EP0158892A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
59 Valine insulin-like growth factor I and process for production thereof
Assignee: Astellas Pharma Inc
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Filing: 1985-03-28
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Granted: —
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Expires: 2005-03-28
Abstract
Abstract 59 Valine insulin-like growth factor I (thereinafter referred to 59 Val-IGF-I), processes for the production thereof, a gene which codes for the expression of 59 Val-IGF-I, a plasmid containing 59 Val-IGF-I gene, an organism containing therein a plasmid which includes 59 Val-IGF-I gene, and prosesses for the production thereof.
Inventors
- 👤 Ikuo Ueda
- 👤 Mineo Niwa
- 👤 Yoshimasa Saito
- 👤 Susumu Sato
- 👤 Hiroki Ono
- 👤 Tadashi Kitaguchi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 13:35
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/65; C07K14/59; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C07K2319/00; C07K2319/01; C07K2319/036; C07K2319/70; C07K2319/74; C07K2319/75; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/12; Y10S930/26
Claims Analysis
Total Claims: 46
Claim 1
Independent Claim
22 words
59 Valine insulin-like growth factor I (hereinafter referred to 59 Val-IGF-I), wherein 59 Val-IGF-I consists 70 amino acids in the following sequence:
Claim 2
Dependent Claim
22 words
59 Valine insulin-like growth factor I (hereinafter referred to 59 Val-IGF-I), wherein 59 Val-IGF-I consists 70 amino acids in the following sequence:
Claim 3
Dependent Claim
107 words
A process for the production of 59 Val-insulin-like growth factor I (hereinafter referred to 59 Val-IGF-I) characterized in that it comprises (a) culturing an organism containing therein a plasmid which includes a synthetic 59 Val-IGF-I gene and a promoter gene upstream of and adjacent to said 59 Val-IGF-I gene to control expression of 59 V al- IGF - I gene, in which said synthetic 59 Val-IGF-I gene codes for the expression of 59 Val-IGF-I, wherein 59 Val-IGF-I consists 70 amino acids in the following sequence: and said promoter gene has no structural gene, and (b) recovering 59 Val-TGF-I as a non fused peptide from the culture broth.
Claim 4
Dependent Claim
107 words
A process for the production of 59 Val-insulin-like growth factor I (hereinafter referred to 59 Val-IGF-I) characterized in that it comprises (a) culturing an organism containing therein a plasmid which includes a synthetic 59 Val-IGF-I gene and a promoter gene upstream of and adjacent to said 59 Val-IGF-I gene to control expression of 59 V al- IGF - I gene, in which said synthetic 59 Val-IGF-I gene codes for the expression of 59 Val-IGF-I, wherein 59 Val-IGF-I consists 70 amino acids in the following sequence: and said promoter gene has no structural gene, and (b) recovering 59 Val-TGF-I as a non fused peptide from the culture broth.
Claim 5
Dependent Claim
37 words
A process as claimed in Claim 2 in which (a) the host organism is Escherichia. coli, (b) the 59 Val-IGF-I gene comprises the following sequence: and (c) the promoter gene comprises the following sequence: * EcoRI EcoRI
Claim 6
Dependent Claim
27 words
A synthetic gene which codes for the expression of 59 Val-IGF-I, wherein 59 Val-IGF-I consists of 70 amino acids in the following sequence: or a subunit thereof.
Claim 7
Dependent Claim
27 words
A synthetic gene which codes for the expression of 59 Val-IGF-I, wherein 59 Val-IGF-I consists of 70 amino acids in the following sequence: or a subunit thereof.
Claim 8
Dependent Claim
18 words
A synthetic gene of claim 4, in which the gene comprises the following sequence: or a subunit thereof.
Claim 9
Dependent Claim
20 words
A synthetic gene of Claims 4 to 5, in which the gene comprises the following sequence: or a sub-unit thereof.
Claim 10
Dependent Claim
33 words
A process for the production of a synthetic gene as claimed in any of Claims 4 to 6 characterized in that it comprises the hybridization and ligation of a number of oligonucleotide blocks.
