EP0183181A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Process for the analysis of haemoglobin Hba2
Assignee: Chemical Laboratories Srl
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Filing: 1985-11-21
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Granted: —
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Expires: 2005-11-21
Abstract
Abstract The invention relates to a process for the analysis of haemoglobin HbA 2 characterized in that it comprises the following steps a) mixing an anionic resin with a hemolysate of blood; b) agitation of the mixture obtained in (a), to obtain separation in two phases; c) filtration of the light phase containing haemoglobin HbA 2 ; and d) photometric analysis of the filtrate obtained in (c). The process permits the obtainment of a particularly rapid analysis of haemoglobin HbA 2 and allows the carrying out of batch analyses.
Inventors
- 👤 Giorgio Torelli
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 04:22
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
69 words
A process for the analysis of haemoglobin A (HbA 2 ) characterized in that it comprises the following steps: a) mixing an anionic resin with a hemolysate of blood; b) agitation of the mixture obtained in (a) to obtain separation of said mixture into a heavy phase and an overlying light phase; c) filtration of said light phase; and d) photometric analysis of the filtrate obtained at step (c).
Claim 2
Dependent Claim
69 words
A process for the analysis of haemoglobin A (HbA 2 ) characterized in that it comprises the following steps: a) mixing an anionic resin with a hemolysate of blood; b) agitation of the mixture obtained in (a) to obtain separation of said mixture into a heavy phase and an overlying light phase; c) filtration of said light phase; and d) photometric analysis of the filtrate obtained at step (c).
Claim 3
Dependent Claim
20 words
A process.according to claim 1 characterized in that said step (a) comprises the addition of an acqueous solution of glycine.
Claim 4
Dependent Claim
23 words
A process according to claim 2 characterized in that said acqueous solution of glycine has a concentration comprised between 0.05 and 0.3 mol/L.
Claim 5
Dependent Claim
24 words
A process according to claim 2 characterized in that said acqueous solution of glycine is stopped at a pH comprised between 7.1 and 7.6.
Claim 6
Dependent Claim
23 words
A process according to claim 2 characterized in that said anionic resin is present at a concentration comprised between 50 and 400 g/L.
Claim 7
Dependent Claim
17 words
A process according to claim 1, characterized in that said agitation is effected with a rotating agitator.
Claim 8
Dependent Claim
27 words
A process according to claim 1 characterized in that said filtration is carried out at the interior of said mixing vessel by means of a plunger filter.
Claim 9
Dependent Claim
20 words
A process according to claim 7 characterized in that said plunger has a cavity adapted for collecting the filtered product.
Claim 10
Dependent Claim
21 words
A process according to claim 1 charaoterzed in that the absorbance of the filtrate is determined Gram 480 nm to 425nm.
References Cited (Backward Citations)
No citation records indexed.
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