EP0164176A2
Expired / Lapsed
EP
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Antigenic proteins and vaccines containing them and protective antibodies directed to them for prevention of coccidiosis caused by Eimeria tenella
Assignee: Dimminaco AG
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Filing: 1985-06-05
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Granted: —
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Expires: 2005-06-05
Abstract
Abstract A purified antigenic protein has been obtained which is capable of inducing in a chicken an immune response conferring protection against infection by Eimeria tenella or Eimeria necatrix. The protein has a molecular weight of about 25,000 and is composed of two polypeptides joined by a disulfide bond. The two polypeptide subunits have molecular weights of about 17,000 and about 8,000, respectively. The gene encoding the protein has been sequenced and the amino acid sequence of the protein deduced therefrom and by direct peptide sequencing. The protein and antigenic polypeptides having an amino acid sequence included within the protein may be incorporated into a vaccine for conferring upon a chicken active immunity against infection by E. tenella and E. necatrix. A hybridoma cell line (ATCC No. HB8561) has been developed which produces a monoclonal antibody designated Ptn 7.2A4/4. This antibody may be used to confer upon a chicken specific passive protection against infection by E. tenella and E. necatrix. The antibody may also be used to obtain the purified protein antigen and the 11,500 and 6,500 dalton polypeptide fragments thereof. Finally, an anti-idiotype antibody to the Ptn. 7.2A4/4 monoclonal antibody may be prepared and used to confer upon a chicken active immunity against E. tenella and E. necatrix infection.
Inventors
- 👤 Jr. Karel Z. Newman
- 👤 John L. Tedesco
- 👤 Randy R. Simonson
- 👤 Thomas C. Gore
- 👤 Gary R. Petersen
- 👤 Virginia Mary Brothers
- 👤 James Gordon Files
- 👤 Leland Shawn Paul
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 20:26
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/42; C07K16/4208; C07K16/4241; C07K16/425; C07K14/00; C07K14/435; C07K14/44; C07K14/455; C07K16/18; C07K16/20; A; A61; A61K; A61K38/00; A61K39/00
Claims Analysis
Total Claims: 174
Claim 1
Independent Claim
18 words
Ali, N.S., Binnerts, W.T. and Klimes, B. (1972). Immunization by (sic) irradiated Eimeria acervulina. J. Prot. 19, 177.
Claim 2
Dependent Claim
18 words
Ali, N.S., Binnerts, W.T. and Klimes, B. (1972). Immunization by (sic) irradiated Eimeria acervulina. J. Prot. 19, 177.
Claim 3
Dependent Claim
20 words
Benton, W.D. and Davis, R.W. (1977). Screening Àgt recombinant clones by hybridization to single plaques in situ. Science 196, 180-182.
Claim 4
Dependent Claim
20 words
Benton, W.D. and Davis, R.W. (1977). Screening Àgt recombinant clones by hybridization to single plaques in situ. Science 196, 180-182.
Claim 5
Dependent Claim
33 words
Blobel, G. and Dobberstein, B. (1975). Transfer of proteins across membranes I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J. Cell Biol. 67, 835-851.
Claim 6
Dependent Claim
33 words
Blobel, G. and Dobberstein, B. (1975). Transfer of proteins across membranes I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J. Cell Biol. 67, 835-851.
Claim 7
Dependent Claim
32 words
Burnette, W.M. (1981). "Western Blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate - polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112, 195.
Claim 8
Dependent Claim
32 words
Burnette, W.M. (1981). "Western Blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate - polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112, 195.
Claim 9
Dependent Claim
22 words
Cohen, S.A., Tarvin, T.L. and Bidlingmeyer, B.A. (1984). Analysis of amino acids using precolumn deri- vatization with phenylisothiocyanate. American Laboratory 16, 48-59.
Claim 10
Dependent Claim
22 words
Cohen, S.A., Tarvin, T.L. and Bidlingmeyer, B.A. (1984). Analysis of amino acids using precolumn deri- vatization with phenylisothiocyanate. American Laboratory 16, 48-59.
Claim 11
Dependent Claim
18 words
Danforth, H.D. (1982). Development of hybridoma-produced antibodies directed against Eimeria tenella and E. mitis. J. Parasitol. 68, 392.
Claim 12
Dependent Claim
18 words
Danforth, H.D. (1982). Development of hybridoma-produced antibodies directed against Eimeria tenella and E. mitis. J. Parasitol. 68, 392.
Claim 13
Dependent Claim
31 words
Danforth, H.D. (1983). Use of monoclonal antibodies directed against Eimeria tenella sporozoites to determine stage specificity and in vitro effect on parasite penetration and development. Am J. Vet. Res 44, 1722.
Claim 14
Dependent Claim
31 words
Danforth, H.D. (1983). Use of monoclonal antibodies directed against Eimeria tenella sporozoites to determine stage specificity and in vitro effect on parasite penetration and development. Am J. Vet. Res 44, 1722.