Claim 11
Dependent Claim
31 words
A plasmid containing 59 Val-IGF-I gene, in which said synthetic 59 Val-IGF-I gene codes for the expression of 59 Val-IGF-I, wherein 59 Val-IGF-I consists 70 amino acids in the following sequence:
Claim 12
Dependent Claim
31 words
A plasmid containing 59 Val-IGF-I gene, in which said synthetic 59 Val-IGF-I gene codes for the expression of 59 Val-IGF-I, wherein 59 Val-IGF-I consists 70 amino acids in the following sequence:
Claim 13
Dependent Claim
41 words
An organism which characterized in that it containing therein a plasmid which includes 59 Val-IGF-I gene, in which said synthetic 59 Val-IGF-I gene codes for the expression of 59 Val-IGF-I, wherein 59 Val-IGF-I consists 70 amino acids in the following sequence:
Claim 14
Dependent Claim
41 words
An organism which characterized in that it containing therein a plasmid which includes 59 Val-IGF-I gene, in which said synthetic 59 Val-IGF-I gene codes for the expression of 59 Val-IGF-I, wherein 59 Val-IGF-I consists 70 amino acids in the following sequence:
Claim 15
Dependent Claim
74 words
10 . 59 Val-IGF-I expression plasmid which characterized in that it includes a synthetic 59 Val-IGF-I gene and a promoter gene upstream of and adjacent to said 59 Val-IGF-I gene to control expression of 59 Val - IGF - I gene, in which said synthetic 59 Val-IGF-I gene codes for the expression of 59 Val-IGF-I, wherein 59 Val-IGF-I consists 70 amino acids in the following sequence: and said promoter gene has no structural gene.
Claim 16
Dependent Claim
74 words
10 . 59 Val-IGF-I expression plasmid which characterized in that it includes a synthetic 59 Val-IGF-I gene and a promoter gene upstream of and adjacent to said 59 Val-IGF-I gene to control expression of 59 Val - IGF - I gene, in which said synthetic 59 Val-IGF-I gene codes for the expression of 59 Val-IGF-I, wherein 59 Val-IGF-I consists 70 amino acids in the following sequence: and said promoter gene has no structural gene.
Claim 17
Dependent Claim
72 words
An organism which characterized in that it containing therein a plasmid which includes 59 Val-IGF-I gene and a promoter gene upstream of and adjacent to said 59 Val-IGF-I gene to control expression of 59 Val- IGF - I gene, in which said synthetic 59 Val-IGF-I gene codes for the expression of 59 Val-IGF-I, wherein 59 Val-TGF-I consists 70 amino acids in the following sequence: and said promoter gene has no structural gene.
Claim 18
Dependent Claim
72 words
An organism which characterized in that it containing therein a plasmid which includes 59 Val-IGF-I gene and a promoter gene upstream of and adjacent to said 59 Val-IGF-I gene to control expression of 59 Val- IGF - I gene, in which said synthetic 59 Val-IGF-I gene codes for the expression of 59 Val-IGF-I, wherein 59 Val-TGF-I consists 70 amino acids in the following sequence: and said promoter gene has no structural gene.
Claim 19
Dependent Claim
23 words
A synthetic promoter gene to control expression of 59 Val-IGF-I gene, in which the promoter gene comprises the following sequence: * EcoRI EcoRI
Claim 20
Dependent Claim
23 words
A synthetic promoter gene to control expression of 59 Val-IGF-I gene, in which the promoter gene comprises the following sequence: * EcoRI EcoRI
Claim 21
Dependent Claim
29 words
A. process for the production of a synthetic gene as claimed in claim 12 characterized in that it comprises the hybridization and ligation of a number of oligonucleotide blocks.
Claim 22
Dependent Claim
19 words
A plasmid containing a synthetic promoter gene, in which the promoter gene comprises the following sequence: * EcoRI EcoRI
Claim 23
Dependent Claim
19 words
A plasmid containing a synthetic promoter gene, in which the promoter gene comprises the following sequence: * EcoRI EcoRI
Claim 24
Dependent Claim
28 words
An organism characterized in that it contains therein a plasmid which includes a synthetic promoter gene, in which the promoter gene comprises the following sequence: * EcoRI EcoRI
Claim 25
Dependent Claim
28 words
An organism characterized in that it contains therein a plasmid which includes a synthetic promoter gene, in which the promoter gene comprises the following sequence: * EcoRI EcoRI
Claim 26
Dependent Claim
45 words
A process for the production of 59 Val-IGF-I expression plasmid as claimed in Claim 10 characterized in that it comprises the insertion of 59 Val-IGF-I gene as claimed in Claims 4 to 6 and promoter gene as claimed in Claim 12 into a suitable plasmid.
