Patent US3439935A successfully refreshed from Google Patents.
EP0141627A2
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EP
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Carrier film for immobilizing protein and its production, and protein immobilized thereby and the use thereof
Assignee: SUSUMU INDUSTRIAL Co Ltd
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Filing: 1984-10-25
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Granted: —
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Expires: 2004-10-25
Abstract
Abstract A carrier film for immobilizing protein on which non-specific adsorption of protein is suppressed comprises a polymeric film at least one surface of which is incinerated using a low temperature plasma of oxygen or oxygen plus an inert gas.
Inventors
- 👤 Kazuo C/O Susumu Industrial Co. Ltd. Horiguchi
- 👤 Junko Uo
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 02:09
CPC Classifications
B; B29; B29C; B29C59/00; B29C59/14; C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/089; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
32 words
A carrier film for immobilizing protein comprising a polymeric film at least one surface of which has been incinerated using a low temperature plasma of oxygen or oxygen plus an inert gas.
Claim 2
Dependent Claim
32 words
A carrier film for immobilizing protein comprising a polymeric film at least one surface of which has been incinerated using a low temperature plasma of oxygen or oxygen plus an inert gas.
Claim 3
Dependent Claim
13 words
A carrier film according to claim 1, wherein the polymeric film is porous.
Claim 4
Dependent Claim
20 words
A carrier film according to claim 2, wherein the pore size of the polymeric film is at least 250 A.
Claim 5
Dependent Claim
25 words
A carrier film according to any one of claims 1 to 3, wherein the polymeric film comprises carbon atoms, hydrogen atoms and optionally oxygen atoms.
Claim 6
Dependent Claim
20 words
A carrier film according to claim 4, wherein the polymeric film is a polyolefin, e.g. polypropylene, or a polycarbonate film.
Claim 7
Dependent Claim
37 words
6 . A carrier film according to any one of claims 1 to 5, wherein the thickness of the polymeric film before incineration is from 25 to 100 micrometers and after incineration is at least 15 micrometers.
Claim 8
Independent Claim
38 words
A method for producing a carrier film for immobilizing protein comprising treating a polymeric film with a low temperature plasma of oxygen or oxygen plus an inert gas to incinerate at least one surface of the polymeric film.
Claim 9
Independent Claim
38 words
A method for producing a carrier film for immobilizing protein comprising treating a polymeric film with a low temperature plasma of oxygen or oxygen plus an inert gas to incinerate at least one surface of the polymeric film.
Claim 10
Dependent Claim
17 words
A method according to claim 7, wherein the plasma is a low temperature plasma of pure oxygen.
Claim 11
Dependent Claim
25 words
A method according to claim 7 or claim 8, wherein the plasma treatment is carried out at a pressure of from 0.15 to 1 Torr.
Claim 12
Independent Claim
25 words
A method according to any one of claims 7 to 9, wherein the thickness of the polymeric film is reduced by at least 20 %.
Claim 13
Independent Claim
26 words
A method according to any one of claims 7 to 10 and further defined by the specific feature of any one of claims 2 to 5.
Claim 14
Dependent Claim
16 words
A protein immobilized on a carrier film according to any one of claims 1 to 6.
Claim 15
Dependent Claim
15 words
The use of a protein according to claim 12 in an analysis or assay technique.
References Cited (Backward Citations)
No citation records indexed.
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