EP0163543A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Marrow cell stimulator
Assignee: Vxr Inc
•
Filing: 1985-05-31
•
Granted: —
•
Expires: 2005-05-31
Abstract
Abstract A biologically active composition isolated from thymus tissue, capable of enhancing the growth of pluripotent and lymphopoietic cells in bone marrow culture systems, comprising a factor which is heat stable to approximately 80°C, which has a high charge-to-mass ratio, and which is essentially free of serum albumin. A process for isolating the composition from thymus tissue is described together with compositions comprising the biologically active composition for use as a supplement for in vitro cultures, such as bone marrow cultures and as a therapeutic composition from bone marrow dysfunction and for use in bone marrow transplant procedures. An antibody to the biologically active composition is described together with therapeutic and diagnostic reagents comprising same.
Inventors
- 👤 Marcia F. Goldfarb
- 👤 Richard J. Decarolis
- 👤 Harold C. Miller
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 22:37
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K4/00; C07K4/12
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
47 words
A biologically active composition isolated from thymus tissue, capable of enhancing the growth of pluripotent and lymphopoietic cells in bone marrow culture systems, comprising a factor which is heat stable to approximately 80°C, which has a high charge-to-mass ratio, and which is essentially free of serum albumin.
Claim 2
Dependent Claim
47 words
A biologically active composition isolated from thymus tissue, capable of enhancing the growth of pluripotent and lymphopoietic cells in bone marrow culture systems, comprising a factor which is heat stable to approximately 80°C, which has a high charge-to-mass ratio, and which is essentially free of serum albumin.
Claim 3
Dependent Claim
11 words
A composition as in claim 1, isolated from calf thymus tissue.
Claim 4
Dependent Claim
55 words
A process for isolating a marrow.-cell stimulator composition from thymus tissue, the steps of which comprise: A. preparing a crude thymic extract; B. subjecting said crude thymic extract to preparative electrophoresis; C. collecting the material which migrates faster in the electric field than does bovine or animal serum albumin, and D. concentrating said collected material.
Claim 5
Dependent Claim
55 words
A process for isolating a marrow.-cell stimulator composition from thymus tissue, the steps of which comprise: A. preparing a crude thymic extract; B. subjecting said crude thymic extract to preparative electrophoresis; C. collecting the material which migrates faster in the electric field than does bovine or animal serum albumin, and D. concentrating said collected material.
Claim 6
Dependent Claim
18 words
A process as in claim 3, wherein the marrow cell stimulator factor is isolated from calf thymus tissue.
Claim 7
Dependent Claim
136 words
A process as in claim 3, wherein the crude thymic extract of step A is prepared as follows: A. removing connective and other extraneous tissues from thymus glands of freshly slaughtered neonatal vertebrates; B. homogenizing said thymus glands in a buffered solution; C. centrifuging the homogenate to remove cellular debris; D. heating the supernatant from step C to approximately 80°C until soluble proteins, including proteases, have congealed; E . removing said congealed material by centrifugation; F. freezing the supernatant from step E; G. thawing the supernatant and removing any solids by high speed centrifugation; H. concentrating the supernatant from step G by ultrafiltration using a membrane that retains molecules with molecular weights larger than 10,000 daltons, and I. dialyzing the concentrate from step H to eliminate molecules of molecular weights less than 12,000 to 14,000 daltons.
Claim 8
Dependent Claim
16 words
A process as in claim 3, wherein a powder-block electrophoresis apparatus 's employed in step B.
Claim 9
Dependent Claim
12 words
A marrow cell stimulator composition isolated by the process of claim 3.
Claim 10
Dependent Claim
18 words
A supplement for in vitro cell cultures, comprising a composition as in either of claims 1 or 7.
Claim 11
Dependent Claim
11 words
A supplement as in claim 8, for bone marrow cell cultures.
Claim 12
Dependent Claim
17 words
A supplement as in claim 9, to maintain and support proliferation of a pluripotent stem cell population.
Claim 13
Dependent Claim
11 words
A supplement as in claim 9, to maintain and support lymphopoiesis.
Claim 14
Dependent Claim
14 words
A therapeutic composition comprising a composition as in either of claims 1 or 7.
Claim 15
Dependent Claim
12 words
A therapeutic composition as in claim 12, for bone marrow transplant procedures.
Claim 16
Dependent Claim
18 words
A therapeutic composition as in claim 12, for in vivo treatment of immunodeficiencies resulting from bone marrow dysfunction.
Claim 17
Dependent Claim
16 words
A therapeutic composition comprising an antibody to the composition of either of claims 1 or 7.
Claim 18
Dependent Claim
17 words
A clinical diagnostic composition comprising an antibody to the composition of either of claims 1 or 7.
Claim 19
Dependent Claim
15 words
A composition as in either of claims 1 or 7 for use as a pharmaceutical.
Claim 20
Dependent Claim
37 words
A process for preparing a therapeutic composition according to any of claims 12 to 14 comprising the step of bringing a composition as in either of claims 1 or 7 into association with a pharmaceutically acceptable excipient.
Claim 21
Dependent Claim
36 words
A process for preparing a therapeutic composition according to claim 15 comprising the step of bringing an antibody to a composition as in either of claims 1 or 7 into association with a pharmaceutically acceptable excipient.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.