Claim 15
Dependent Claim
25 words
Danforth, H.D. and Augustine P.C. (1983). Specificity and cross-reactivity of immune serum and hybridoma antibodies to various species of avian coccidia. Poultry Science 62, 2145.
Claim 16
Dependent Claim
25 words
Danforth, H.D. and Augustine P.C. (1983). Specificity and cross-reactivity of immune serum and hybridoma antibodies to various species of avian coccidia. Poultry Science 62, 2145.
Claim 17
Dependent Claim
22 words
Davis, P.J., Parry, S.H. and Porter, P. (1978). The role of secretory IgA in anti-coccidial immunity in the chicken. Immunology 34, 879.
Claim 18
Dependent Claim
22 words
Davis, P.J., Parry, S.H. and Porter, P. (1978). The role of secretory IgA in anti-coccidial immunity in the chicken. Immunology 34, 879.
Claim 19
Dependent Claim
26 words
Davis, P.J. and Porter, P. (1979). A mechanism for secretory IgA mediated inhibition of the cell penetration and intracellular development of Eimeria tenella. Immunology 36, 471.
Claim 20
Dependent Claim
26 words
Davis, P.J. and Porter, P. (1979). A mechanism for secretory IgA mediated inhibition of the cell penetration and intracellular development of Eimeria tenella. Immunology 36, 471.
Claim 21
Dependent Claim
19 words
Dvorak, J.A. and Crane, M. St. J. (1981). Vertebrate cell cycle modulates infection by protozoan parasites. Science 214, 1034.
Claim 22
Dependent Claim
19 words
Dvorak, J.A. and Crane, M. St. J. (1981). Vertebrate cell cycle modulates infection by protozoan parasites. Science 214, 1034.
Claim 23
Dependent Claim
19 words
Edman, P. and Begg, G. (1967). A protein sequenator. Automated Equipment for Sequence determination, Eur. J. Biochem 1, 80.
Claim 24
Dependent Claim
19 words
Edman, P. and Begg, G. (1967). A protein sequenator. Automated Equipment for Sequence determination, Eur. J. Biochem 1, 80.
Claim 25
Dependent Claim
20 words
Giambrone, J.J., Klesius, P.H. and Edgar, S.A. (1980). Avian Coccidiosis: Evidence for a cell-mediated immune response. Poultry Sci. 59, 38.
Claim 26
Dependent Claim
20 words
Giambrone, J.J., Klesius, P.H. and Edgar, S.A. (1980). Avian Coccidiosis: Evidence for a cell-mediated immune response. Poultry Sci. 59, 38.
Claim 27
Dependent Claim
31 words
Gibbons, R.A., Sellwood, R., Burrow, M. and Hunter, P.A. (1977). Inheritance of resistance to neonatal E. coli diarrhea in the pig: examination of the genetic system. Theor. Appl. Genet. 51, 65.
Claim 28
Dependent Claim
31 words
Gibbons, R.A., Sellwood, R., Burrow, M. and Hunter, P.A. (1977). Inheritance of resistance to neonatal E. coli diarrhea in the pig: examination of the genetic system. Theor. Appl. Genet. 51, 65.
Claim 29
Dependent Claim
28 words
Gore, T.C., Long, P.L., Kogut, M. and Johnson, J. (1983). Attenuation of Eimeria necatrix and E. tenella of U.S. origin by serial embryo passage. Avian Disease 27, 569.
Claim 30
Dependent Claim
28 words
Gore, T.C., Long, P.L., Kogut, M. and Johnson, J. (1983). Attenuation of Eimeria necatrix and E. tenella of U.S. origin by serial embryo passage. Avian Disease 27, 569.
Claim 31
Dependent Claim
29 words
Hunkapiller, M.W., Hewick, R.M., Dreyer, W.J. and Hood, L.E. (1983). High sensitivity sequencing with a gas phase sequenator. Methods in Enzymology 91, A ca- demic Press, New York, 399-413.
Claim 32
Dependent Claim
29 words
Hunkapiller, M.W., Hewick, R.M., Dreyer, W.J. and Hood, L.E. (1983). High sensitivity sequencing with a gas phase sequenator. Methods in Enzymology 91, A ca- demic Press, New York, 399-413.
Claim 33
Dependent Claim
17 words
Hunkapiller, M.W., Strickler, J.E. and Wilson, K.J. (1984). Contemporary Methodology for Protein Structure Determination. Science 226, 304-311.
Claim 34
Dependent Claim
17 words
Hunkapiller, M.W., Strickler, J.E. and Wilson, K.J. (1984). Contemporary Methodology for Protein Structure Determination. Science 226, 304-311.
Claim 35
Dependent Claim
15 words
Jeffers, T.K. (1975). Attenuation of Eimeria tenella through selection for precociousness. J. Parasitol. 61, 1083.
Claim 36
Dependent Claim
15 words
Jeffers, T.K. (1975). Attenuation of Eimeria tenella through selection for precociousness. J. Parasitol. 61, 1083.
Claim 37
Dependent Claim
19 words
Jeffers, T.K. (1976). Genetic recombination of precociousness and anticoccidial drug resistance on Eimeria tenella. Zeitschrift fur Parasitenkunde 50, 251.
