
Claim 3
Dependent Claim
25 words
The analytical device set forth in claim 1, wherein the hydrophobic polymer is selected from the group consisting of poylstyrene, polymethylacrylate, polypropylene, polyacrylonitrile and polycarbonate.
Claim 4
Dependent Claim
16 words
The analytical device set forth in claim 1, wherein the matrix is composed of glass fibers.
Claim 5
Dependent Claim
14 words
The analytical device set forth in claim 1, wherein the reagent is an antigen.
Claim 6
Dependent Claim
14 words
The analytical device set forth in claim 1, wherein the reagant is an antibody.
Claim 7
Dependent Claim
22 words
The analytical device set forth in claim 1, wherein the device additionally comprises a removable matrix of material overlaying said fiber matrix.
Claim 8
Dependent Claim
92 words
A method for determining the presence or amount of a substance in a fluid, comprising the steps of a) applying a portion of the fluid to the fiber matrix of the device of claim 1; b) applying a reagant capable of reacting with the reagent retained by said matrix; c) applying a second reagant capable of producing a detectable response in said matrix to the presence of said reagant of step b); and d) interpreting the detectable response as an indication of the presence or amount of the substance in the fluid.
Claim 9
Dependent Claim
26 words
The method set forth in claim 7, wherein the reagant capable of producing a detectable response of a chromogen are the detectable response in color development.
Claim 10
Dependent Claim
33 words
The method set forth in claim 7, wherein the reagant retained by said matrix of the device is an antibody and the reagent of step b) is an antigen conjugated with an enzyme.
References Cited (Backward Citations)
No citation records indexed.
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