EP0141298A1
Expired / Lapsed
EP
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Analytical method and element for albumin
Assignee: Eastman Kodak Co
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Filing: 1984-10-09
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Granted: —
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Expires: 2004-10-09
Abstract
Abstract An assay for the determination or detection of albumin in a liquid sample involves the use of an oxonol dye. This dye has a high affinity for albumin over a wide pH range. When the dye is bound to albumin, a shift in the dye spectral absorption occurs. The dye can be used in either wet or dry analytical methods.
Inventors
- 👤 Karl John Sanford
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 17:16
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6827; G01N33/6839
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
59 words
A method for the assay or detection of albumin in an aqueous liquid comprising the steps of: (1) physically contacting a sample of the liquid and a dye to produce a shift in the λ max , and (2) detecting the absorbance of that shifted λ max , the method characterized in that the dye is an oxonol dye.
Claim 2
Independent Claim
59 words
A method for the assay or detection of albumin in an aqueous liquid comprising the steps of: (1) physically contacting a sample of the liquid and a dye to produce a shift in the λ max , and (2) detecting the absorbance of that shifted λ max , the method characterized in that the dye is an oxonol dye.
Claim 3
Dependent Claim
35 words
The method as claimed in claim 1 in which the liquid sample and the dye are contacted in the presence of a buffer which maintains a pH in the range of from 5 to 11.
Claim 4
Independent Claim
19 words
The method as claimed in either of claims 1 or 2 in which the aqueous liquid is blood serum.
Claim 5
Dependent Claim
16 words
The use of an oxonol dye to detect or assay for albumin in an aqueous liquid.
Claim 6
Dependent Claim
16 words
The use of an oxonol dye to detect or assay for albumin in an aqueous liquid.
Claim 7
Dependent Claim
25 words
A dry analytical element for the assay or detection of albumin in a liquid sample, the element characterized in that it comprises an oxonol dye.
Claim 8
Dependent Claim
25 words
A dry analytical element for the assay or detection of albumin in a liquid sample, the element characterized in that it comprises an oxonol dye.
Claim 9
Dependent Claim
17 words
The element as claimed in claim 5 comprising a support and a reagent zone containing the dye.
Claim 10
Dependent Claim
16 words
The element as claimed in either of claims 5 or 6 comprising a porous spreading zone.
Claim 11
Dependent Claim
32 words
The element as claimed in any of claims 5 to 7 comprising a buffer which maintains the pH in the range of from 5 to 11 when contacted with the liquid sample.
Claim 12
Dependent Claim
30 words
The element as claimed in any of claims 5 to 8 in which the dye is present at a coverage of from 0.01 to 3 m g/ m 2 .
Claim 13
Dependent Claim
79 words
The invention as claimed in any of claims 1 to 9 in which the oxonol dye has the structure wherein n is zero or a positive integer up to 3, R 1 , R 2 and R 3 are independently hydrogen, alkyl, cycloalkyl, aryl, alkoxy, aryloxy, hydroxy, carboxy, alkoxycarbonyl, amino or a nonaromatic heterocyclic group, and Z and Zi independently represent the carbon, nitrogen, oxygen or sulfur atoms needed to complete a 5- to 7-membered carbocyclic or heterocyclic ring.
Claim 14
Dependent Claim
51 words
The invention as claimed in claim 10 in which n is 1 or 2, R 1 , R 2 and R 3 are independently hydrogen, alkyl or aryl, and Z and Z 1 independently represent the carbon, nitrogen, oxygen or sulfur atoms needed to complete a 5- or 6-membered heterocyclic ring.
Claim 15
Dependent Claim
16 words
The invention as claimed in any of claims 1 to 11 in which the dye is
Claim 16
Dependent Claim
16 words
The invention as claimed in any of claims 1 to 12 in which the dye is
References Cited (Backward Citations)
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