EP0163573A1
Expired / Lapsed
EP
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Site-specific proteolysis by renin
Assignee: Merck and Co Inc
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Filing: 1985-05-20
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Granted: —
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Expires: 2005-05-20
Abstract
Abstract A nucleotide sequence which codes for an amino acid sequence specifically recognized and cleaved by the endopeptidase, renin, has been inserted into a plasmid expression vector. This nucleotide sequence was placed within a sequence of DNA, at the junction between the sequences coding for two distinct domains of a fusion protein. The resultant novel protein product expressed by the new construction can be specifically cleaved by renin to yield two distinct proteins corresponding to the two domains of the original fusion protein product.
Inventors
- 👤 Joshua S. Boger
- 👤 Mary L. Haffey
- 👤 Edward M. Scolnick
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 11:03
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C07; C07K; C07K14/00; C07K14/005; C07K7/00; C07K7/04; C07K7/14; C07K2319/00; C07K2319/50; C07K2319/61; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16211; C12N2710/16222
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
15 words
A DNA sequence which codes for an amino acid sequence cleavable by the endopeptidase, renin.
Claim 2
Dependent Claim
15 words
A DNA sequence which codes for an amino acid sequence cleavable by the endopeptidase, renin.
Claim 3
Dependent Claim
23 words
A DNA oligonucleotide according to Claim 1 comprising a nucleotide sequence selected from: an equivalent nucleotide sequence encoding the same amino acid sequence.
Claim 4
Dependent Claim
11 words
An oligonucleotide coding for a peptide comprising the amino acid sequence:
Claim 5
Dependent Claim
11 words
An oligonucleotide coding for a peptide comprising the amino acid sequence:
Claim 6
Dependent Claim
30 words
A vector containing a plurality of genes coding for a protein comprised of a plurality of distinct domains, the vector having an oligonucleotide of Claim 2 inserted between each domain.
Claim 7
Dependent Claim
18 words
A vector according to Claim 4 continging two genes coding for a protein comprised of two distinct domains.
Claim 8
Dependent Claim
19 words
A vector according to Claim 4 wherein the oligonucleotide is inserted at the precise junction of the two domains.
Claim 9
Dependent Claim
11 words
A procaryotic or eucaryotic host containing the vector of Claim 4.
References Cited (Backward Citations)
EP0089626A2
Cited
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