EP0154884A2
Expired / Lapsed
EP
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Nucleic acid-protein conjugate
Assignee: Molecular Diagnostics Inc
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Filing: 1985-02-27
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Granted: —
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Expires: 2005-02-27
Abstract
Abstract @ A protein is covalently coupled to at least one end of a nucleic acid which carries several labels. In an assay the protein will specifically recognize some component of a test system; in an immunoassay the protein can be Protein A which will recognize the Fc portion of IgG which is bound to an unknown antigen if present in the test sample.
Inventors
- 👤 Nanibhushan Dattagupta
- 👤 William J. Knowles
- 👤 Vincent T. Marchesi
- 👤 Donald M. Crothers
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 02:09
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/682; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; Y; Y10; Y10S; Y10S435/00; Y10S435/964; Y10S436/00; Y10S436/828
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
11 words
A protein covalently coupled to one end of a nucleic acid.
Claim 2
Dependent Claim
11 words
A protein covalently coupled to one end of a nucleic acid.
Claim 3
Dependent Claim
41 words
A coupled protein according to claim 1, characterized in that the nucleic acid is single or double stranded and the said nucleic acid is a deoxyribonucleic acid or a ribonucleic acid or an oligodeoxyribonucleotide or an oligoribonucleotide or a hybrid thereof.
Claim 4
Dependent Claim
24 words
A coupled protein according to claim 1 or 2, characterized in that the nucleic acid carries a Hg, SH or NH 2 functional group.
Claim 5
Dependent Claim
40 words
A coupled protein according to claims 1 - 3, characterized in that the protein is one which has an affinity for a) another protein, b) a specific nucleotide sequence of a nucleic acid, c) a hapten, or d) a ligand.
Claim 6
Dependent Claim
24 words
A coupled protein according to claims 1 - 4, characterized in that the nucleic acid carries at least one physically or chemically detectable label.
Claim 7
Dependent Claim
43 words
A coupled protein according to claims 1 - 5, characterized in that the label can be a fluorophore, a dye stuff, an antigen, a ligand, an enzyme, a substrate of an enzyme, a cofactor, a radioactive isotope, a carbohydrate or a combination thereof.
Claim 8
Independent Claim
33 words
A method for determination an analyte wherein the analyte is complexed with a specific binding compound, characterized in that the specific binding compound is linked to one end of a labeled nucleic acid.
Claim 9
Independent Claim
33 words
A method for determination an analyte wherein the analyte is complexed with a specific binding compound, characterized in that the specific binding compound is linked to one end of a labeled nucleic acid.
Claim 10
Dependent Claim
44 words
A method for determination an analyte according to claim 7, characterized in that the specific binding compound is a protein which has an affinity for a) another protein, b) a specific nucleotide sequence of a nucleic acid, c) a hapten, or d) a ligand.
Claim 11
Independent Claim
42 words
A method for preparation a protein which is covalently coupled to one and of a nucleic acid, characterized in that the nucleic acid is subjected to chemical or enzymatical treatment to introduce a functional group at one end capable for binding protein.
Claim 12
Independent Claim
42 words
A method for preparation a protein which is covalently coupled to one and of a nucleic acid, characterized in that the nucleic acid is subjected to chemical or enzymatical treatment to introduce a functional group at one end capable for binding protein.
Claim 13
Independent Claim
53 words
A method for preparation of a protein which is covalently coupled to one end of a nucleic acid, characterized in that the nucleic acid carries a ribose moiety at one end which is oxidized to form pendent aldehyde groups which are capable for binding proteins via a Schiff-Base which is reduced if necessary.
Claim 14
Independent Claim
53 words
A method for preparation of a protein which is covalently coupled to one end of a nucleic acid, characterized in that the nucleic acid carries a ribose moiety at one end which is oxidized to form pendent aldehyde groups which are capable for binding proteins via a Schiff-Base which is reduced if necessary.
Claim 15
Dependent Claim
41 words
A method for preparation of a protein which is covalently coupled to one end of a nucleic acid according to claim 9 and 10, characterized in that the nucleic acid is labeled with at least one physically or chemically detectable label.
References Cited (Backward Citations)
No citation records indexed.
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