Claim 38
Dependent Claim
19 words
Jeffers, T.K. (1976). Genetic recombination of precociousness and anticoccidial drug resistance on Eimeria tenella. Zeitschrift fur Parasitenkunde 50, 251.
Claim 39
Dependent Claim
23 words
Johnson, J. and Reid, W.M. (1970). Anticoccidial Drugs: Lesion scoring techniques in battery and floor pen experiments with chickens. Exp. Parasitology 38, 36.
Claim 40
Dependent Claim
23 words
Johnson, J. and Reid, W.M. (1970). Anticoccidial Drugs: Lesion scoring techniques in battery and floor pen experiments with chickens. Exp. Parasitology 38, 36.
Claim 41
Dependent Claim
27 words
Kasper, L.H., Crabb, J.H., and Pfefferkorn, E.R. (1983). Purification of a major membrane protein of Toxoplasma gondii by immunoabsorption with a monoclonal antibody. J. Immunol. 130, 2407.
Claim 42
Dependent Claim
27 words
Kasper, L.H., Crabb, J.H., and Pfefferkorn, E.R. (1983). Purification of a major membrane protein of Toxoplasma gondii by immunoabsorption with a monoclonal antibody. J. Immunol. 130, 2407.
Claim 43
Dependent Claim
18 words
Keusch, G.T. (1979). Specific membrane receptors: Pathogenic and therapeutic implications in infectious diseases. Rev. Inf. Dis. 1, 517.
Claim 44
Dependent Claim
18 words
Keusch, G.T. (1979). Specific membrane receptors: Pathogenic and therapeutic implications in infectious diseases. Rev. Inf. Dis. 1, 517.
Claim 45
Dependent Claim
13 words
Kriel, G. (1981). Transport of proteins across membranes. Ann. Rev. Biochem. 50, 317-348.
Claim 46
Dependent Claim
13 words
Kriel, G. (1981). Transport of proteins across membranes. Ann. Rev. Biochem. 50, 317-348.
Claim 47
Dependent Claim
19 words
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680.
Claim 48
Dependent Claim
19 words
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680.
Claim 49
Dependent Claim
28 words
Leder, P., Tiemeier, D. and Enquist, L. (1977). EK2 derivatives of bacteriophage lambda useful in cloning of DNA from higher organisms: the λ gtWES system. Science 196, 175-177.
Claim 50
Dependent Claim
28 words
Leder, P., Tiemeier, D. and Enquist, L. (1977). EK2 derivatives of bacteriophage lambda useful in cloning of DNA from higher organisms: the λ gtWES system. Science 196, 175-177.
Claim 51
Dependent Claim
24 words
Long, P.L. (1972) Eimeria mivati: Reproduction, pathogenicity and immunogenicity of a strain maintained in chick embryos by serial passage. J. Comp. Pathol. 82, 839.
Claim 52
Dependent Claim
24 words
Long, P.L. (1972) Eimeria mivati: Reproduction, pathogenicity and immunogenicity of a strain maintained in chick embryos by serial passage. J. Comp. Pathol. 82, 839.
Claim 53
Dependent Claim
22 words
Long, P.L. (1974). Further studies on the pathogenicity and immunogenicity of an embryo adapted strain of Eimeria tenella. Avian Pathology 3, 255.
Claim 54
Dependent Claim
22 words
Long, P.L. (1974). Further studies on the pathogenicity and immunogenicity of an embryo adapted strain of Eimeria tenella. Avian Pathology 3, 255.
Claim 55
Dependent Claim
14 words
Long, P.L. (1982). The Biology of the Coccidia. University Park Press, Baltimore. Pg. 44.
Claim 56
Dependent Claim
14 words
Long, P.L. (1982). The Biology of the Coccidia. University Park Press, Baltimore. Pg. 44.
Claim 57
Dependent Claim
28 words
Long, P.L., Johnson, J., and Gore, T.C. (1982). At- . tenuation of a strain of Eimeria mivati of U.S. origin by serial embryo passage. Avian Diseases. 26, 305.
Claim 58
Dependent Claim
28 words
Long, P.L., Johnson, J., and Gore, T.C. (1982). At- . tenuation of a strain of Eimeria mivati of U.S. origin by serial embryo passage. Avian Diseases. 26, 305.
Claim 59
Dependent Claim
22 words
Long, P.L. and Rose, M.E. (1965). Active and passive immunization of chickens against induced infections of Eimeria tenella. Exp. Parasit. 16, 1.
Claim 60
Dependent Claim
22 words
Long, P.L. and Rose, M.E. (1965). Active and passive immunization of chickens against induced infections of Eimeria tenella. Exp. Parasit. 16, 1.
Claim 61
Dependent Claim
18 words
Lowder, L.J. (1966). Artificial acquired immunity to Eimeria bovis infections in cattle. Proc. Int. Congr. Parasit. 1, 106.
