Patent US1390596A successfully refreshed from Google Patents.
EP0145111A1
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DNA sequence coding for a plant toxin of the ricin type, or a portion thereof
Assignee: Imperial Chemical Industries Ltd
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Filing: 1984-07-13
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Granted: —
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Expires: 2004-07-13
Abstract
Abstract DNA comprising a nucleotide sequence coding for at least a portion of the precursor polypeptide of ricin or a similar plant toxin. Recombinant DNA molecules containing such DNA as an insert, and genetically-modified host microorganisms containing such recombinant DNA molecules.
Inventors
- 👤 John Michael Lord
- 👤 Lynne Margaret Roberts
- 👤 Francis Ian Lamb
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 14:35
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/415
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
32 words
DNA comprising a nucleotide sequence coding for at least a substantial portion of a plant toxin of the ricin-type or a mutant thereof, characterised in that it is biologically pure and homogeneous.
Claim 2
Dependent Claim
32 words
DNA comprising a nucleotide sequence coding for at least a substantial portion of a plant toxin of the ricin-type or a mutant thereof, characterised in that it is biologically pure and homogeneous.
Claim 3
Dependent Claim
24 words
DNA according to claim 1, characterised in that the nucleotide sequence codes for the A chain or the B chain of the mature toxin.
Claim 4
Dependent Claim
31 words
DNA according to claim 1, 2 or 3, characterised in that the nucleotide sequence codes for a mutant in which the galactose binding site or sites have been removed or inactivated.
Claim 5
Dependent Claim
15 words
A sample of DNA including at least a substantial portion of the following nucleotide sequence:
Claim 6
Dependent Claim
15 words
A sample of DNA including at least a substantial portion of the following nucleotide sequence:
Claim 7
Dependent Claim
22 words
or at least a portion of a nucleotide sequence that is equivalent thereto by virtue of degeneracy of the genetic 10 code.
Claim 8
Dependent Claim
22 words
or at least a portion of a nucleotide sequence that is equivalent thereto by virtue of degeneracy of the genetic 10 code.
Claim 9
Dependent Claim
37 words
A sample of DNA including at least a substantial portion of the following nucleotide sequence: 10 or at least a portion of a nucleotide sequence which is equivalent thereto by virtue of degeneracy of the genetic code.
Claim 10
Dependent Claim
37 words
A sample of DNA including at least a substantial portion of the following nucleotide sequence: 10 or at least a portion of a nucleotide sequence which is equivalent thereto by virtue of degeneracy of the genetic code.
Claim 11
Dependent Claim
36 words
A sample of DNA including at least a substantial portion of the following nucleotide sequence: or at least a portion of a nucleotide sequence which is equivalent thereto by virtue of degeneracy of the genetic code.
Claim 12
Dependent Claim
36 words
A sample of DNA including at least a substantial portion of the following nucleotide sequence: or at least a portion of a nucleotide sequence which is equivalent thereto by virtue of degeneracy of the genetic code.
Claim 13
Dependent Claim
21 words
A recombinant DNA molecule, characterised in that it includes as an insert a nucleotide sequence as defined in any preceding claim.
Claim 14
Dependent Claim
27 words
A recombinant DNA molecule according to claim 7, characterised in that said insert has been introduced into a cloning vector which is a plasmid or a bacteriophage.
Claim 15
Dependent Claim
16 words
A modified host cell containing a recombinant DNA molecule according to claim 7 or claim 8.
Claim 16
Dependent Claim
29 words
A modified host cell according to claim 9. characterised in that the host is a plant cell, an animal cell, a Gram-negative bacterium, a Gram-positive bacterium, or a yeast.
Claim 17
Dependent Claim
29 words
A modified host microorganism according to claim 10, characterised in that the host is a Gram-negative bacterium, which is any of E.coli, Meth y lophilus methylotropus, or Alcaligenes eutrophus.
Claim 18
Dependent Claim
25 words
A modified host microorganism according to claim 10, characterised in that the host is a Gram-positive bacterium which is any of Streptomyces, Bacillus or Arthrobacter.
Claim 19
Dependent Claim
20 words
A modified host microorganism according to claim 10, characterised in that the host is a yeast which is Saccharomyces cervisiae.
Claim 20
Dependent Claim
24 words
A modified host microorganism according to claim 11, wherein the cloning vector is any suitable plasmid selected from pBR322, pAT153, pUC8, pGS15 or pMB9.
Claim 21
Dependent Claim
17 words
A modified host microorganism according to claim 12, wherein the cloning vector is the plasmid pUC6 .
Claim 22
Dependent Claim
19 words
A modified host microorganism according to claim 13, when the cloning vector is pMA91, pMA230, YRp7, pLC544, and YEp6.
Claim 23
Dependent Claim
48 words
Method of preparing a biologically-pure sample of a cDNA sequence coding for a precursor,or a portion thereof, of a plant toxin of the ricin type, characterised by isolating mRNA from the tissue of a plant which produces such a toxin, and synthesising cDNA from this by reverse transcription.
Claim 24
Dependent Claim
48 words
Method of preparing a biologically-pure sample of a cDNA sequence coding for a precursor,or a portion thereof, of a plant toxin of the ricin type, characterised by isolating mRNA from the tissue of a plant which produces such a toxin, and synthesising cDNA from this by reverse transcription.
Claim 25
Dependent Claim
21 words
A method of obtaining a recombinant DNA molecule by inserting a double-stranded cDNA according to claim 17 into a cloning vector.
Claim 26
Dependent Claim
24 words
A method of obtaining a genetically-modified host characterised in that a recombinant DNA molecule according to claim 18 is introduced into a host microorganism.
References Cited (Backward Citations)
No citation records indexed.
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