Claim 27
Dependent Claim
33 words
A process for the production of an organism for producing 59 Val-IGF-I characterized in that it comprises transformation of the 59 Val-IGF-I expression plasmid as claimed in Claim 10 into a suitable organism.
Claim 28
Dependent Claim
21 words
A process for the production of 59 Val-IGF-I which comprises subjecting 59 Val-IGF-I fused with a protective peptide to elimination reaction.
Claim 29
Dependent Claim
21 words
A process for the production of 59 Val-IGF-I which comprises subjecting 59 Val-IGF-I fused with a protective peptide to elimination reaction.
Claim 30
Dependent Claim
44 words
A process of claim 18, in which i) the protective peptide is a protein peptide having methionine as the last amino acid and fused with 59 Val-IGF-I through said methionine of the protein peptide, and ii) the elimination reaction is conducted with cyanogen bromide.
Claim 31
Dependent Claim
9 words
20 . 59 Val-IGF-I fused with a protective peptide.
Claim 32
Dependent Claim
9 words
20 . 59 Val-IGF-I fused with a protective peptide.
Claim 33
Dependent Claim
39 words
21 . 59 Val-IGF-I fused with a protective peptide of claim 20, in which the protective peptide is a protein peptide having methionine as the last amino acid and fused with 59 Val-IGF-I through said methionine of the protein-peptide.
Claim 34
Dependent Claim
25 words
22 . 59 Val-IGF-I fused with a protective peptide of claim 21, which is IGF-I fused with protein peptide LH having following amino acid sequence:
Claim 35
Dependent Claim
24 words
23 . 59 Val-IGF-I fused with a protective peptide of claim 21, which is 59 Val-IGF-I fused with B-galactosidase having following amino acid sequence:
Claim 36
Dependent Claim
11 words
A gene coding for 59 Val-IGF-I fused with a protective peptide.
Claim 37
Dependent Claim
11 words
A gene coding for 59 Val-IGF-I fused with a protective peptide.
Claim 38
Dependent Claim
59 words
A process for the production of a gene coding for 59 Val-IGF-I fused with a protective peptide which comprises linking a gene coding for a protective peptide with a gene coding for 59 Val-IGF-I upstream of said 59 Val-IGF-I gene using a linker, in which said 59 I GF- I gene has or has not a terminator downstream thereof.
Claim 39
Dependent Claim
59 words
A process for the production of a gene coding for 59 Val-IGF-I fused with a protective peptide which comprises linking a gene coding for a protective peptide with a gene coding for 59 Val-IGF-I upstream of said 59 Val-IGF-I gene using a linker, in which said 59 I GF- I gene has or has not a terminator downstream thereof.
Claim 40
Dependent Claim
19 words
An expression vector containing a promoter gene and a gene coding for 59 Val-IGF-I fused with a protective peptide.
Claim 41
Dependent Claim
19 words
An expression vector containing a promoter gene and a gene coding for 59 Val-IGF-I fused with a protective peptide.
Claim 42
Dependent Claim
30 words
A process for the production of an expression vector which comprises inserting a promoter gene and a gene coding for 59 Val-IGF-I fused with a protective peptide into a plasmid.
Claim 43
Dependent Claim
30 words
A process for the production of an expression vector which comprises inserting a promoter gene and a gene coding for 59 Val-IGF-I fused with a protective peptide into a plasmid.
Claim 44
Dependent Claim
11 words
A transformant containing an expression vector as defined in Claim 26.
Claim 45
Dependent Claim
23 words
A process for the production of a transformant which comprises transforming a host organism with an expression vector as defined in Claim 28.
Claim 46
Dependent Claim
23 words
A process for the production of 59 Val-IGF-I fused with a protective peptide which comprises culturing a transformant as defined in Claim 28.
References Cited (Backward Citations)
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