Claim 62
Dependent Claim
18 words
Lowder, L.J. (1966). Artificial acquired immunity to Eimeria bovis infections in cattle. Proc. Int. Congr. Parasit. 1, 106.
Claim 63
Dependent Claim
20 words
Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982). Molecular Cloning - A Laboratory Manual, Cold Springs Harbor Laboratory, New York.
Claim 64
Dependent Claim
20 words
Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982). Molecular Cloning - A Laboratory Manual, Cold Springs Harbor Laboratory, New York.
Claim 65
Dependent Claim
16 words
Marquardt, W.C. (1980). Host and site specificity in the coccidia; a perspective. J. Protozool. 26, 243.
Claim 66
Dependent Claim
16 words
Marquardt, W.C. (1980). Host and site specificity in the coccidia; a perspective. J. Protozool. 26, 243.
Claim 67
Dependent Claim
28 words
Maxam, A. and Gilbert, W. (1980). Sequencing end- labelled DNA with base-specific chemical change in: Methods in Enzymology, Vol. 65, part 1, Academic Press, New York, pp. 499-559.
Claim 68
Dependent Claim
28 words
Maxam, A. and Gilbert, W. (1980). Sequencing end- labelled DNA with base-specific chemical change in: Methods in Enzymology, Vol. 65, part 1, Academic Press, New York, pp. 499-559.
Claim 69
Dependent Claim
20 words
McDonald, V. and Ballingall, S. (1983). Attenuation of Eimeria mivati (: mitis) by selection for precocious development. Parasitology 86, 371.
Claim 70
Dependent Claim
20 words
McDonald, V. and Ballingall, S. (1983). Attenuation of Eimeria mivati (: mitis) by selection for precocious development. Parasitology 86, 371.
Claim 71
Dependent Claim
21 words
McDonald, V. and Ballingall, S. (1982). Further investigation of the pathogenicity, immunogenicity and stability of precocious Eimeria acervulina. Parasitology 86, 361.
Claim 72
Dependent Claim
21 words
McDonald, V. and Ballingall, S. (1982). Further investigation of the pathogenicity, immunogenicity and stability of precocious Eimeria acervulina. Parasitology 86, 361.
Claim 73
Dependent Claim
30 words
McDonald, V., Ballingall, S. and Shirley, M.W. (1982). A preliminary study of the nature of infection and immunity in chickens given an attenuated line of Eimeria acervulina. Parasitology 84, 21.
Claim 74
Dependent Claim
30 words
McDonald, V., Ballingall, S. and Shirley, M.W. (1982). A preliminary study of the nature of infection and immunity in chickens given an attenuated line of Eimeria acervulina. Parasitology 84, 21.
Claim 75
Dependent Claim
13 words
McDougald, L.R. Status of coccidiosis: new products on way. Poult. Digest. Oct., 1981.
Claim 76
Dependent Claim
13 words
McDougald, L.R. Status of coccidiosis: new products on way. Poult. Digest. Oct., 1981.
Claim 77
Dependent Claim
16 words
McDougald, L.R. New anticoccidial drugs: Better things to come or "endangered species?" Feedstuffs Aug. 15, 1983.
Claim 78
Dependent Claim
16 words
McDougald, L.R. New anticoccidial drugs: Better things to come or "endangered species?" Feedstuffs Aug. 15, 1983.
Claim 79
Dependent Claim
25 words
Miller, L.H., Mason, S.J., Dvorak, J.A., McGinniss, M.H., and Rothman, I.K. (1975) Erythrocyte receptors of (Plasmodium knowlesi) Malaria: Duffy blood group determinants. Science 189, 561.
Claim 80
Dependent Claim
25 words
Miller, L.H., Mason, S.J., Dvorak, J.A., McGinniss, M.H., and Rothman, I.K. (1975) Erythrocyte receptors of (Plasmodium knowlesi) Malaria: Duffy blood group determinants. Science 189, 561.
Claim 81
Dependent Claim
19 words
Reid, W.M. (1978). Protozoa. In: Diseases of Poultry. 7th ed. M.S. Hofstad, ed. Iowa State Univ. Press. pp. 942-1054.
Claim 82
Dependent Claim
19 words
Reid, W.M. (1978). Protozoa. In: Diseases of Poultry. 7th ed. M.S. Hofstad, ed. Iowa State Univ. Press. pp. 942-1054.
Claim 83
Dependent Claim
15 words
Riley, J.F. (1980). Screening for and evaluation of anticoccidial activity. Adv. Pharm. Chemo. 17, 1.
Claim 84
Dependent Claim
15 words
Riley, J.F. (1980). Screening for and evaluation of anticoccidial activity. Adv. Pharm. Chemo. 17, 1.
Claim 85
Dependent Claim
20 words
Rose, M.E. (1974). Immune responses to the Eimeria: Recent observations. Sympo. Coccidia and Related Organisms. pp. 92-118. Univ. Guelph, Ontario.
Claim 86
Dependent Claim
20 words
Rose, M.E. (1974). Immune responses to the Eimeria: Recent observations. Sympo. Coccidia and Related Organisms. pp. 92-118. Univ. Guelph, Ontario.
