EP0157629A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Chemiluminescent methods and kit
Assignee: 3M Co
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Filing: 1985-04-02
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Granted: —
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Expires: 2005-04-02
Abstract
Abstract Methods for the detection and measurement of compounds containing beta-lactam rings by chemiluminescence of the compounds in the presence of a chemiluminescent compound such as luminol, and for improving the sensitivity of assays using chemiluminescent reactions such as the luminol reaction utilizing compounds containing beta-lactam rings are described. A kit for conducting such chemiluminescent assays is also described.
Inventors
- 👤 Dean S. Milbrath
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 22:47
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/02; G01N33/04; G01N33/48; G01N33/50; G01N33/52
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
141 words
A method for the detection of a compound containing a beta-lactam ring in a sample suspected of containing a beta-lactam comprising the steps of a) combining together as a mixture i) a reaction system comprising a chemiluminescent compound, a peroxide source and a base, and ii) a predetermined portion of said sample, and b) measuring the light output of said mixture; said reaction system being further characterized as one which, if said predetermined portion of said sample contained, as the only beta-lactam, penicillin G in an amount-such that said mixture contained penicillin G in a concentration of 0.45 mM, produces a light output signal which, when measured by a luminometer and integrated over 30 seconds beginning with the initiation of the signal, is at least about two times that produced if said predetermined portion of said sample was free of beta-lactam.
Claim 2
Independent Claim
141 words
A method for the detection of a compound containing a beta-lactam ring in a sample suspected of containing a beta-lactam comprising the steps of a) combining together as a mixture i) a reaction system comprising a chemiluminescent compound, a peroxide source and a base, and ii) a predetermined portion of said sample, and b) measuring the light output of said mixture; said reaction system being further characterized as one which, if said predetermined portion of said sample contained, as the only beta-lactam, penicillin G in an amount-such that said mixture contained penicillin G in a concentration of 0.45 mM, produces a light output signal which, when measured by a luminometer and integrated over 30 seconds beginning with the initiation of the signal, is at least about two times that produced if said predetermined portion of said sample was free of beta-lactam.
Claim 3
Dependent Claim
33 words
A method according to Claim 1, wherein the chemiluminescent compound is selected from the group consisting of luminol, a luminol analog, isoluminol, an isoluminol analog, coumarin 2, sulforhodamine 101, umbelliferone, 4-methylumbelliferone and fluorescein.
Claim 4
Dependent Claim
14 words
A method according to Claim 1, wherein said beta-lactam being detected is an antibiotic.
Claim 5
Dependent Claim
19 words
A method according to Claim 1, wherein said beta-lactam being detected is identified by its characteristic light output trace.
Claim 6
Dependent Claim
27 words
A method according to Claim 1, wherein the quantity of said beta-lactam is measured by comparing said light output to the light output of a standard reaction.
Claim 7
Dependent Claim
11 words
A method according to Claim 1, wherein said sample is milk.
Claim 8
Dependent Claim
12 words
A method according to Claim 1, wherein said sample is blood plasma.
Claim 9
Dependent Claim
29 words
A method according to Claim 1, wherein said reaction system produces a light output which is at least about four times that produced in the absence of penicillin G.
Claim 10
Dependent Claim
15 words
A method according to Claim 1, wherein said reaction system further comprises an oxidation catalyst.
Claim 11
Dependent Claim
28 words
A method according to Claim 9, wherein said reaction system further comprises a compound which poisons said oxidation catalyst in order to increase the sensitivity of said method.
Claim 12
Dependent Claim
43 words
A method according to Claim 10, wherein said compound is selected from the group consisting of an ascorbate salt, an azide salt, a mixture of an ascorbate salt and an azide salt, and a mixture of an azide salt and a cyanide salt.
Claim 13
Independent Claim
57 words
A method for enhancing the sensitivity of an assay reaction for the detection of a component of a chemiluminescent reaction involving a chemiluminescent compound, a peroxide source and optionally an oxidation catalyst, said component being one of said chemiluminescent compound, said peroxide source, and said oxidation catalyst, said method comprising including a beta-lactam in said assay reaction.
Claim 14
Independent Claim
57 words
A method for enhancing the sensitivity of an assay reaction for the detection of a component of a chemiluminescent reaction involving a chemiluminescent compound, a peroxide source and optionally an oxidation catalyst, said component being one of said chemiluminescent compound, said peroxide source, and said oxidation catalyst, said method comprising including a beta-lactam in said assay reaction.
Claim 15
Dependent Claim
15 words
A method according to Claim 12, wherein said component being detected is an oxidation catalyst.
Claim 16
Dependent Claim
13 words
A method according to Claim 13, wherein said oxidation catalyst is a peroxidase.
Claim 17
Dependent Claim
15 words
A method according to Claim 12, wherein said component being detected is a peroxide source.
Claim 18
Dependent Claim
13 words
A method according to Claim 15, wherein said peroxide source is hydrogen peroxide.
Claim 19
Dependent Claim
15 words
A method according to Claim 12, wherein said component being detected is said chemiluminescent compound.
Claim 20
Dependent Claim
68 words
An assay kit for detecting a component of a chemiluminescent reaction involving a chemiluminescent compound, a peroxide source and optionally an oxidation catalyst as reactants, said component being one of said chemiluminescent compound, said peroxide source and an oxidation catalyst, said assay kit comprising a) said reactants other than said component; and b) a beta-lactam; said reactants and beta-lactam in said kit being packaged in substantially stable form.
Claim 21
Dependent Claim
68 words
An assay kit for detecting a component of a chemiluminescent reaction involving a chemiluminescent compound, a peroxide source and optionally an oxidation catalyst as reactants, said component being one of said chemiluminescent compound, said peroxide source and an oxidation catalyst, said assay kit comprising a) said reactants other than said component; and b) a beta-lactam; said reactants and beta-lactam in said kit being packaged in substantially stable form.
Claim 22
Independent Claim
88 words
A method for the detection of a compound containing a beta-lactam ring in a sample suspected of containing a beta-lactam comprising the steps of a) combining together as a mixture i) a reaction system comprising a chemiluminescent compound, a peroxide source and a base, and ii) a predetermined portion of said sample, and b) measuring the light output of said mixture; wherein the chemiluminescent compound is selected from the group consisting of luminol, a luminol analog, isoluminol, an isoluminol analog, coumarin 2, sulforhodamine 101, umbelliferone, 4-methylumbelliferone and fluorescein.
Claim 23
Independent Claim
88 words
A method for the detection of a compound containing a beta-lactam ring in a sample suspected of containing a beta-lactam comprising the steps of a) combining together as a mixture i) a reaction system comprising a chemiluminescent compound, a peroxide source and a base, and ii) a predetermined portion of said sample, and b) measuring the light output of said mixture; wherein the chemiluminescent compound is selected from the group consisting of luminol, a luminol analog, isoluminol, an isoluminol analog, coumarin 2, sulforhodamine 101, umbelliferone, 4-methylumbelliferone and fluorescein.
Claim 24
Dependent Claim
15 words
A method according to Claim 19, wherein said reaction system further comprises an oxidation catalyst.
References Cited (Backward Citations)
No citation records indexed.
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