EP0171140A2
Expired / Lapsed
EP
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Automatic cycling reaction apparatus and automatic analyzing apparatus using the same
Assignee: The University of Tokyo
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Filing: 1985-05-15
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Granted: —
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Expires: 2005-05-15
Abstract
Abstract An apparatus for carrying out an enzymatic cycling reaction including a turntable (3) arranged in a reaction tank (1), a number of reaction vessels (2) being arranged in the turntable along its periphery, a device (6, 7) for circulating an antifreeze liquid through the reaction tank (1), a heater (9) for heating the antifreeze liquid, a refrigerator (10), for cooling the antifreeze liquid and a switching valve (8) for selectively passing the antifreeze liquid through the heater (9) or refrigerator (10). Until all the samples and cycling mixture are delivered into the reaction vessels, the reaction tank (1) is held at a very low temperature at which no enzymatic cycling reaction proceeds. Then the antifreeze liquid is heated to an enzymatic cycling reaction temperature so as to perform the enzymatic cycling reaction simultaneously for liquids contained in all the reaction vessels for a desired period. Then, the antifreeze liquid is heated to a high temperature so as to stop simultaneously the cycling reaction in all the reaction vessels.
Inventors
- 👤 Yoshiyuki Suzuki
- 👤 Takahiko Kato
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 08:57
CPC Classifications
B; B01; B01L; B01L7/00; B01L7/52; C; C12; C12M; C12M21/00; C12M21/18; C12M41/00; C12M41/30; C12M41/36; G; G01; G01N; G01N35/00; G01N35/02; G01N2035/00346; G01N2035/00356; G01N2035/00366; G01N2035/00386; G01N2035/00396; G01N2035/00435; G01N2035/00445; G01N35/04; G01N2035/0401; G01N2035/0418; G01N2035/042; G01N35/10; G01N2035/1027; G01N2035/1048; G01N2035/1058; G01N2035/106; G01N35/025; G01N35/1004
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
91 words
An automatic enzymatic cycling reaction apparatus comprising first means for supporting a plurality of reaction vessels each containing given amounts of a sample and a cycling mixture including enzymes; and second means for holding simultaneously liquids contained in all the reaction vessels at a given cycling reaction temperature for a given period, holding all the liquids simultaneously at a first temperature at which a cycling reaction is stopped due to loss of activity of enzymes, and then keeping all the liquids simultaneously at a second temperature lower than the first temperature.
Claim 2
Dependent Claim
91 words
An automatic enzymatic cycling reaction apparatus comprising first means for supporting a plurality of reaction vessels each containing given amounts of a sample and a cycling mixture including enzymes; and second means for holding simultaneously liquids contained in all the reaction vessels at a given cycling reaction temperature for a given period, holding all the liquids simultaneously at a first temperature at which a cycling reaction is stopped due to loss of activity of enzymes, and then keeping all the liquids simultaneously at a second temperature lower than the first temperature.
Claim 3
Dependent Claim
58 words
An apparatus according to claim 1, wherein said first means comprises a turntable which holds a number of reaction vessels along its periphery, and said second means comprises a reaction tank in which said turntable is arranged, a device for circulating a thermostatic liquid through said tank and a device for controlling a temperature of the thermostatic liquid.
Claim 4
Dependent Claim
38 words
An apparatus according to claim 2, wherein said device for controlling the temperature of the thermostatic liquid comprises a heater and a refrigerator and a switching valve for selectively passing the thermostatic liquid through the heater or refrigerator.
Claim 5
Dependent Claim
20 words
An apparatus according to claim 3, wherein said second means further comprises a lid removably placed on the reaction tank.
Claim 6
Dependent Claim
53 words
An apparatus according to claim 1, wherein said first means comprises a rack in which a number of reaction vessels are set and a device for transporting the rack, and said second means comprises a plurality of tanks into which said rack is selectively inserted and whose temperatures are kept at given temperatures.
Claim 7
Dependent Claim
58 words
An apparatus according to claim 5, wherein there are arranged four tanks, a first tank is held at a low temperature at which no enzymatic cycling reaction proceeds, a second tank is kept at the enzymatic cycling reaction temperature, a third tank is held at the first temperature and a fourth tank is kept at the second temperature.