Claim 87
Dependent Claim
19 words
Sanger, F. and Coulson, A.R. (1978). The use of thin polyacrylamide gels for DNA sequencing. FEBS Lett. 87, 107-110.
Claim 88
Dependent Claim
19 words
Sanger, F. and Coulson, A.R. (1978). The use of thin polyacrylamide gels for DNA sequencing. FEBS Lett. 87, 107-110.
Claim 89
Dependent Claim
15 words
Schmidt, G.O. and Roberts, L.S. (1977). Foundations of Parasitology. Mosby Co., St. Louis. pp. 122-128.
Claim 90
Dependent Claim
15 words
Schmidt, G.O. and Roberts, L.S. (1977). Foundations of Parasitology. Mosby Co., St. Louis. pp. 122-128.
Claim 91
Dependent Claim
32 words
Sharma, S.D., Mullenax, J., Araujo, F.G., Erlich, H.A. and Remington, J.S. (1983). Western blot analysis of the antigens of Toxoplasma gondii recognized by human IgM and IgG antibodies. J. Immunology 131, 977.
Claim 92
Dependent Claim
32 words
Sharma, S.D., Mullenax, J., Araujo, F.G., Erlich, H.A. and Remington, J.S. (1983). Western blot analysis of the antigens of Toxoplasma gondii recognized by human IgM and IgG antibodies. J. Immunology 131, 977.
Claim 93
Dependent Claim
10 words
Sharp, P.A. (1981). Speculations on RNA splicing. Cell 23, 643-646.
Claim 94
Dependent Claim
10 words
Sharp, P.A. (1981). Speculations on RNA splicing. Cell 23, 643-646.
Claim 95
Dependent Claim
17 words
Shirley, M.W. (1980) Eimeria necatrix: The development and characteristics of an egg-adapted (attenuated) line. Parasitology 81, 525.
Claim 96
Dependent Claim
17 words
Shirley, M.W. (1980) Eimeria necatrix: The development and characteristics of an egg-adapted (attenuated) line. Parasitology 81, 525.
Claim 97
Dependent Claim
21 words
Shirley, M.W. (1982). Features of an attenuated line of Eimeria praecox. Parasitology. Proceedings of the British Soc. for Parasitology 81, 525.
Claim 98
Dependent Claim
21 words
Shirley, M.W. (1982). Features of an attenuated line of Eimeria praecox. Parasitology. Proceedings of the British Soc. for Parasitology 81, 525.
Claim 99
Dependent Claim
26 words
Shirley, M.W., Bellatti, M.A. and Millard, B.J. (1982). An egg-shaped (attenuated) line of Eimeria necatrix: further studies on its reproduction pathogenicity and immunogenicity. Parasitology 84, 215.
Claim 100
Dependent Claim
26 words
Shirley, M.W., Bellatti, M.A. and Millard, B.J. (1982). An egg-shaped (attenuated) line of Eimeria necatrix: further studies on its reproduction pathogenicity and immunogenicity. Parasitology 84, 215.
Claim 101
Dependent Claim
22 words
Speer, D.A., Wong, R.B. and Schenkel, R.H. (1983). Effects of monoclonal IgG antibodies on Eimeria tenella (coccidia) sporozoites. J. Parasitol. 69, 775.
Claim 102
Dependent Claim
22 words
Speer, D.A., Wong, R.B. and Schenkel, R.H. (1983). Effects of monoclonal IgG antibodies on Eimeria tenella (coccidia) sporozoites. J. Parasitol. 69, 775.
Claim 103
Dependent Claim
28 words
Speer, C.A., Wong, R.B. and Schenkel, R.H. (1983). Ultrastructural localization of monoclonal IgG antibodies for antigenic sites of Eimeria tenella oocysts, sporocysts and sporozoites. J. Protozoal. 30, 548.
Claim 104
Dependent Claim
28 words
Speer, C.A., Wong, R.B. and Schenkel, R.H. (1983). Ultrastructural localization of monoclonal IgG antibodies for antigenic sites of Eimeria tenella oocysts, sporocysts and sporozoites. J. Protozoal. 30, 548.
Claim 105
Dependent Claim
25 words
Steiner, D.F., Quinn, P.S., Chan, S.J., Marsh, J. and Tager, H.S. (1980). Processing mechanisms in the biosynthesis of proteins. Annals N.Y. Acad. Sci. 343, 1-16.
Claim 106
Dependent Claim
25 words
Steiner, D.F., Quinn, P.S., Chan, S.J., Marsh, J. and Tager, H.S. (1980). Processing mechanisms in the biosynthesis of proteins. Annals N.Y. Acad. Sci. 343, 1-16.
Claim 107
Dependent Claim
27 words
Svennerholm, A., Lange, S. and Holmgrin, J. (1978). Correlation between intestinal synthesis of specific immunoglobulin A and protection against experimental cholera in mice. Inf. Imm. 21, 1.
