EP0146929A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Microorganism of the genus Serratia containing a hybrid plasmid and process for preparing the same
Assignee: Tanabe Pharma Corp
•
Filing: 1984-12-20
•
Granted: —
•
Expires: 2004-12-20
Abstract
Abstract A novel microorganism of the genus Serratia containing a hybrid plasmid prepared by joining a vector plasmid to a chromosomal fragment encoding enzymes of proline synthesis which is obtained from a chromosomal DNA of a microorganism of the genus Serratia having L-proline-producing activity. A method for producing L-proline by using said novel microorganism is also disclosed.
Inventors
- 👤 Masahiko Kisumi
- 👤 Masaki Sugiura
- 👤 Tsutomu Takagi
- 👤 Yuji Imai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 16:16
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/24; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
43 words
A microorganism of the genus Serratia containing a hybrid plasmid prepared by joining a vector plasmid to a chromosomal DNA fragment encoding enzymes of proline synthesis which is obtained from a chromosomal DNA of a microorganism of the genus Serratia having L-proline-producing activity.
Claim 2
Dependent Claim
43 words
A microorganism of the genus Serratia containing a hybrid plasmid prepared by joining a vector plasmid to a chromosomal DNA fragment encoding enzymes of proline synthesis which is obtained from a chromosomal DNA of a microorganism of the genus Serratia having L-proline-producing activity.
Claim 3
Dependent Claim
13 words
The microorganism of claim 1, which is Serratia marcescens containing the hybrid plasmid.
Claim 4
Dependent Claim
40 words
The microorganism of claim 2, wherein the chromosomal DNA fragment is a deoxyribcnucleic acid carrying the genes for Γ-glutamyl kinase and δ=glutamyl phosphate reductase or a deoxyribonucleic acid carrying the genes for δ-glutamyl kinase, δ=glutamyl phosphate reductase and pyrroline-5-carboxylate reductase.
Claim 5
Dependent Claim
30 words
The microorganism of claim 2, wherein the chromosomal DNA fragment is a deoxyribonucleic acid carrying the genes for δ=glutamyl kinase and δ-glutamyl phosphate reductase, and the vector plasmid is pKPl155.
Claim 6
Dependent Claim
32 words
The microorganism of claim 2, wherein the chromosomal DNA.fragment is a deoxyribonucleic acid carrying the genes for δ-glutamyl kinase, δ-glutamyl phosphate reductase and pyrroline-5-carboxylate reductase, and the vector plasmid is pKP 1155.
Claim 7
Dependent Claim
43 words
The microorganism of claim 2, wherein the Serratia marcescens containing the hybrid plasmid is a member selected from the group consisting of Serratia marcescens TA5006 (FERM BP-649), Serratia marcescens TA5007 (FERM BP-650), Serratia marcescens TA5008 (FERM BP-651) and Serratia marcescens TA5009 (FERM BP-652).
Claim 8
Independent Claim
68 words
A method for producing L-proline which comprises cultivating a microorganism of the genus Serratia containing a hybrid plasmid prepared by joining a vector plasmid to a chromosomal DNA fragment encoding enzymes of proline synthesis which is obtained from a chromosomal DNA of a microorganism of the genus Serratia having L-proline-producing activity, in a nutrient medium to produce and accumulate L-proline in the medium, and recovering the L-proline therefrom.
Claim 9
Independent Claim
68 words
A method for producing L-proline which comprises cultivating a microorganism of the genus Serratia containing a hybrid plasmid prepared by joining a vector plasmid to a chromosomal DNA fragment encoding enzymes of proline synthesis which is obtained from a chromosomal DNA of a microorganism of the genus Serratia having L-proline-producing activity, in a nutrient medium to produce and accumulate L-proline in the medium, and recovering the L-proline therefrom.
Claim 10
Dependent Claim
14 words
The method of claim 7, wherein Serratia marcescens having the hybrid plasmid is cultivated.
Claim 11
Dependent Claim
43 words
The method of claim 8, wherein the Serratia marcescens containng the hybrid plasmid is a member selected from the group consisting of Serratia marcescens TA5006 (FERM BP-649), Serratia marcescens TA5007 (FERM BP-650), Serratia marcescens TA5008 (FERM BP-651) and Serratia marcescens TA5009 (FERM BP-652).
Claim 12
Dependent Claim
23 words
The method of claim 8, wherein the cultivation is carried out at 25 to 40°C for 2 to 6 days under aerobic conditions.
Claim 13
Dependent Claim
48 words
The method of claim 8, wherein the nutrient medium contains 10 to 20 w/v % of a carbon source, 1 to 5 w/v % of a nitrogen source and 0 to 1 w/v % of an organic nutrient, and the pH of said medium is 6 to 8.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.