EP0152613A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Novel promoter
Assignee: KH Neochem Co Ltd
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Filing: 1984-12-21
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Granted: —
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Expires: 2004-12-21
Abstract
Abstract Disclosed is a novel promoter wherein a 5' flanking region of a promoter (referred to as promoter A) is replaced by a 5' flanking region of another promoter (referred to as promoter B) or a chemically synthesized DNA fragment. The promoter is constructed by replacing a region existing upstream from the "-35" region of promoter A by the "-35" region of promoter B or a chemically synthesized DNA fragment.
Inventors
- 👤 Tatsunari Nishi
- 👤 Akiko Saito
- 👤 Seiga Itoh
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 14:16
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/57; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/14; Y10S930/142
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
36 words
A promoter wherein a 5' flanking region of a promoter (referred to as promoter A) is replaced with a 5' flanking region of another promoter (referred to as promoter B) or a chemically synthesized DNA fragment.
Claim 2
Dependent Claim
36 words
A promoter wherein a 5' flanking region of a promoter (referred to as promoter A) is replaced with a 5' flanking region of another promoter (referred to as promoter B) or a chemically synthesized DNA fragment.
Claim 3
Dependent Claim
28 words
The promoter according to claim 1, wherein the 5' flanking region is replaced using a restriction site existing around or upstream from the "-35" region of promoter A.
Claim 4
Dependent Claim
40 words
The promoter according to claim 1, wherein the 5' flanking region of promoter B or a chemically synthesized DNA fragment is inserted using a suitable restriction site existing in the "-35" region or the 5' flanking region of promoter A.
Claim 5
Dependent Claim
39 words
The promoter according to claim 2 or 3, wherein the 5' flanking region of promoter B or a chemically synthesized DNA fragment is inserted after removing the 5' flanking region of promoter A using an exonuclease or the like.
Claim 6
Dependent Claim
21 words
The promoter according to one of claims 1 - 4, wherein promoter A and promoter B are derived from Escherichia coli.
Claim 7
Dependent Claim
32 words
The promoter according to claim 5, wherein the 5' flanking region of promoter B or the chemically synthesized DNA fragment is abundant with AT and has the effect of strengthening transcription activity.
Claim 8
Dependent Claim
37 words
The promoter according to claim 5, wherein promoter is the P L promoter of the λ phage, the P R promoter of the λ phage, the ribosomal RNA promoter (rrn) or the promoter of the lipoprotein gene.
Claim 9
Dependent Claim
43 words
The promoter according to claim 5, wherein promoter A is the promoter of the tryptophan (trp) operon (trp promoter), the promoter of the lactose (lac) operon (lac promoter) or the tac promoter which is a hybrid promoter of trp promoter and lac promoter.
Claim 10
Dependent Claim
25 words
The promoter according to claim 5, wherein promoter A is the trp promoter or the tac promoter and promoter B is the P L promoter.
Claim 11
Dependent Claim
28 words
A recombinant plasmid which is replicable and contains a foreign gene the expression of which is controlled by a promoter described in one of claims 1 - 9.
Claim 12
Dependent Claim
10 words
A microorganism containing the recombinant plasmid claimed in claim 10.
Claim 13
Dependent Claim
18 words
A microorganism according to claim 11, which is Escherichia coli IGHA2, Escherichia coli IGHB3 or Escherichia coli ILEC1.
References Cited (Backward Citations)
No citation records indexed.
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