Claim 108
Dependent Claim
27 words
Svennerholm, A., Lange, S. and Holmgrin, J. (1978). Correlation between intestinal synthesis of specific immunoglobulin A and protection against experimental cholera in mice. Inf. Imm. 21, 1.
Claim 109
Dependent Claim
25 words
Van Deusen, R.A. and Whetstone, C.A. (1981). Practical aspects of producing anti-viral monoclonal antibodies as diagnostic reagents. Proc. Amer. Assn. Vet. Lab. Diagnost. 24, 211.
Claim 110
Dependent Claim
25 words
Van Deusen, R.A. and Whetstone, C.A. (1981). Practical aspects of producing anti-viral monoclonal antibodies as diagnostic reagents. Proc. Amer. Assn. Vet. Lab. Diagnost. 24, 211.
Claim 111
Dependent Claim
24 words
Viera, J. and Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19 259-268.
Claim 112
Dependent Claim
24 words
Viera, J. and Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19 259-268.
Claim 113
Dependent Claim
10 words
Wisher, M.H. (1983). J. Cellular Biochem., Supp. 7A, Abstract 0059.
Claim 114
Dependent Claim
10 words
Wisher, M.H. (1983). J. Cellular Biochem., Supp. 7A, Abstract 0059.
Claim 115
Dependent Claim
11 words
Wong, R.B. and Schenkel, R.H. (1984). Fed. Proc. 184, 43(6), 1630.
Claim 116
Dependent Claim
11 words
Wong, R.B. and Schenkel, R.H. (1984). Fed. Proc. 184, 43(6), 1630.
Claim 117
Dependent Claim
32 words
Wright, I.G., White, M., Tracey-Patte, P.D., Donaldson, R.A., Goodger, B.V., Waltisbuhl, O.J. and Mahoney, D.F. (1983). Babesia bovis: Isolation of a protective antigen by using monoclonal antibody. Infection and Immunity 41, 244.
Claim 118
Dependent Claim
32 words
Wright, I.G., White, M., Tracey-Patte, P.D., Donaldson, R.A., Goodger, B.V., Waltisbuhl, O.J. and Mahoney, D.F. (1983). Babesia bovis: Isolation of a protective antigen by using monoclonal antibody. Infection and Immunity 41, 244.
Claim 119
Dependent Claim
18 words
Wrigley, C.W. (1971). Gel Electrofocusing in: Methods in Enzymology Vol. XXII, W.B. Jacoby ed., Academic Press. pp. 559-564.
Claim 120
Dependent Claim
18 words
Wrigley, C.W. (1971). Gel Electrofocusing in: Methods in Enzymology Vol. XXII, W.B. Jacoby ed., Academic Press. pp. 559-564.
Claim 121
Dependent Claim
4 words
WHAT IS CLAIMED IS:
Claim 122
Dependent Claim
100 words
A purified antigenic protein capable of inducing in a chicken an immune response conferring protection against infection by Eimeria tenella or Eimeria necatrix, the protein having a molecular weight of about 25,000 and being composed of two polypeptides joined by a disulfide bond, one of the polypeptides being characterized by a molecular weight of about 17,000 and by a blocked N-terminal amino acid and having the amino acid sequence set forth in Figure (3) and the other of the polypeptides being characterized by a molecular weight of about 8,000 and having the amino acid sequence set forth in Figure (3).
Claim 123
Dependent Claim
100 words
A purified antigenic protein capable of inducing in a chicken an immune response conferring protection against infection by Eimeria tenella or Eimeria necatrix, the protein having a molecular weight of about 25,000 and being composed of two polypeptides joined by a disulfide bond, one of the polypeptides being characterized by a molecular weight of about 17,000 and by a blocked N-terminal amino acid and having the amino acid sequence set forth in Figure (3) and the other of the polypeptides being characterized by a molecular weight of about 8,000 and having the amino acid sequence set forth in Figure (3).
Claim 124
Dependent Claim
40 words
An antigenic polypeptide having an amino acid sequence included within the amino acid sequence of the protein of claim 1 and capable of inducing in a chicken an immune response conferring protection against infection by Eimeria tenella or Eimeria necatrix.
Claim 125
Dependent Claim
29 words
An antigenic polypeptide having an amino acid sequence included within the amino acid sequence of the polypeptide of claim 1 and characterized by a molecular weight of about 11,500.
Claim 126
Dependent Claim
29 words
An antigenic polypeptide' having an amino acid sequence included within the amino acid sequence of the polypeptide of claim 1 and characterized by a molecular weight of about 6,5CO.
Claim 127
Dependent Claim
29 words
An antigen capable of inducing in a chicken an immune response conferring protection against infection by Eimeria tenella or Eimeria necatrix which comprises an antigenic polypeptide of claim 2.
Claim 128
Dependent Claim
24 words
An antigen of claim 5 which further comprises an amino acid sequence not included within the amino acid sequence set forth in Figure (3).
Claim 129
Dependent Claim
54 words
A method of preparing the protein of claim 1 which comprises: a. contacting sporocysts of Eimeria tenella with a detergent under suitable non-reducing conditions in the presence of protease inhibitors so as to solubilize the sporocyst membrane proteins; and b. separately recovering the protein from the solubilized, sporocyst membrane proteins under suitable non-reducing conditions.
