EP0148552A1
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EP
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Streptomyces expression sequence and methods for expressing DNA sequences in streptomyces
Assignee: Biogen Inc
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Filing: 1984-06-04
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Granted: —
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Expires: 2004-06-04
Abstract
Abstract Streptomyces expression systems and methods for expressing foreign DNA sequences in Streptomyces characterized by at least one promoter selected from the group consisting of the promoters of Streptomyces aminoglycoside phosphotransferases. These expression systems and methods enable the expression of eukaryotic and viral DNA sequences in Streptomyces and the production of various pharmaceutical, veterinary and industrial products in those hosts.
Inventors
- 👤 Charles J. Thompson
- 👤 Gary S. Gray
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 12:53
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/76; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; C12N9/00; C12N9/10; C12N9/12; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
29 words
A Streptomyces expression system for expressing foreign DNA sequences in Streptomyces characterized by at least one promoter selected from the group consisting of the promoters of Streptomyces aminoglycoside phosphotransferases.
Claim 2
Dependent Claim
29 words
A Streptomyces expression system for expressing foreign DNA sequences in Streptomyces characterized by at least one promoter selected from the group consisting of the promoters of Streptomyces aminoglycoside phosphotransferases.
Claim 3
Dependent Claim
14 words
The expression system of claim 1, wherein said Streptomyces aminoglycoside phosphotransferase is neomycin phosphotransferase.
Claim 4
Dependent Claim
25 words
The expression system of claim 1 or 2, wherein said foreign DNA sequence is selected from the group consisting of eukaryotic and viral DNA sequences.
Claim 5
Dependent Claim
50 words
The expression system of claim 3, wherein said foreign DNA sequence is selected from the group consisting of DNA sequences encoding the human and animal leukocyte, fibroblast and immune interferons, human insulin, animal and human growth and other hormones, human blood factors, hepatitis B viral antigens and FMD viral antigens.
Claim 6
Independent Claim
50 words
A method of expressing a foreign DNA sequence in Streptomyces characterized by the step of operatively linking said foreign DNA sequence to a Streptomyces expression control sequence, said expression control sequence being characterized by at least one promoter selected from the group consisting of the promoters of Streptomyces aminoglycoside phosphotransferases.
Claim 7
Independent Claim
50 words
A method of expressing a foreign DNA sequence in Streptomyces characterized by the step of operatively linking said foreign DNA sequence to a Streptomyces expression control sequence, said expression control sequence being characterized by at least one promoter selected from the group consisting of the promoters of Streptomyces aminoglycoside phosphotransferases.
Claim 8
Dependent Claim
13 words
The method of claim 5, wherein said Streptomyces aminoglycoside phosphotransferase is neomycin phosphotransferase.
Claim 9
Dependent Claim
24 words
The method of claim 5 or 6, wherein said foreign DNA sequence is selected from the group consisting of eukaryotic and viral DNA sequences.
Claim 10
Independent Claim
97 words
The method of any one of claims 5 to 7, wherein said foreign DNA sequence is operatively linked to said Streptomyces expression control sequence through a DNA sequence encoding at least a part of the signal sequence of a protein or polypeptide secreted from Streptomyces or at least part of another signal sequence functional in Streptomyces, said DNA sequence encoding the signal sequence being expressed together with said foreign DNA sequence under the control of said Streptomyces expression control sequence to afford excretion and maturation of the protein or polypeptide coded for by the foreign DNA sequence.
Claim 11
Independent Claim
78 words
The method of any one of claims 5 to 7, wherein said foreign DNA sequence is operatively linked to said Streptomyces expression control sequence through a DNA sequence encoding a part of another protein or polypeptide or a part of the signal sequence and coding sequence of another protein or polypeptide, said DNA sequence being expressed together with said foreign DNA sequence under the control of said Streptomyces expression control sequence to produce a fused protein or polypeptide.
Claim 12
Independent Claim
35 words
A method of amplifying the production of a streptomyces-derived antibiotic or other product characterized by the step of employing at least one promoter selected from the group consisting of the promoters of Streptomyces aminoglycoside phosphotransferases.
Claim 13
Independent Claim
35 words
A method of amplifying the production of a streptomyces-derived antibiotic or other product characterized by the step of employing at least one promoter selected from the group consisting of the promoters of Streptomyces aminoglycoside phosphotransferases.
Claim 14
Dependent Claim
12 words
The method of claim 10, wherein said aminoglycoside phosphotransferase is neomycin phosphotransferase.
Claim 15
Dependent Claim
12 words
The method of claim 10, wherein said aminoglycoside phosphotransferase is kanamycin phosphotransferase.
References Cited (Backward Citations)
EP0077650A2
Cited
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