EP0142268A1
Expired / Lapsed
EP
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Saccharomyces cerevisiae possessing gene coding for interleukin-2 polypeptide and method for producing interleukin-2 using the yeast
Assignee: Ajinomoto Co Inc
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Filing: 1984-10-11
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Granted: —
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Expires: 2004-10-11
Abstract
Abstract @ There is disolved a cell of Saccharomyces cerevisiae transformed with a recontinant DNA comprising a gene coding for a polypeptide possessing the activity of interieukin-2 and a vector DNA capable of propagating in said cell, the coding sequence of said gene being located at a position downstream of a promoter sequence.
Inventors
- 👤 Tadatsugu Taniguchi
- 👤 Hiroshi Matsui
- 👤 Junji Hamuro
- 👤 Takaaki Sato
- 👤 Konosuke Sano
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 23:44
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/54; C07K14/55; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
48 words
A cell of Saccharomyces cerevisiae transformed with a recombinant DNA comprising a gene coding for a polypeptide possessing the activity of interleukin-2 and a vector DNA capable of propagating in said cell, the coding sequence of said gene being located at a position downstream of a promoter sequence.
Claim 2
Dependent Claim
48 words
A cell of Saccharomyces cerevisiae transformed with a recombinant DNA comprising a gene coding for a polypeptide possessing the activity of interleukin-2 and a vector DNA capable of propagating in said cell, the coding sequence of said gene being located at a position downstream of a promoter sequence.
Claim 3
Dependent Claim
21 words
The cell of claim 1, wherien said gene is prepared with a messenger RNA produced by an interleukin-2 producing mammalian cell.
Claim 4
Dependent Claim
21 words
The cell of claim 2, wherein said mammalian cell is a human T-lymphocyte, a transformed human T-lymphocyte or a T-cell hybridoma.
Claim 5
Dependent Claim
24 words
. The cell of claim 2, wherein said messenger RNA is obtainable as a sediment of 11 to 12S of sucrose density gradient centrifugation.
Claim 6
Dependent Claim
31 words
The cell of claim 2, wherein said gene has sites cleaved with restriction endonuclease in the order of Bst NI, Xba I and Bst NI from 5'-terminus of the coding sequence.
Claim 7
Dependent Claim
38 words
6,. The cell of claim 1, wherein said gene has sites cleaved with restriction endonuclease in the order of Dde I, Hinf I, Bst NI, Xba I, Bst NI and Sau 3A from 5'-terminus of the coding sequence.
Claim 8
Dependent Claim
16 words
The cell of claim 1, wherein said gene has the base sequence shown in Figure 2(a).
Claim 9
Dependent Claim
22 words
The cell of claim 4, wherein the base sequence of said gene corresponds to Amino Acid Sequence II in Figure 2 (b).
Claim 10
Dependent Claim
22 words
The cell of claim 4, wherein the base sequence of said gene corresponds to Amino Acid Sequence III in Figure 2 (b).
Claim 11
Independent Claim
71 words
A method for producing interleukin-2 which comprises culturing Saccharomyces cerevisiae in culture medium, transformed with a recombination DNA to produce interleukin-2 and recovering the produced interleukin-2, said recombinant DNA comprising a gene coded for a polypeptide which possesses the activity of interleukin-2 and a vector DNA which is capable of replicating in said cell, and the coding sequence of said gene being located at a position downstream of a promoter sequence.
Claim 12
Independent Claim
71 words
A method for producing interleukin-2 which comprises culturing Saccharomyces cerevisiae in culture medium, transformed with a recombination DNA to produce interleukin-2 and recovering the produced interleukin-2, said recombinant DNA comprising a gene coded for a polypeptide which possesses the activity of interleukin-2 and a vector DNA which is capable of replicating in said cell, and the coding sequence of said gene being located at a position downstream of a promoter sequence.
Claim 13
Dependent Claim
20 words
A method according to claim 11, wherein the Saccharomyces cerevisiae is that according to any of claims 1 to 9.
Claim 14
Dependent Claim
4 words
Saccharomyces cerevisiae FERM-P 7303.
Claim 15
Dependent Claim
4 words
Saccharomyces cerevisiae FERM-P 7303.
Claim 16
Dependent Claim
4 words
Saccharomyces cerevisiae FERM-P 7304
Claim 17
Dependent Claim
4 words
Saccharomyces cerevisiae FERM-P 7304
References Cited (Backward Citations)
EP0091539A1
Cited
EP0775771A1
Cited
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