Claim 130
Dependent Claim
23 words
A method of claim 7, wherein the separately recovering comprises partially purifying the solubilized sporocyst membrane proteins by ion exchange and hydroxyapatite chromatography.
Claim 131
Dependent Claim
20 words
A method of claim 7, wherein the separately recovering comprises immunoprecipitation or immunoaffinity chromatography with monoclonal antibody Ptn 7.2 A4/4.
Claim 132
Dependent Claim
55 words
A method of preparing the 17,000 dalton polypeptide of claim 1 which comprises: a. contacting sporocysts of Eimeria tenella with a detergent under suitable conditions in the presence of protease inhibitors so as to solubilize the sporocyst membrane proteins; and b. separately recovering the polypeptide from the solubilized, sporocyst membrane proteins under suitable reducing conditions.
Claim 133
Dependent Claim
29 words
A method of claim 10, wherein the separately recovering comprises partially purifying the solubilized sporocyst membrane proteins by chromatography on DEAE-cellulose followed by preparative SDS-electrophoresis under suitable reducing conditions.
Claim 134
Dependent Claim
57 words
A method of preparing the polypeptide of claim 3 which comprises: a. contacting sporocysts of Eimeria tenella with phenol in the presence of 8-hydroxyquinoline, so as to extract the sporozoite membrane proteins from the sporocysts; and b. recovering the polypeptide from the extracted sporocyst membrane proteins by immunoprecipitation or immunoaffinity chromatography with monoclonal antibody Ptn 7.2 A4/4.
Claim 135
Dependent Claim
49 words
A method of preparing the polypeptide of claim 4 which comprises: a. contacting trypsin-taurodeoxycholate excysted, sporozoite membrane proteins with detergent so as to solubilize the proteins; and b. recovering the polypeptide from the solubilized, excysted sporozoite membrane proteins by immunoprecipitation or immunoaffinity chromatography with monoclonal antibody Ptn 7.2 A4/4.
Claim 136
Dependent Claim
56 words
A method of preparing the protein of claim 1 which comprises preparing a DNA molecule coding for the protein, inserting the DNA molecule into an appropriate expression vector, introducing the resulting expression vector into a suitable host under appropriate conditions permitting expression of the DNA and production of the protein and recovering the protein so produced.
Claim 137
Dependent Claim
56 words
A method of preparing the polypeptide of claim 2 which comprises preparing a DNA molecule coding for the polypeptide, inserting the DNA molecule into an appropriate expression vector, introducing the resulting expression vector into a suitable host under appropriate conditions permitting expression of the DNA and production of the polypeptide and recovering the polypeptide so produced.
Claim 138
Dependent Claim
58 words
A method of preparing the 17,000 dalton polypeptide of claim 1 which comprises preparing a DNA molecule coding for the polypeptide, inserting the DNA molecule into an appropriate expression vector, introducing the resulting expression vector into a suitable host under appropriate conditions permitting expression of the DNA and production of the polypeptide and recovering the polypeptide so produced.
Claim 139
Dependent Claim
30 words
A method of conferring upon a chicken active immunity against infection by Eimeria tenella which comprises administering to a chicken an effective immunizing amount of the protein of claim 1.
Claim 140
Dependent Claim
30 words
A method of conferring upon a chicken active immunity against infection by Eimeria necatrix which comprises administering to a chicken an effective immunizing amount of the protein of claim 1.
Claim 141
Dependent Claim
33 words
A method of conferring upon a chicken active immunity against infection by Eimeria tenella or Eimeria necatrix which comprises administering to a chicken an effective immunizing amount of the polypeptide of claim 2.
Claim 142
Dependent Claim
33 words
A method of conferring upon a chicken active immunity against infection by Eimeria tenella or Eimeria necatrix which comprises administering to a chicken an effective immunizing amount of the polypeptide of claim 5.
Claim 143
Dependent Claim
21 words
A method of claim 17, wherein the effective immunizing amount is an amount from about 0.1 µg to about 1.0 mg.
Claim 144
Dependent Claim
21 words
A method of claim 18, wherein the effective immunizing amount is an amount from about 0.1 pg to about 1.0 mg.
Claim 145
Dependent Claim
31 words
A vaccine for conferring upon a chicken active immunity against infection by Eimeria tenella which comprises per dose an effective immunizing amount of the protein of claim 1 and suitable carrier.
Claim 146
Dependent Claim
32 words
A vaccine for conferring upon a chicken active immunity against infection by Eimeria necatrix which comprises per dose an effective immunizing amount of the protein of claim 1 and a suitable carrier.
Claim 147
Dependent Claim
33 words
A vaccine for conferring upon a chicken active immunity against infection by Eimeria tenella or Eimeria necatrix which comprises an effective immunizing amount of the polypeptide of claim 2 and a suitable carrier.
