EP0167443A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Coccidiosis vaccine
Assignee: Merck and Co Inc
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Filing: 1985-06-21
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Granted: —
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Expires: 2005-06-21
Abstract
Abstract Sporozoites of coccidia fail to develop in chickens which are immune and many are blocked from penetrating host cells. Although previous attempts to immunize chickens with non-viable coccidial antigens have been unsuccessful it has been discovered that extracts from sporozoites or sporulated oocysts of E. tenella induce high levels of protective immunity. These extracts contain at least 15 polypeptides many of which are associated with the surface of the sporozoite and induce good immune responses. Antibody to these polypeptides blocks sporozoite-host cell penetration in vitro and neutralizes sporozoites in vivo. One or more of these polypeptides may be used as an antigen to protect against coccidiosis.
Inventors
- 👤 Peter K. Murray
- 👤 Stefan Galuska
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 03:44
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/44; C07K14/455; A; A61; A61K; A61K39/00; A61K39/002; A61K39/012
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
35 words
An extract obtained by grinding and centrifuging a suspension of sporulated oocysts of Eimeria tenella, and collecting the supernatant liquid resulting from the centrifugation step, the extract containing antigens capable of immunizing chickens against coccidiosis.
Claim 2
Dependent Claim
35 words
An extract obtained by grinding and centrifuging a suspension of sporulated oocysts of Eimeria tenella, and collecting the supernatant liquid resulting from the centrifugation step, the extract containing antigens capable of immunizing chickens against coccidiosis.
Claim 3
Dependent Claim
38 words
An extract obtained by grinding and centrifuging a suspension of sporulated oocysts of Eimeria tenella, and purifying sporozoites from the pelleted material resulting from the centrifugation step, the pelleted material containing antigens capable of immunizing chickens against coccidiosis.
Claim 4
Dependent Claim
38 words
An extract obtained by grinding and centrifuging a suspension of sporulated oocysts of Eimeria tenella, and purifying sporozoites from the pelleted material resulting from the centrifugation step, the pelleted material containing antigens capable of immunizing chickens against coccidiosis.
Claim 5
Dependent Claim
22 words
Immunogenic polypeptides of the supernatant liquid of Claim 1 having molecular weights of 235, 105, 94, 71, 64, 45, and 26 KD.
Claim 6
Dependent Claim
24 words
Immunogenic polypeptides of the pelleted material of Claim 2 having molecular weights of 235, 105, 94, 82, 68, 45, 40, 26, and 23 KD.
Claim 7
Dependent Claim
33 words
Immunogenic polypeptides of the pelleted material of Claim 2 having molecular weights of <10, 10, 15, 19, 26, 28, 45, 50, 68, 82, 94, 105 and 330 KD against which monoclonal antibodies react.
Claim 8
Dependent Claim
20 words
A method of immunizing chickens against coccidiosis comprising administering an anti- coccidial effective dose of the extract of Claim 1.
Claim 9
Dependent Claim
20 words
A method of immunizing chickens against coccidiosis comprising administering an anti- coccidial effective dose of the extract of Claim 2.
Claim 10
Dependent Claim
21 words
A method of immunizing chickens against coccidiosis comprising administering an anti- coccidial effective dose of the immunogenic polypeptides of Claim 3.
Claim 11
Dependent Claim
21 words
A method of immunizing chickens against coccidiosis comprising administering an anti- coccidial effective dose of the immunogenic polypeptides of Claim 4.
Claim 12
Dependent Claim
21 words
A method of immunizing chickens against coccidiosis comprising administering an anti- coccidial effective dose of the immunogenic polypeptides of Claim 5.
Claim 13
Dependent Claim
13 words
A composition comprising the extract of Claim 1 in a physiologically acceptable medium.
Claim 14
Dependent Claim
13 words
A composition comprising the extract of Claim 2 in a physiologically acceptable medium.
Claim 15
Dependent Claim
14 words
A composition comprising the immunogenic polypeptides of Claim 3 in a physiologically acceptable medium.
Claim 16
Dependent Claim
14 words
A composition comprising the immunogenic polypeptides of Claim 4 in a physiologically acceptable medium.
Claim 17
Dependent Claim
14 words
A composition comprising the immunogenic polypeptides of Claim 5 in a physiologically acceptable medium.
References Cited (Backward Citations)
No citation records indexed.
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