EP0180964A1
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EP
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Recombinant DNA, microorganism transformed therewith, and process for producing human Cu-Zn superoxide dismutase using the transformed microorganism
Assignee: Ube Corp
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Filing: 1985-11-05
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Granted: —
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Expires: 2005-11-05
Abstract
Abstract A recombinant DNA comprising a regulatory gene having a positive regulation site and joined downstream thereof, a human Cu-Zn superoxide dismutase structural gene, and a process for producing human Cu-Zn superoxide dismutase by cultivating a microorganism transformed with the recombinant DNA, and recovering the human Cu-Zn superoxide dismutase accumulated in the culture.
Inventors
- 👤 Kumahara C/O Ube Research Laboratory Hiromi
- 👤 Maruyama C/O Ube Research Laboratory Masao
- 👤 Anpeiji C/O Ube Research Laboratory Shigeharu
- 👤 Owa C/O Ube Research Laboratory Takuji
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 14:36
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0089; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C07; C07K; C07K2319/00
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
23 words
A recombinant DNA comprising a regulatory gene having a positive regulation site and joined downstream thereof, a human Cu-Zn superoxide dismutase structural gene.
Claim 2
Dependent Claim
23 words
A recombinant DNA comprising a regulatory gene having a positive regulation site and joined downstream thereof, a human Cu-Zn superoxide dismutase structural gene.
Claim 3
Dependent Claim
18 words
The recombinant DNA of claim 1 wherein the regulatory gene is the regulatory gene of colicin El gene.
Claim 4
Dependent Claim
34 words
The recombinant DNA of claim 1 or 2 wherein the codon for the N-terminus alanine of the human Cu-Zn superoxide dismutase structural gene exists immediately after the translation initiation codon of the regulatory gene.
Claim 5
Dependent Claim
29 words
The recombinant DNA of claim 1 or 2 wherein the human Cu-Zn superoxide dismutase structural gene is a complementary DNA derived from messenger RNA isolated from normal human tissues.
Claim 6
Dependent Claim
29 words
The recombinant DNA of claim 1 or 2 wherein the human Cu-Zn superoxide dismutase structural gene is a complementary DNA derived from messenger RNA isolated from the human placenta.
Claim 7
Dependent Claim
62 words
The recombinant DNA of claim 1 or 2 wherein the human Cu-Zn superoxide dismutase structural gene is a complementary DNA derived from messenger DNA separated from normal human tissues, and codons for some amino acids are replaced by codons which are frequently used in a host organism so long as the replacement does not alter the amino acid sequence of the DNA.
Claim 8
Dependent Claim
13 words
The recombinant DNA of claim 6 wherein the host organism is Escherichia coli.
Claim 9
Dependent Claim
21 words
The recombinant DNA of claim 1 or 2 wherein the human Cu-Zn superoxide dismutase structural gene has the following base sequence:
Claim 10
Dependent Claim
21 words
The recombinant DNA of claim 1 or 2 wherein the human Cu-Zn superoxide dismutase structural gene has the following base sequence:
Claim 11
Dependent Claim
27 words
A microorganism transformed with a recombinant DNA comprising a regulatory gene having a positive regulation site and joined downstream thereof, a human Cu-Zn superoxide dismutase structural gene.
Claim 12
Dependent Claim
27 words
A microorganism transformed with a recombinant DNA comprising a regulatory gene having a positive regulation site and joined downstream thereof, a human Cu-Zn superoxide dismutase structural gene.
Claim 13
Dependent Claim
12 words
ll. The microorganism of claim 10 wherein the microorganism is Escherichia coli.
Claim 14
Dependent Claim
49 words
A process for producing human Cu-Zn superoxide dismutase, which comprises cultivating a microorganism transformed with a recombinant DNA comprising a regulatory gene having a positive regulation site and joined downstream thereof, a human Cu-Zn superoxide dismutase structural gene; and recovering the human Cu-Zn superoxide dismutase accumulated in the culture.
Claim 15
Dependent Claim
49 words
A process for producing human Cu-Zn superoxide dismutase, which comprises cultivating a microorganism transformed with a recombinant DNA comprising a regulatory gene having a positive regulation site and joined downstream thereof, a human Cu-Zn superoxide dismutase structural gene; and recovering the human Cu-Zn superoxide dismutase accumulated in the culture.
Claim 16
Dependent Claim
21 words
The process of claim 12 wherein the regulatory gene in the recombinant DNA is the regulatory gene of colicin El gene.
Claim 17
Dependent Claim
13 words
The process of claim 12 or 13 wherein the microorganism is Escherichia coli.
References Cited (Backward Citations)
EP0138111B1
Cited
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