EP0170446A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Methods of assay
Assignee: F Hoffmann La Roche AG
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Filing: 1985-07-09
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Granted: —
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Expires: 2005-07-09
Abstract
Abstract In its broadest aspect, the present invention provides a method of effecting an electrochemical specific-binding assay of a ligand, either qualitatively or quantitatively, in an apparatus comprising at least one electrode, in which method a labelled component of the assay medium is, at least in part, magnetically held in the vicinity of the electrode. The electrochemical assay method may include a separation step, whereby bound label may be separated from free label in the assay medium. Preferably the electrochemical assay method will include the step of determining a perturbation in an electrochemical characteristic of components of the assay medium associated with a ligand complexing reaction. Such an assay method is applicable to, for example, antibodies and antigens and suitable labels include redox centres, enzyme labels in the presence of an electron-transfer mediator and electron-transfer mediator Jabals in the presence of an electron donor or acceptor.
Inventors
- 👤 Simon John Rattle
- 👤 Gordon Coulter Forrest
- 👤 Grenville Arthur Robinson
- 👤 Hugh Allen Oliver Hill
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 17:14
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/543; G01N33/54313; G01N33/54326; G01N33/54366; G01N33/54373; G01N33/5438; Y; Y10; Y10S; Y10S204/00; Y10S204/05; Y10S435/00; Y10S435/817; Y10S436/00; Y10S436/806
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
45 words
A method of effecting an electrochemical specific-binding assay of a ligand, either qualitatively or quantitatively, in an apparatus comprising at least one electrode, in which method a labelled component of the assay medium is, at least in part, held in the vicinity of the electrode.
Claim 2
Independent Claim
45 words
A method of effecting an electrochemical specific-binding assay of a ligand, either qualitatively or quantitatively, in an apparatus comprising at least one electrode, in which method a labelled component of the assay medium is, at least in part, held in the vicinity of the electrode.
Claim 3
Dependent Claim
25 words
A method as claimed in claim 1 which includes a separation step, whereby bound label may be separated from free label in the assay medium.
Claim 4
Dependent Claim
31 words
A method as claimed in claim 1 which includes the step of determining a perturbation in an electrochemical characteristic of components of the assay medium associated with a ligand complexing reaction.
Claim 5
Independent Claim
43 words
A method as claimed in any one of claims 1 to 3 wherein a redox centre label, an enzyme label in the presence of an electron-transfer mediator or an electron-transfer mediator label in the presence of an electron donor or acceptor is employed.
Claim 6
Dependent Claim
15 words
A method as claimed in claim 4 wherein ferrocene or a derivative thereof is employed.
Claim 7
Independent Claim
47 words
A method as claimed in any one of the preceding claims wherein the labelled component of the assay medium is retained in the vicinity of the working electrode by immobilising at least some of the labelled component on a magnetic support and employing a magnetic working electrode.
Claim 8
Independent Claim
19 words
A method as claimed in any one of the preceding claims wherein the ligand is an antibody or antigen.
Claim 9
Dependent Claim
16 words
A magnetic electrode-for use in a method as claimed in any one of the preceding claims.
Claim 10
Dependent Claim
29 words
An electrode suitable for use in a method as claimed in claim 1 which comprises an elongate magnetic or magnetisable core terminally capped or tipped with an electrode surface.
Claim 11
Dependent Claim
17 words
An electrode as claimed in claim 9 wherein the electrode surface is magnetically screened from the core.
Claim 12
Dependent Claim
23 words
A kit of reagents and/or apparatus for carrying out a method of assay as claimed in any one of claims 1 to 7.
Claim 13
Dependent Claim
43 words
A kit as claimed in claim 11 which comprises an electrochemical apparatus containing at least two electrodes and/or an aqueous assay mediium with suitable components present, including a labelled component, the said labelled component being in solution or immobilised on a magnetic support.
Claim 14
Independent Claim
46 words
A method of effecting an electrochemical specific-binding assay of a liqand, either qualitatively or quantitatively, in an apparatus comprising at least one electrode, in which method a labelled component of the assay medium is, at least in part, maqnetically held in the vicinity of the electrode.
Claim 15
Independent Claim
46 words
A method of effecting an electrochemical specific-binding assay of a liqand, either qualitatively or quantitatively, in an apparatus comprising at least one electrode, in which method a labelled component of the assay medium is, at least in part, maqnetically held in the vicinity of the electrode.
Claim 16
Dependent Claim
25 words
A method as claimed in claim 1 which includes a separation step, whereby bound label may be separated from free label in the assay medium.
Claim 17
Dependent Claim
31 words
A method as claimed in claim 1 which includes the step of determining a perturbation in an electrochemical characteristic of components of the assay medium associated with a ligand complexing reaction.
Claim 18
Independent Claim
43 words
A method as claimed in any one of claims 1 to 3 wherein a redox centre label, an enzyme label in the presence of an electron-transfer mediator or an electron-transfer mediator label in the presence of an electron donor or acceptor is employed.
Claim 19
Dependent Claim
15 words
A method as claimed in claim 4 wherein ferrocene or a derivative thereof is emploved.
Claim 20
Independent Claim
47 words
A method as claimed in any one of the preceding claims wherein the labelled component of the assay medium is retained in the vicinity of the working electrode by immobilising at least some of the labelled component on a magnetic support and employing a magnetic working electrode.
Claim 21
Independent Claim
19 words
A method as claimed in any one of the preceding claims wherein the ligand is an antibody or antigen.
References Cited (Backward Citations)
FR2408142A1
Cited
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