EP0159418A1
Expired / Lapsed
EP
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A process for the incorporation of foreign DNA into the genome of monocotyledonous plants
Assignee: Leiden University
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Filing: 1984-06-04
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Granted: —
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Expires: 2004-06-04
Abstract
Abstract A process for the incorporation of foreign DNA into the genome of monocotyledonous plants with bacteria, which are provided with one or more tumour-inducing plasmides or derivatives thereof, initially originating from Agrobacterium, or of bacteria which have the T-DNA, originating from the above-meant plasmides, and/or the virulence genes, originating from the above-mentioned plasmides incorporated into the bacterial DNA elsewhere.
Inventors
- 👤 Paul Jan Jacob Dr. Hooykaas
- 👤 Geertrui Martha Sibrig Dr. Van Slogteren
- 👤 Robbert Adriaan Prof. Dr. Schilperoort
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 04:19
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8201; C12N15/8202; C12N15/8205
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
97 words
A process for the incorporation of foreign DNA into the genome of plants by infecting these plants or incubating the plant protoplasts with bacteria suitable or made suitable for that purpose, characterized in that monocotyledonous plants are infected or monocotyledonous plant protoplasts are incubated with bacteria suitable or made suitable for that purpose, which are provided with one or more tumour-inducing plasmids or derivatives thereof, originally originating from Agrobacterium, or from bacteria, which have the T-DNA, originating from the above-meant plasmids and/or the virulence genes originating from the above mentioned plasmids incorporated elsewhere in the bacterial DNA.
Claim 2
Dependent Claim
97 words
A process for the incorporation of foreign DNA into the genome of plants by infecting these plants or incubating the plant protoplasts with bacteria suitable or made suitable for that purpose, characterized in that monocotyledonous plants are infected or monocotyledonous plant protoplasts are incubated with bacteria suitable or made suitable for that purpose, which are provided with one or more tumour-inducing plasmids or derivatives thereof, originally originating from Agrobacterium, or from bacteria, which have the T-DNA, originating from the above-meant plasmids and/or the virulence genes originating from the above mentioned plasmids incorporated elsewhere in the bacterial DNA.
Claim 3
Dependent Claim
22 words
A process according to claim 1, characterized in that for the infection or incubation use is made of Agrobacterium or Rhizobium bacteria.
Claim 4
Dependent Claim
27 words
A process according to claim 1 or 2, characterized in that bacteria are used which are provided with one or more Ti- or Ri-plasmids or derivatives thereof.
Claim 5
Dependent Claim
38 words
A process according to claim 3, characterized in that the bacteria used are provided with a stable cointegrate plasmid constructed from a plasmid R772 and a plasmid pTiB6 with foreign DNA incorporated into the T-region of the latter.
Claim 6
Dependent Claim
53 words
A process according to any of the preceding claims, characterized in that bacteria are used, which contain at least one plasmid, which has the Vir-region of a Ti (tumour inducing) plasmid but no T-region, and at least one other plasmid, which has a T-region with built in therein foreign DNA but no Vir-region.
Claim 7
Dependent Claim
32 words
Monocotyledonous plant and plant cells obtained after that by applying the process according to any of the preceding claims the genetic properties of the original plants c.q. plant cells have been modified.
Claim 8
Dependent Claim
35 words
A process for the preparation of chemical and/or pharmaceutical products, characterized in that cells obtained by application of the process according to any of the claims 1-5 are cultivated and the desirable substance is isolated.
Claim 9
Dependent Claim
22 words
A process according to claim 7, characterized in that the cultivating is carried out by means of fermentation and optionally subsequent immobilisation.
Claim 10
Dependent Claim
49 words
A process according to any of the claims 1-5 incl., 7 or 8, characterized in that the regulator regions positioned before and after the protein encoding regions of T-DNA-genes in particular the genes for octopine synthesis and nopaline synthesis are used for expressing foreign genes in monocotyledonous plant cells.
References Cited (Backward Citations)
No citation records indexed.
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