EP0149594A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Enzymatic coupling of n-formyl amino acids and/or peptide residues
Assignee: Monsanto Co
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Filing: 1985-01-15
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Granted: —
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Expires: 2005-01-15
Abstract
Abstract An improved process for enzyme mediated peptide bond formation is disclosed. The process embraces the discovery that the formyl group can be utilized to selectively protect the amino group of one substrate from undergoing peptide bond formation.
Inventors
- 👤 John Joseph Likos
- 👤 George Irvin Glover
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 10:07
CPC Classifications
C; C07; C07K; C07K5/00; C07K5/04; C07K5/06; C07K5/06104; C07K5/06113; C07K5/06121; C07K5/0613; C12; C12P; C12P21/00; C12P21/02
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
54 words
In a process in which anenzyme is used to mediate peptide bond formation between two substrates selected from the group consisting of amino acids and peptides, the improvement comprising selectively protecting the amino group of one substrate from undergoing said bond formation by using either the N-formyl amino acid derivative or N-formyl peptide derivative.
Claim 2
Dependent Claim
54 words
In a process in which anenzyme is used to mediate peptide bond formation between two substrates selected from the group consisting of amino acids and peptides, the improvement comprising selectively protecting the amino group of one substrate from undergoing said bond formation by using either the N-formyl amino acid derivative or N-formyl peptide derivative.
Claim 3
Dependent Claim
15 words
The process of Claim 1 in which the other substrate is an alkyl ester derivative.
Claim 4
Dependent Claim
12 words
The process of Claim 1 in which the enzyme is a protease.
Claim 5
Dependent Claim
14 words
The process of Claim 2 in which the ester is a lower alkyl ester.
Claim 6
Dependent Claim
43 words
A process for producing N-formyl-a-L-aspartyl-L-phenylalanine alkyl ester which comprises reacting N-formyl-L-aspartic acid with L-phenylalanine alkyl ester in the presence of an enzyme known to mediate peptide bond formation between L-aspartic acid and L-phenylalanine and then acidifying the mixture to yield the dipeptide product.
Claim 7
Dependent Claim
43 words
A process for producing N-formyl-a-L-aspartyl-L-phenylalanine alkyl ester which comprises reacting N-formyl-L-aspartic acid with L-phenylalanine alkyl ester in the presence of an enzyme known to mediate peptide bond formation between L-aspartic acid and L-phenylalanine and then acidifying the mixture to yield the dipeptide product.
Claim 8
Dependent Claim
15 words
The process of Claim 5 in which the L-phenylalanine ester is a lower alkyl ester.
Claim 9
Dependent Claim
14 words
The process of Claim 5 in which the L-phenylalanine ester is the methyl ester.
Claim 10
Dependent Claim
11 words
The process of Claim 5 in which the enzyme is thermolysin.
Claim 11
Dependent Claim
51 words
A process for producing a-L-aspartyl-L-phenylalanine methyl ester which comprises reacting N-formyl-L-aspartic acid with L-phenylalanine methyl ester in the presence of an enzyme known to mediate peptide bond formation between L-aspartic acid and L-phenylalanine methyl ester and then subjecting the N-formyl dipeptide to mild hydrolysis to remove the amino protecting formyl group.
Claim 12
Dependent Claim
51 words
A process for producing a-L-aspartyl-L-phenylalanine methyl ester which comprises reacting N-formyl-L-aspartic acid with L-phenylalanine methyl ester in the presence of an enzyme known to mediate peptide bond formation between L-aspartic acid and L-phenylalanine methyl ester and then subjecting the N-formyl dipeptide to mild hydrolysis to remove the amino protecting formyl group.
Claim 13
Dependent Claim
11 words
The process of Claim 9 in which the enzyme is thermolysin.
References Cited (Backward Citations)
No citation records indexed.
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