EP0150735A2
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EP
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Protein composition exhibiting coagulation activity and method for the preparation thereof
Assignee: Novo Nordisk AS
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Filing: 1985-01-11
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Granted: —
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Expires: 2005-01-11
Abstract
Abstract Methods and compositions are provided for recombinant DNA production of Factor VIIIC and truncated derivatives thereof. Based on amino acid sequences, probes are developed for Isolating messenger RNA and/or chromosomal DNA encoding for Factor VIIIC. The Factor VIIIC gene in its entirety or encoding for a fragment thereof is then used for expression of Factor VIIIC in a host. The bacteriophage λFVIII23D containing the 14.43kb EcoRl fragment was deposited at the A.T.C.C. on January 4,1984 and given Accession No. 40094.
Inventors
- 👤 George Kuo
- 👤 Frank R. Masiarz
- 👤 Martha Truett
- 👤 Pablo Valenzuela
- 👤 Mirella Ezban Rasmussen
- 👤 Jennifer Favaloro
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 20:57
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; A; A61; A61P; A61P7/00; A61P7/04; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; C07K16/00; C07K16/18; C07K16/36; A61K; A61K38/00
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
30 words
A protein composition substantially free of other proteins having a complex of an 80kd and 77kd protein calcium bridged with a 92.5kd protein exhibiting a biological activity of Factor VIIIC.
Claim 2
Dependent Claim
30 words
A protein composition substantially free of other proteins having a complex of an 80kd and 77kd protein calcium bridged with a 92.5kd protein exhibiting a biological activity of Factor VIIIC.
Claim 3
Dependent Claim
38 words
A human protein composition comprising primarily from 1 to 2 polypeptides of from about 67 to 80kd and having an amino acid sequence: in the N-terminal half of said polypeptide and having a biological activity of Factor VIIIC.
Claim 4
Dependent Claim
38 words
A human protein composition comprising primarily from 1 to 2 polypeptides of from about 67 to 80kd and having an amino acid sequence: in the N-terminal half of said polypeptide and having a biological activity of Factor VIIIC.
Claim 5
Dependent Claim
31 words
A human protein composition comprising primarily a polypeptide of about 92.5kd having an amino acid sequence: in the N-terminal half of said polypeptide and having a biological activity of Factor VIIIC.
Claim 6
Dependent Claim
31 words
A human protein composition comprising primarily a polypeptide of about 92.5kd having an amino acid sequence: in the N-terminal half of said polypeptide and having a biological activity of Factor VIIIC.
Claim 7
Dependent Claim
14 words
Monoclonal antibodies prepared with an immunogen comprising a protein composition according to Claim 1.
Claim 8
Dependent Claim
14 words
Monoclonal antibodies prepared with an immunogen comprising a protein composition according to Claim 2.
Claim 9
Dependent Claim
14 words
Monoclonal antibodies prepared with an immunogen comprising a protein composition according to Claim 3.
Claim 10
Dependent Claim
7 words
A complex probe having the following formula:
Claim 11
Dependent Claim
7 words
A complex probe having the following formula:
Claim 12
Dependent Claim
45 words
A method for detecting the presence of a naturally occurring polynucleotide sequence encoding for at least a portion of Factor VIIIC, said method comprising: hybridizing naturally occurring polynucleotide in single-stranded form with a probe according to Claim 7; and isolating polynucleotide duplexing with said probe.
Claim 13
Dependent Claim
11 words
A method according to Claim 8, wherein said polynucleotide is DNA.
Claim 14
Dependent Claim
11 words
A method according to Claim 8, wherein said polynucleotide is RNA.
Claim 15
Dependent Claim
30 words
A DNA sequence of at least about 200bp having an open reading frame throughout said sequence and encoding for at least a portion of human Factor VIIIC or precursor thereof.
Claim 16
Dependent Claim
30 words
A DNA sequence of at least about 200bp having an open reading frame throughout said sequence and encoding for at least a portion of human Factor VIIIC or precursor thereof.
Claim 17
Dependent Claim
17 words
A DNA sequence according to Claim 11, including the DNA sequence set forth in the Experimental section.
Claim 18
Dependent Claim
11 words
A DNA sequence encoding for a subunit of human Factor VIIIC.
Claim 19
Dependent Claim
11 words
A DNA sequence encoding for a subunit of human Factor VIIIC.
Claim 20
Dependent Claim
15 words
A DNA sequence according to Claim 13 encoding for the 80kd peptide of Factor VIIIC.
Claim 21
Dependent Claim
15 words
A DNA sequence according to Claim 13 encoding for the 77kd peptide of Factor VIIIC.
Claim 22
Dependent Claim
39 words
An extrachromosomal element having a replication system compatible with a host cell capable of growth in culture and a DNA sequence of at least about 200bp encoding for at least a portion of human Factor VIIIC or precursor thereof.
Claim 23
Dependent Claim
39 words
An extrachromosomal element having a replication system compatible with a host cell capable of growth in culture and a DNA sequence of at least about 200bp encoding for at least a portion of human Factor VIIIC or precursor thereof.
Claim 24
Dependent Claim
19 words
An extrachromosomal element according to Claim 16, wherein said DNA sequence encodes for the 80kd peptide of Factor VIIIC.
Claim 25
Dependent Claim
19 words
An extrachromosomal element according to Claim 13, wherein said DNA sequence encodes for the 77kd peptide of Factor VIIIC.
Claim 26
Dependent Claim
29 words
An extrachromosomal element according to Claim 13, including transcriptional and translational regulatory signals compatible with said host in juxtaposition to said DNA sequence for expression of said DNA sequence.
Claim 27
Dependent Claim
14 words
An extrachromosomal element according to Claim 13, wherein said host is a unicellular microorganism.
Claim 28
Dependent Claim
14 words
An extrachromosomal element according to Claim 13, wherein said host is a mammalian cell.
Claim 29
Dependent Claim
32 words
A DNA sequence of from about 10 to 25kbp derived from a mammalian genome coding for at least a portion of Factor VIIIC and hybridizing with a probe according to Claim 7.
Claim 30
Dependent Claim
18 words
A DNA construct comprising a DNA sequence according to Claim 22 joined to a unicellular microorganism cloning vector.
References Cited (Backward Citations)
No citation records indexed.
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