EP0155359A1
Expired / Lapsed
EP
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Method for identifying and characterizing organisms
Assignee: Individual
•
Filing: 1982-09-24
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Granted: —
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Expires: 2002-09-24
Abstract
Abstract A method of characterizing and/or identifying an unknown organism which comprises comparing the chromatographic pattern of restriction endonuclease-digested DNA from the organism, which digested DNA has been reassociated or hybridized with ribosomal RNA-information containing nucleic acid from a probe organism, with equivalent chromatographic patterns of at least two known different organism species.
Inventors
- 👤 John A. Webster, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 20:56
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/6813; C12Q1/6827; C12Q1/683
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
25 words
Detectably labeled DNA complementary to prokaryotic ribosomal RNA (rRNAcDNA) in substantially pure form, wherein said rRNAcDNA is a faithful copy of said ribosomal RNA (rRNA).
Claim 2
Dependent Claim
25 words
Detectably labeled DNA complementary to prokaryotic ribosomal RNA (rRNAcDNA) in substantially pure form, wherein said rRNAcDNA is a faithful copy of said ribosomal RNA (rRNA).
Claim 3
Dependent Claim
11 words
The rRNAcDNA of Claim 1 which is radiolabeled or metal labeled.
Claim 4
Dependent Claim
12 words
The rRNAcDNA of Claim 1 wherein said prokaryotic rRNA is bacterial rRNA.
Claim 5
Dependent Claim
21 words
The rRNAcDNA of Claim 1 which is substantially free of cDNA which is complementary to types of RNA other than rRNA.
Claim 6
Dependent Claim
12 words
The rRNAcDNA of Claim 1 which is substantially free of cellular components.
Claim 7
Dependent Claim
19 words
The rRNAcDNA of Claim 1 which has been obtained by reverse transcribing rRNA in the presence of a primer.
Claim 8
Dependent Claim
15 words
The rRNAcDNA of Claim 6 which has been obtained by using a DNA hydrolysate primer.
Claim 9
Dependent Claim
19 words
The rRNAcDNA of Claim 6 which has been obtained by reverse transcribing with reverse transcriptase from avian myeloblastosis virus.
References Cited (Backward Citations)
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