EP0140308A2
Expired / Lapsed
EP
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Regulation of gene expression by employing translational inhibition utilizing mRNA interfering complementary RNA
Assignee: Research Foundation of the State University of New York
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Filing: 1984-10-19
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Granted: —
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Expires: 2004-10-19
Abstract
Abstract Gene expression of the genetic material of cellular material or an organism is regulated or inhibited by incorporating in or along with the genetic material of the cellular material or organisms DNA or other genetic material which transcribes to an RNA which is complementary to and capable of binding to the mRNA of the genetic material of said organism. Gene expression or regulation of a gene is controlled by expressing a DNA fragment or replica of said gene inserted or positioned after a promoter in the opposite direction relative to the gene so as to regulate or inhibit the expression of said gene.
Inventors
- 👤 Masayori Inouye
- 👤 Takeshi Mizuno
- 👤 Mei-Yin Chou
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 03:23
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C07; C07H; C07H21/00; A; A61; A61K; A61K38/00; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
81 words
A method of regulating gene expression of an organism or cellular material which comprises incorporating in or associating with the genetic material of said organism or cellular material DNA which upon transcription along with the genetic material of said organism or cellular material produces an oligoribonucleotide complementary to and/or capable of binding or hybridizing with an mRNA produced by the genetic material of said organism or cellular material so that the expression or translation of said mRNA is inhibited or prevented.
Claim 2
Independent Claim
81 words
A method of regulating gene expression of an organism or cellular material which comprises incorporating in or associating with the genetic material of said organism or cellular material DNA which upon transcription along with the genetic material of said organism or cellular material produces an oligoribonucleotide complementary to and/or capable of binding or hybridizing with an mRNA produced by the genetic material of said organism or cellular material so that the expression or translation of said mRNA is inhibited or prevented.
Claim 3
Dependent Claim
21 words
2 . A method in accordance with Claim 1 wherein said organism or cellular material is Escherichia coli or Bacillus subtilis.
Claim 4
Dependent Claim
77 words
A transformed organism or cellular material wherein along with the genetic material of said organism or cellular material there is incorporated or associated therewith DNA which upon transcription along with the genetic material of said organism or cellular material produces an oligoribonucleotide complementary to and/or capable of binding or hybridizing with an mRNA produced by the genetic material of said organism or cellular material, so that the expression or translation of said mRNA is inhibited or prevented.
Claim 5
Dependent Claim
77 words
A transformed organism or cellular material wherein along with the genetic material of said organism or cellular material there is incorporated or associated therewith DNA which upon transcription along with the genetic material of said organism or cellular material produces an oligoribonucleotide complementary to and/or capable of binding or hybridizing with an mRNA produced by the genetic material of said organism or cellular material, so that the expression or translation of said mRNA is inhibited or prevented.
Claim 6
Dependent Claim
49 words
4 . A transformed' organism or cellular material in accordance with Claim 3 wherein said DNA is incorporated or associated with the genetic material of said organism or cellular material in the nucleus thereof or is present in a plasmid or is present in a virus or viral vector.
Claim 7
Dependent Claim
35 words
5 . A transformed organism or cellular material in accordance with Claim 3wherein said DNA is incorporated or associated with the genetic material of said organism or cellular material intracellularly or in the cytoplasm thereof.
Claim 8
Dependent Claim
48 words
6 . DNA material or molecule which upon transcription in a transformed-organism or cellular material contain-- ing said DNA-material or molecule produces an oligoribonucleotide or polyribonucleotide complementary to and/or capable of binding or hybridizing with an mRNA produced by the genetic material of said organism or cellular material.
Claim 9
Dependent Claim
48 words
6 . DNA material or molecule which upon transcription in a transformed-organism or cellular material contain-- ing said DNA-material or molecule produces an oligoribonucleotide or polyribonucleotide complementary to and/or capable of binding or hybridizing with an mRNA produced by the genetic material of said organism or cellular material.
Claim 10
Dependent Claim
44 words
DNA material or molecule in accordance with Claim 6 wherein said DNA material or molecule is incorporated in a plasmid or in a virus or viral vector or is incorporated in or associated with the chromosomal genetic material of said organism or cellular material.
Claim 11
Dependent Claim
32 words
DNA material or molecule-in accordance with Claim 6 wherein said DNA material or molecule is incorporated in or associated with the genetic material in the nucleus of said organism or cellular material.
Claim 12
Dependent Claim
37 words
A methcd of regulating the gene expression of a gene which comprises expressing a small DNA fragment or replica of said gene in the opposite direction so as to regulate or inhibit the expression of said gene.
Claim 13
Dependent Claim
37 words
A methcd of regulating the gene expression of a gene which comprises expressing a small DNA fragment or replica of said gene in the opposite direction so as to regulate or inhibit the expression of said gene.
Claim 14
Independent Claim
59 words
A method of regulating the genetic expression of an organism containing genetic material including a gene which is expressed therein which comprises inserting into said organism for expression therein a small DNA fragment or replica of said gene for expression therein in the opposite orientation so as to regulate or inhibit the expression of said gene in said organism.
