Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
86 words
A test device for determining the presence of ethanol in an aqueous test sample, the test device comprising a carrier matrix incorporated with alcohol oxidase, a peroxidatively active substance and a chromogenic indicator system capable of providing a detectable response wherein a chromogenic component of the indicator system is substantially in the reduced form, the alcohol oxidase being present in a quantity sufficient to provide a detectable colorimetric response to the presence of 100 mg/dL ethanol in the test sample in less than about 5 minutes.
Claim 2
Dependent Claim
86 words
A test device for determining the presence of ethanol in an aqueous test sample, the test device comprising a carrier matrix incorporated with alcohol oxidase, a peroxidatively active substance and a chromogenic indicator system capable of providing a detectable response wherein a chromogenic component of the indicator system is substantially in the reduced form, the alcohol oxidase being present in a quantity sufficient to provide a detectable colorimetric response to the presence of 100 mg/dL ethanol in the test sample in less than about 5 minutes.
Claim 3
Dependent Claim
36 words
The test device of claim 1 in which the amount of alcohol oxidase present is that amount resulting from the incorporation of the carrier with a solution containing at least 10 IU/mL alcohol oxidase and drying.
Claim 4
Dependent Claim
19 words
The test device of claims 1 or 2 in which the carrier matrix is additionally incorporated with an azide.
Claim 5
Dependent Claim
34 words
The test device of claims 1 to 3 in which the carrier matrix is additionally incorporated with a buffering substance capable of providing a pH in the range of from about 5 to 9.
Claim 6
Dependent Claim
19 words
The test device of claims 1 to 4 which the carrier matrix is additionally incorporated with a stabilizing agent.
Claim 7
Dependent Claim
86 words
A method for preparing the test device of claim 1 which comprises the steps of: (a) preparing a first mixture of a component of a chromogenic indicator system and an organic solvent; (b) preparing an aqueous second mixture of a peroxidatively active substance, and any remaining components of the chromogenic indicator system, and alcohol oxidase; (c) incorporating the carrier matrix with one of the first or second mixtures and drying; and (d) incorporating the carrier with the other of the first or second mixtures and drying.
Claim 8
Dependent Claim
18 words
The method of claim 6 in which the aqueous second mixture contains at least 10 IU/mL alcohol oxidase.
Claim 9
Independent Claim
19 words
The method of claims 6 or 7 in which an azide is additionally included in the aqueous second mixture.
Claim 10
Independent Claim
39 words
The method of claims 6 to 8 in which a buffering substance capable of providing a pH in the range of from about 5 to 9 and optionally a stabilizing agent are additionally included in the aqueous second mixture.
Claim 11
Dependent Claim
75 words
The use of a test device comprising a carrier matrix incorporated with alcohol oxidase, a peroxidatively active substance and a chromogenic indicator system capable of providing a detectable response wherein a chromogenic component of the indicator system is substantially in the reduced .form, the alcohol oxidase being present in a quantity sufficient to provide a detectable colorimetric response to the presence of 100 mg/dL ethanol in the test sample in less than about 5 minutes.
Claim 12
Dependent Claim
75 words
The use of a test device comprising a carrier matrix incorporated with alcohol oxidase, a peroxidatively active substance and a chromogenic indicator system capable of providing a detectable response wherein a chromogenic component of the indicator system is substantially in the reduced .form, the alcohol oxidase being present in a quantity sufficient to provide a detectable colorimetric response to the presence of 100 mg/dL ethanol in the test sample in less than about 5 minutes.