Patent US4040750A successfully refreshed from Google Patents.
EP0177352A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Improvements in or relating to enzyme-linked immunosorbent assay
Assignee: State of Victoria
•
Filing: 1985-10-03
•
Granted: —
•
Expires: 2005-10-03
Abstract
Abstract An apparatus for enzyme-linked immunosorbent assay including a vessel including a first line of wells; and a second line of wells; the first line of wells including at least one well adapted to form a control and at least one well coated with a first specific antigen, antibody or like material; the second line of wells (b) including at least one well adapted to form a control and at least one well coated with a second specific antigen, antibody or like material, each specific antigen antibody or like material being selected to recognize and bind to a sample.
Inventors
- 👤 Terence Leslie Spencer
- 👤 Ronald Mark Patterson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 11:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/02
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
108 words
1 . An apparatus for enzyme-linked immunosorbent assay including (1) a vessel including (a) a first line of wells; and (b) a second line of wells; the first line of wells (a) including (i) at least one well adapted to form a control, and (ii) at least one well coated with a first specific antigen, antibody or like material; the second line of wells (b) including (i) at least one well adapted to form a control, and (ii) at least one well coated with a second specific antigen, antibody or like material; each specific antigen, antibody or like material being selected to recognize and bind to a sample.
Claim 2
Dependent Claim
108 words
1 . An apparatus for enzyme-linked immunosorbent assay including (1) a vessel including (a) a first line of wells; and (b) a second line of wells; the first line of wells (a) including (i) at least one well adapted to form a control, and (ii) at least one well coated with a first specific antigen, antibody or like material; the second line of wells (b) including (i) at least one well adapted to form a control, and (ii) at least one well coated with a second specific antigen, antibody or like material; each specific antigen, antibody or like material being selected to recognize and bind to a sample.
Claim 3
Dependent Claim
46 words
An apparatus according to claim 1 wherein the vessel further includes (c) at least one further line of wells including (i) at least one well adapted to form a control, and (ii) at least one well coated with a further specific antigen, antibody or like material.
Claim 4
Dependent Claim
47 words
An apparatus according to claim 2 wherein each specific antigen, antibody or like material is selected from antisera including beef/buffalo- specific antiserum, a sheep-specific antiserum, a pig-specific antiserum, a horse/donkey-specific antiserum, a kangaroo-specific antiserum, a buffalo-specific antiserum, a goat-specific antiserum and a donkey-specific antiserum or mixtures thereof.
Claim 5
Dependent Claim
31 words
An apparatus according to claim 2 wherein the sample to be tested is a milk or milk product and the antisera are selected from sheep anti-cow antiserum and sheet- anti-goat antiserum.
Claim 6
Dependent Claim
52 words
5 . An apparatus according to claim 2 wherein one well in each line is treated to form a positive control and (b) one well in each line is treated to form a negative control; the positive control well (a) including a first coating of a specific antigen, antibody or like material.
Claim 7
Dependent Claim
49 words
An apparatus for an enzyme-linked immunosorbent assay including (1) a first vessel according to claim 2, (2) at least one further vessel adjacent thereto, (3) a first dividing member between adjacent vessels, and (4) a second dividing member between the at least one coated well and the control well.
Claim 8
Dependent Claim
100 words
A method of assaying a sample which method includes (a) providing a sample, (b) providing an apparatus as defined in claim 1, (c) adding a portion of the sample to be tested to at least one coated non-control well of the first line, (d) adding a portion of the sample to the at least non-coated non-control well of the second and any subsequent lines, (e) adding an enzyme-linked species-specific antibody conjugate to the sample wells and control wells of the first and second lines, and (f) treating the product of step (e) with a substrate for the enzyme-linked antibody conjugate.
Claim 9
Dependent Claim
111 words
A method according to claim 7 wherein the sample addition steps (c) and (c) include (i) adding one drop of sample extract to each coated non-control well in each line, (ii) incubating the coated vessel for a period sufficient to allow the specific antibody in the sample to become attached to the species-specific antigen, antibody or like material; and wherein the conjugate additions to (e) includes (i) adding one drop of enzyme-linked species-specific antibody conjugate to the or each coated non-control well of each line corresponding to the species to be identified, and (ii) incubating the coated vessel for a period sufficient to allow reaction between the conjugate and captured antibody.
Claim 10
Independent Claim
107 words
The method of preparing a vessel for use in a method for assaying a sample which includes (a) providing a vessel including (i) a first line of wells, and (ii) a second line of wells, (b) coating at least one of the wells of the first line with a first species specific antigen, antibody or like material, (c) coating at least one of the wells of the second line with a second species-specific antigen, antibody or like material, (d) treating at least one well in the first line to form a control, and (e) treating at least one well in the second line to form a control.
Claim 11
Independent Claim
107 words
The method of preparing a vessel for use in a method for assaying a sample which includes (a) providing a vessel including (i) a first line of wells, and (ii) a second line of wells, (b) coating at least one of the wells of the first line with a first species specific antigen, antibody or like material, (c) coating at least one of the wells of the second line with a second species-specific antigen, antibody or like material, (d) treating at least one well in the first line to form a control, and (e) treating at least one well in the second line to form a control.
Claim 12
Dependent Claim
39 words
A method according to claim 9 wherein the coating steps (b) and (c) include (i) treating the at least one well of the first line with a first species specific antigen, antibody or like material in a buffer solution.
Claim 13
Dependent Claim
26 words
A method according to claim 10 wherein step (d) includes treating at least one well in the first line to form a positive or negative control.
Claim 14
Dependent Claim
51 words
A method according to claim 11 wherein the control is a positive control and treatment step (d) includes (i) repeating step (b) on a second well of the first line and (ii) coating the second well of the first line with a coating standard being an assay sample of known composition.
Claim 15
Dependent Claim
41 words
A test kit for assaying a sample which test kit includes (a) an apparatus for enzyme-linked immunosorbent assay according to claim 1, (b) a plurality of species-specific antibody-enzyme conjugants in suitable containers, and (c) an enzyme substrate in a suitable container.
References Cited (Backward Citations)
CH613523A5
Cited
EP0084102A1
Cited
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.