Claim 15
Independent Claim
59 words
A method of regulating the genetic expression of an organism containing genetic material including a gene which is expressed therein which comprises inserting into said organism for expression therein a small DNA fragment or replica of said gene for expression therein in the opposite orientation so as to regulate or inhibit the expression of said gene in said organism.
Claim 16
Independent Claim
52 words
A method of regulating the gene expression of an oncogene in an organism which comprises inserting into said organism for expression therein a small DNA fragment or replica of said gene for expression therein in the opposite direction so as to regulate or inhibit the expression of said gene in said organism.
Claim 17
Independent Claim
52 words
A method of regulating the gene expression of an oncogene in an organism which comprises inserting into said organism for expression therein a small DNA fragment or replica of said gene for expression therein in the opposite direction so as to regulate or inhibit the expression of said gene in said organism.
Claim 18
Independent Claim
50 words
A method of regulating the gene expression of a viral gene in an organism which comprises inserting into said organism a small DNA fragment or replica of said gene for expression therein in the opposite direction so as to regulate or inhibit the expression of said gene in said organism.
Claim 19
Independent Claim
50 words
A method of regulating the gene expression of a viral gene in an organism which comprises inserting into said organism a small DNA fragment or replica of said gene for expression therein in the opposite direction so as to regulate or inhibit the expression of said gene in said organism.
Claim 20
Independent Claim
20 words
A method in accordance with Claims 9 or 1 0 wherein said small DNA fragment or replica includes a promoter.
Claim 21
Independent Claim
25 words
A method in accordance with claims 9, 10, 11 or 12 wherein said small DNA fragment or replica is inserted or provided after a promoter.
Claim 22
Independent Claim
32 words
A method in accordance with Claims 11 or 12 wherein said DNA fragment or replica is inserted into said organism via a vector or is inserted into said organism via a plasmid.
Claim 23
Dependent Claim
45 words
An organism having in its genetic make-up a gene capable of expression therein together with a small DNA fragment or replica of said gene inserted or positioned after a promoter in the opposite direction so as to regulate or inhibit the expression of said gene.
Claim 24
Dependent Claim
45 words
An organism having in its genetic make-up a gene capable of expression therein together with a small DNA fragment or replica of said gene inserted or positioned after a promoter in the opposite direction so as to regulate or inhibit the expression of said gene.
Claim 25
Dependent Claim
13 words
An organism in accordance with Claim 16 wherein said organism is Escherichia coli.
Claim 26
Dependent Claim
40 words
An organism in accordance with Claim 16 - wherein said DNA fragment or replica of said gene is inserted into said organism as part of its genetic make-up via a vector for example a virus vector or a plasmid vector.
Claim 27
Dependent Claim
76 words
An organism containing as part of its genetic make up a gene capable cf expression or transcription to produce its corresponding mRNA and containing also as part of its genetic make up a DNA fragment or replica of said gene and which is expressed or transcribed in the opposite direction relative to said gene so as to produce RNA complementary to and regulatory or inhibitory of said mRNA with respect to the translation of said mRNA.
Claim 28
Dependent Claim
76 words
An organism containing as part of its genetic make up a gene capable cf expression or transcription to produce its corresponding mRNA and containing also as part of its genetic make up a DNA fragment or replica of said gene and which is expressed or transcribed in the opposite direction relative to said gene so as to produce RNA complementary to and regulatory or inhibitory of said mRNA with respect to the translation of said mRNA.
Claim 29
Dependent Claim
88 words
A vector useful for controlling the expression or transcription of a gene in an organism, said vector comprising a DN A fragment or a replica of said gene which is capable of expression or transcription in said organism, said DNA fragment or replica of said gene being positioned after a promoter and in the opposite direction so that upon transcription when inserted in said organism, there is produced an RN A complementary to and regulatory or inhibitory with respect to the translation of the mRNA of said gene.
Claim 30
Dependent Claim
88 words
A vector useful for controlling the expression or transcription of a gene in an organism, said vector comprising a DN A fragment or a replica of said gene which is capable of expression or transcription in said organism, said DNA fragment or replica of said gene being positioned after a promoter and in the opposite direction so that upon transcription when inserted in said organism, there is produced an RN A complementary to and regulatory or inhibitory with respect to the translation of the mRNA of said gene.
Claim 31
Independent Claim
55 words
A method of protecting an organism against the toxic expression of a gene which may be introduced into said organism from an external source which comprises incorporating in said organism as part of the genetic make-up thereof a small DNA fragment or replica of said gene for expression therein in the opposite orientation or direction.
Claim 32
Independent Claim
55 words
A method of protecting an organism against the toxic expression of a gene which may be introduced into said organism from an external source which comprises incorporating in said organism as part of the genetic make-up thereof a small DNA fragment or replica of said gene for expression therein in the opposite orientation or direction.
References Cited (Backward Citations)
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