Claim 148
Dependent Claim
33 words
A vaccine for conferring upon a chicken active immunity against infection by Eimeria tenella or Eimeria necatrix which comprises an effective immunizing amount of the polypeptide of claim 5 and a suitable carrier.
Claim 149
Dependent Claim
21 words
A vaccine of claim 23, wherein the effective immunizing amount is above about 0.1 pg/kg of body weight of the chicken.
Claim 150
Dependent Claim
21 words
A vaccine of claim 24, wherein the effective immunizing amount is above about 0.1 µg/kg of body weight of the chicken.
Claim 151
Dependent Claim
26 words
A method of protecting a chicken against infection by Eimeria tenella which comprises administering to the chicken a suitable dose of the vaccine of claim 23.
Claim 152
Dependent Claim
26 words
A method of protecting a chicken against infection by Eimeria necatrix which comprises administering to the chicken a suitable dose of the vaccine of claim 24.
Claim 153
Dependent Claim
10 words
A monoclonal antibody directed against the protein of claim 1.
Claim 154
Dependent Claim
10 words
A monoclonal antibody directed against the polypeptide of claim 2.
Claim 155
Dependent Claim
13 words
The monoclonal antibody Ptn 7.2A4/4 produced by hybridoma cell line ATCC No. HB8561.
Claim 156
Dependent Claim
13 words
The monoclonal antibody Ptn 7.2A4/4 produced by hybridoma cell line ATCC No. HB8561.
Claim 157
Dependent Claim
36 words
A method of conferring upon a chicken passive immunity against infection by Eimeria tenella or Eimeria necatrix wnich comprises administering to the chicken an effective protecting amount of the antibody of claim 31, 32 or 33.
Claim 158
Dependent Claim
37 words
A composition for conferring upon a chicken passive immunity against infection by Eimeria tenella or Eimeria necatrix which comprises an effective protecting amount of the monoclonal antibody of claim 31, 32 or 33 and a suitable carrier.
Claim 159
Dependent Claim
29 words
A method of conferring passive immunity against infection by Eimeria tenella or Eimeria necatrix which comprises administering to a chicken a suitable dose of the composition of claim 35.
Claim 160
Dependent Claim
11 words
An anti-idiotypic antibody directed against the monoclonal antibody of claim 33.
Claim 161
Dependent Claim
64 words
A method of preparing the anti-idiotypic antibody of claim 37 which comprises: a. recovering the monoclonal antibody Ptn 7.2A4/4 from hybridoma cell line ATCC No. HB8561; b. purifying the monoclonal antibody; c. injecting the purified monoclonal antibody into a suitable animal together with a suitable adjuvant; d. removing blood serum from the injected animal; and e. recovering the anti-idiotypic antibody from the blood serum.
Claim 162
Dependent Claim
34 words
A method of conferring upon a chicken active immunity against infection by Eimeria tenella or Eimeria necatrix which comprises administering to a chicken an effective immunizing amount of the anti-idiotypic antibody of claim 37.
Claim 163
Dependent Claim
34 words
A vaccine for conferring upon a chicken active immunity against infection by Eimeria tenella or Eimeria necatrix which comprises an effective immunizing amount of the anti-idiotypic antibody of claim 37 and a suitable carrier.
Claim 164
Dependent Claim
29 words
A method of protecting a chicken against infection by Eimeria tenella or Eimeria necatrix which comprises administering to a chicken a suitable dose of the vaccine of claim 40.
Claim 165
Dependent Claim
10 words
A nucleic acid molecule encoding the protein of claim 1.
Claim 166
Dependent Claim
16 words
A DNA molecule of claim 42 having the nucleic acid sequence set forth in Figure (3).
Claim 167
Dependent Claim
6 words
A cDNA molecule of claim 42.
Claim 168
Dependent Claim
16 words
A DNA molecule encoding the polypeptide of any of claims 2, 3, 4, 5 or 6.
Claim 169
Dependent Claim
11 words
A cloning vehicle which comprises the nucleic acid of claim 42.
Claim 170
Dependent Claim
11 words
A host cell which comprises the cloning vehicle of claim 46.
Claim 171
Dependent Claim
6 words
A bacterial host of claim 47.
Claim 172
Dependent Claim
39 words
A method of producing the protein having the amino acid sequence set forth in Figure (3) which comprises growing host cells of claim 47 under suitable conditions permitting production of the protein and recovering the protein so produced. .
Claim 173
Dependent Claim
19 words
A protein of claim 1 having esentially the same sequence but differing because of expression in a bacterial host.
Claim 174
Dependent Claim
94 words
A method for obtaining the DNA of claim 43 which comprises isolating total genomic DNA from Eimeria tenella oocysts; preparing DNA fragments from the genomic DNA so isolated; ligating the fragments so prepared into an appropriate cloning vector; subjecting the DNA of the clones so prepared to hybridization with oligonucleotides containing, or complementary to, nucleic acid sequences present within the nucleic acid sequence set forth in Figure (3) to identify appropriate clones; and isolating from the appropriate clones DNA which encodes the protein and has the nucleic acid sequence set forth in Figure (3).
References Cited (Backward Citations)
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