EP0176080A2
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EP
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Blood partitioning method and apparatus
Assignee: Becton Dickinson and Co
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Filing: 1985-09-24
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Granted: —
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Expires: 2005-09-24
Abstract
Abstract This inventions relates to a method of separation and partition of the mononuclear cells from a blood sample which comprises (a) providing a centrifugation tube; (b) placing within said tube a thixotropic, gel-like substance and a water soluble density gradient material having a specific gravity higher than that of said gel-like substance; (c) placing a sample of blood within said tube; (d) subjecting said tube to a centrifugal force to separate mononuclear cells from other cells in said blood sample and establishing a barrier layer between said mononuclear cells and said other cells consisting of separate layers of said gel-like substance and said water soluble density gradient material.
Inventors
- 👤 Alex M. Saunders
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 13:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/491; B; B01; B01D; B01D43/00; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
103 words
A method of separation and partition of the mononuclear cells from a blood sample comprising: (a) providing a centrifugation tube; (b) placing within said tube a thixotropic, gel-like substance and a water soluble density gradient material having a specific gravity higher than that of said gel-like substance; (c) placing a sample of blood within said tube; (d) subjecting said tube to a centrifugal force to separate mononuclear cells from other cells in said blood sample and establishing a barrier layer between said mononuclear cells and said other cells consisting of separate layers of said gel-like substance and said water soluble desnity gradient material.
Claim 2
Independent Claim
103 words
A method of separation and partition of the mononuclear cells from a blood sample comprising: (a) providing a centrifugation tube; (b) placing within said tube a thixotropic, gel-like substance and a water soluble density gradient material having a specific gravity higher than that of said gel-like substance; (c) placing a sample of blood within said tube; (d) subjecting said tube to a centrifugal force to separate mononuclear cells from other cells in said blood sample and establishing a barrier layer between said mononuclear cells and said other cells consisting of separate layers of said gel-like substance and said water soluble desnity gradient material.
Claim 3
Dependent Claim
24 words
A method in accordance with Claim 1 wherein said sample is centrifuged at a force of from about 600 g to about 2000 g.
Claim 4
Dependent Claim
23 words
A method in accordance with Claim 1 wherein said sample is centrifuged for a time of from about 5 to about 20 minutes.
Claim 5
Dependent Claim
14 words
A method in accordance with Claim 1 wherein said gel-like substance is a polyester.
Claim 6
Dependent Claim
22 words
A method in accordance with Claim 1 wherein said gel-like substance is a mixture of silicone fluid and a hydrophobic powdered silica.
Claim 7
Dependent Claim
22 words
A method in accordance with Claim 1 wherein said gel-like substance is a mixture of a hydrocarbon polymer and a powdered silica.
Claim 8
Dependent Claim
22 words
A method in accordance with Claim 1 wherein said density gradient material is an aqueous solution of a polymeric saccharide and diatriazoate.
Claim 9
Dependent Claim
35 words
A method in accordance with Claim 1 wherein said tube containing said thixotropic, gel-like sustance and said water soluble density gradient material is subjected to centrifugation prior to placing said blood sample within said tube.
Claim 10
Dependent Claim
24 words
A method in accordance with Claim 1 wherein said thixotropic, gel-like substance has a specific gravity of from about 1.07 to about 1.085 g/cc.
Claim 11
Dependent Claim
24 words
A method in accordance with Claim 1 wherein said thixotropic, gel-like substance has a specific gravity of from about 1.075 to about 1.08 g/cc.
Claim 12
Dependent Claim
24 words
A method in accordance with Claim 1 wherein said density gradient material has a specific gravity of from about 1.080 to about 1.100 g/cc.
Claim 13
Dependent Claim
24 words
A method in accordance with Claim 1 wherein said density gradient material has a specific gravity of from about 1.085 to about 1.095 g/cc.
Claim 14
Dependent Claim
31 words
A method in accordance with Claim 1 wherein said thixotropic, gel-like material has a specific gravity of 1.077 g/cc and said density gradient material has a specific gravity of 1.09 g/cc.
Claim 15
Dependent Claim
45 words
A centrifuge tube suitable for the separation and partition of mononuclear cells from a blood sample comprising a centrifuged tube having located therein a thixotropic, gel-like substance and a water soluble density gradient material having a specific gravity higher than that of said gel-like substance.
Claim 16
Dependent Claim
45 words
A centrifuge tube suitable for the separation and partition of mononuclear cells from a blood sample comprising a centrifuged tube having located therein a thixotropic, gel-like substance and a water soluble density gradient material having a specific gravity higher than that of said gel-like substance.
Claim 17
Dependent Claim
15 words
A centrifuge tube in accordance with Claim 14 wherein said gel-like substance is a polyester.
Claim 18
Dependent Claim
23 words
A centrifuge tube in accordance with Claim 14 wherein said gel-like substance is a mixture of silicone fluid and a hydrophobic powdered silica.
Claim 19
Dependent Claim
23 words
A centrifuge tube in accordance with Claim 14 wherein said gel-like substance is a mixture of a hydrocarbon polymer and a powdered silica.
Claim 20
Dependent Claim
23 words
A centrifuged tube in accordance with Claim 14 wherein said density gradient material is an aqueous solution of a polymeric saccharide and diatriazoate.
Claim 21
Dependent Claim
25 words
A centrifuge tube in accordance with Claim 14 wherein said thixotropic, gel-like substance has a specific gravity of from about 1.07 to about 1.085 g/cc.
Claim 22
Dependent Claim
25 words
A centrifuge tube in accordance with Claim 14 wherein said thixotropic, gel-like substance has a specific gravity of from about 1.075 to about 1.08 g/cc.
Claim 23
Dependent Claim
25 words
A centrifuge tube in accordance with Claim 14 wherein said density gradient material has a specific gravity of from about 1.080 to about 1.100 g/cc.
Claim 24
Dependent Claim
24 words
A centrifuge tube in accordance with Claim 14 wherein density gradient material has a specific gravity of from about 1.085 to about 1.095 g/cc.
Claim 25
Dependent Claim
32 words
A centrifuge tube in accordance with Claim 14 wherein said thixotropic, gel-like material has a specific gravity of 1.077 g/cc and said density gradient material has a specific gravity of 1.09 g/cc.
References Cited (Backward Citations)
No citation records indexed.
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