Claim 8
Dependent Claim
151 words
An automatic analyzer using an enzymatic cycling reaction comprising first means for supporting a number of reaction vessels each including given amounts of a sample and a cycling mixture including enzymes; second means for keeping simultaneously liquids contained in all the reaction vessels at a first temperature at which an enzymatic cycling reaction proceeds for a given period, holding all the liquids simultaneously at a second temperature at which the enzymatic cycling reaction is stopped due to loss of activity of the enzymes, and keeping all the liquids simultaneously at a third temperature lower than the second temperature and at which temperature an indicator reaction proceeds; third means for delivering a given amount of an indicator reagent into respective reaction vessels after the enzymatic cycling reaction of the liquids in the reaction vessels has been stopped; and fourth means for measuring fluorescent light emitted from substances produced by the indicator reaction.
Claim 9
Dependent Claim
151 words
An automatic analyzer using an enzymatic cycling reaction comprising first means for supporting a number of reaction vessels each including given amounts of a sample and a cycling mixture including enzymes; second means for keeping simultaneously liquids contained in all the reaction vessels at a first temperature at which an enzymatic cycling reaction proceeds for a given period, holding all the liquids simultaneously at a second temperature at which the enzymatic cycling reaction is stopped due to loss of activity of the enzymes, and keeping all the liquids simultaneously at a third temperature lower than the second temperature and at which temperature an indicator reaction proceeds; third means for delivering a given amount of an indicator reagent into respective reaction vessels after the enzymatic cycling reaction of the liquids in the reaction vessels has been stopped; and fourth means for measuring fluorescent light emitted from substances produced by the indicator reaction.
Claim 10
Dependent Claim
57 words
An analyzer according to claim 7, wherein said first means comprises a turntable for supporting the reaction vessels along its periphery, and said second means comprises a reaction tank in which said turntable is arranged, a device for circulating a thermostatic liquid through the reaction tank, and a device for controlling the temperature of the thermostatic liquid.
Claim 11
Dependent Claim
31 words
An analyzer according to claim 8, wherein said temperature controlling device comprises a heater, a refrigerator and a switching valve for selectively passing the thermostatic liquid through the heater or refrigerator.
Claim 12
Dependent Claim
20 words
An analyzer according to claim 9, wherein said second means further comprises a lid removably placed on the reaction tank.
Claim 13
Dependent Claim
53 words
An analyzer according to claim 7, wherein said first means comprises a rack in which a number of reaction vessels are set and a device for transporting the rack, and said second means comprises a plurality of tanks into which said rack is selectively inserted and whose temperatures are kept at given temperatures.
Claim 14
Dependent Claim
81 words
An analyzer according to claim 11, wherein there are arranged five tanks, a first tank is held at a low temperature at which no enzymatic cycling reaction proceeds, a second tank is kept at the enzymatic cycling reaction temperature, a third tank is held at the first temperature, a fourth tank is kept at a low temperature at which the indicator reaction does not proceed, and a fifth tank is held at the second temperature at which the indicator reaction proceeds.
Claim 15
Dependent Claim
32 words
An analyzer according to claim 12, wherein said second means further comprises a station in which the rack is transferred from the fifth tank, said station being held at the room temperature.
Claim 16
Dependent Claim
58 words
An analyzer according to claim 13, wherein said first to fifth tanks and station are arranged circularly about a center axis and said device for ,transporting the rack comprises an arm for holding the rack and a shaft arranged in said central axis rotatably as well as movably up and down, said arm being secured to the shaft.
Claim 17
Dependent Claim
72 words
An analyzer according to claim 14, wherein said third means for delivering the indicator reagent is arranged above the fourth tank and comprises a frame member movably arranged between a first position above the fourth tank and a second position away from the fourth tank, and at least one nozzle movably secured to the frame member so as to deliver the indicator reagent into the reaction vessels positioned at the fourth tank.
Claim 18
Dependent Claim
34 words
An analyzer according to claim 8, further comprising a sampler for holding a number of samples to be analyzed and a device for delivering a given amount of each samples into respective reaction vessels.
References Cited (Backward Citations)
No citation records indexed.
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