EP0149241A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
DNA base sequence containing regions involved in the production and secretion of a protein, recombinant DNA including the whole or a part of the DNA base sequence, and method of producing proteins by use of the recombinant DNA
Assignee: National Institute of Advanced Industrial Science and Technology
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Filing: 1984-12-27
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Granted: —
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Expires: 2004-12-27
Abstract
Abstract This invention relates to a DNA base sequence containing regions involved in the production and secretion of a protein, a recombinant DNA including the whole or a part of said DNA base sequence, and a method of producing proteins which comprises introducing said recombinant DNA into a microorganism, culturing this microorganism and thereby causing the protein to be extracellularly secreted in large amounts, and then recovering the protein.
Inventors
- 👤 Yoshio Furutani
- 👤 Hiroaki Shimada
- 👤 Izumi Mita
- 👤 Kazuaki Manabe
- 👤 Masaru Honjo
- 👤 Noboru Tomioka
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 22:40
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/75; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; C07; C07K; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/036
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
53 words
A DNA base sequence containing one or more regions selected from the group consisting of the promoter region, the ribosome binding region, the region involved in the extracellular secretion of the protein, the structural gene and the terminator region, all being of the neutral protease gene of a bacterium of the genus Bacillus.
Claim 2
Dependent Claim
53 words
A DNA base sequence containing one or more regions selected from the group consisting of the promoter region, the ribosome binding region, the region involved in the extracellular secretion of the protein, the structural gene and the terminator region, all being of the neutral protease gene of a bacterium of the genus Bacillus.
Claim 3
Dependent Claim
28 words
A DNA base sequence as claimed in claim 1 wherein said bacterium of the genus Bacillus is Bacillus amyloliquefaciens, B. subtilis, B. licheniformis, B. cereus or B. megaterium.
Claim 4
Dependent Claim
44 words
A DNA base sequence as claimed in claim 1 wherein said bacterium of the genus Bacterium is B. amyloliquefaciens and one of the strands comprises bases arranged in the following order: where A, T, G and C represent adenine, thymine, guanine and cytosine, respectively.
Claim 5
Dependent Claim
28 words
A DNA base sequence obtained by subjecting a part of the DNA base sequence of claim 3 to base substitution, deletion, insertion or transposition without impairing its functions.
Claim 6
Dependent Claim
27 words
A recombinant DNA comprising vector DNA combined with a DNA fragment including the whole or a part of the DNA base sequence of claim 3 or 4.
Claim 7
Dependent Claim
22 words
A recombinant DNA as claimed in claim 5 wherein the structural gene for the protein comprises the structural gene for neutral protease.
Claim 8
Dependent Claim
25 words
A recombinant DNA as claimed in claim 5 wherein the vector moiety thereof comprises a plasmid or phage that can be retained within bacterial cells.
Claim 9
Dependent Claim
25 words
A recombinant DNA as claimed in claim 6 wherein the vector moiety thereof comprises a plasmid or phage that can be retained within bacterial cells.
Claim 10
Dependent Claim
24 words
A recombinant DNA as claimed in claim 7 or 8 wherein the vector moiety thereof comprises plasmid pUB110, plasmid pBR322 or a derivative thereof.
Claim 11
Dependent Claim
24 words
A recombinant DNA as claimed in claim 7 or 8 wherein the vector portion thereof comprises λ phage, M13 phage, phage or Ø105 phage.
Claim 12
Dependent Claim
34 words
A method of producing proteins which comprises transforming a bacterium with a recombinant DNA as claimed in claim 5 and then culturing said transformed strain to yield the protein corresponding to said recombinant DNA.
References Cited (Backward Citations)
No citation records indexed.
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