EP0141382A2
Expired / Lapsed
EP
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Method for determining base sequence of DNA or DNA fragment utilizing autoradiography
Assignee: Fujifilm Holdings Corp
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Filing: 1984-10-25
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Granted: —
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Expires: 2004-10-25
Abstract
Abstract A method for determining the base sequence of DNA or DNA fragment utilizing autoradiography which comprises steps of: (1) preparing a mixture of radioactively labeled synthetic DNAs which are complementary to the DNA or DNA fragment to be sequenced; (2) resolving said mixture of synthetic DNAs on a support medium; (3) placing said support medium and a stimulable phosphor sheet in layers for a given period of time to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on the support medium, exciting said phosphor sheet with an electromagnetic wave to release the radiation energy stored in the phosphor sheet as stimulated emission, and detecting the stimulated emission, to obtain locational information on said radioactively labeled synthetic DNAs; and (4) determining the base sequence of said DNA or DNA fragment according to the obtained locational information.
Inventors
- 👤 Hisashi Shiraishi
- 👤 Junji C/O Fuji Photo Film Co. Ltd. Miyahara
- 👤 Hisatoyo C/O Fuji Photo Film Co. Ltd. Kato
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 14:45
CPC Classifications
G; G01; G01T; G01T1/00; G01T1/29; G01T1/2914; G01T1/2921; G01T1/2942; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6869
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
146 words
A method for determining the base sequence of DNA or DNA fragment utilizing autoradiography which comprises steps of: (1) preparing a mixture of radioactively labeled synthetic DNAs which are complementary to the DNA or DNA fragment to be sequenced; (2) resolving said mixture of synthetic DNAs on a support medium; (3) placing said support medium and a stimulable phosphor sheet in layers for a given period of time to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on the support medium, exciting said phosphor sheet with an electromagnetic wave to release the radiation energy stored in the phosphor sheet as stimulated emission, and detecting the stimulated emission, to obtain locational information on said radioactively labeled synthetic DNAs; and (4) determining the base sequence of said DNA or DNA fragment according to the obtained locational information.
Claim 2
Independent Claim
146 words
A method for determining the base sequence of DNA or DNA fragment utilizing autoradiography which comprises steps of: (1) preparing a mixture of radioactively labeled synthetic DNAs which are complementary to the DNA or DNA fragment to be sequenced; (2) resolving said mixture of synthetic DNAs on a support medium; (3) placing said support medium and a stimulable phosphor sheet in layers for a given period of time to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on the support medium, exciting said phosphor sheet with an electromagnetic wave to release the radiation energy stored in the phosphor sheet as stimulated emission, and detecting the stimulated emission, to obtain locational information on said radioactively labeled synthetic DNAs; and (4) determining the base sequence of said DNA or DNA fragment according to the obtained locational information.
Claim 3
Dependent Claim
33 words
The method for determining the base sequence of DNA or DNA fragment utilizing autoradiography as claimed in claim 1, wherein said synthetic DNAs in the step (1) are those prepared by Sanger-Coulson method.
Claim 4
Dependent Claim
35 words
The method for determining the base sequence of DNA or DNA fragment utilizing autoradiography as claimed in claim 2, wherein said synthetic DNAs in the step (1) are those prepared by the dideoxy terminating method.
Claim 5
Independent Claim
39 words
The method for determining the base sequence of DNA or DNA fragment utilizing autoradiography as claimed in any one of claims 1 to 3, wherein said mixture of synthetic DNAs in the step (2) is resolved by gel electrophoresis.
Claim 6
Independent Claim
43 words
The method for determining the base sequence of DNA or DNA fragment utilizing autoradiography as claimed in any one of claims 1 to 3, wherein said stimulable phosphor sheet in the step (3) is excited by sequentially scanning it with an electromagnetic wave.
Claim 7
Independent Claim
42 words
The method for determining the base sequence of DNA or DNA fragment utilizing autoradiography as claimed in any one of claims 1 to 3, wherein said locational information on the radioactively labeled substances in the step (3) is obtained as an image.
Claim 8
Independent Claim
46 words
The method for determining the base sequence of DNA or DNA fragment utilizing autoradiography as claimed in any one of claims 1 to 3, wherein said locational information on the radioactively labeled substances in the step (3) is obtained in the form of symbols and/or numerals.
Claim 9
Independent Claim
47 words
The method for determining the base sequence of DNA or DNA fragment utilizing autoradiography as claimed in any one of claims 1 to 3, wherein said stimulable phosphor sheet comprises a support, a phosphor layer comprising a stimulable phosphor dispersed in a binder, and a protective film.
References Cited (Backward Citations)
No citation records indexed.
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