EP0139216A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Adult T cell leukemia virus antigen peptide
Assignee: Japanese Foundation for Cancer Research
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Filing: 1984-09-14
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Granted: —
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Expires: 2004-09-14
Abstract
Abstract Recombinant plasmids are constructed by inserting a DNA fragment coding for adult T cell leukemia virus antigen peptide into a vector DNA. Microorganisms are transformed with the recombinant plasmid and thereafter cultured to express the peptide. The adult T cell leukemia virus antigen peptide is useful for the detection and diagnosis of adult T cell leukemia.
Inventors
- 👤 Tadatsugu Taniguchi
- 👤 Mitsuaki Yoshida
- 👤 Haruo Sugano
- 👤 Susumu Sekine
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 02:37
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; A; A61; A61K; A61K38/00; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/14011; C12N2740/14022
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
7 words
Adult T cell leukemia virus antigen peptide.
Claim 2
Dependent Claim
7 words
Adult T cell leukemia virus antigen peptide.
Claim 3
Dependent Claim
13 words
A peptide according to claim 1, which is encoded by the gag gene.
Claim 4
Dependent Claim
17 words
A peptide according to claim 2, which is encoded by the p24 region of the gag gene.
Claim 5
Dependent Claim
22 words
A recombinant plasmid constructed by inserting a DNA fragment coding for adult T cell leukemia virus antigen peptide in a vector DNA.
Claim 6
Dependent Claim
22 words
A recombinant plasmid constructed by inserting a DNA fragment coding for adult T cell leukemia virus antigen peptide in a vector DNA.
Claim 7
Dependent Claim
67 words
A recombinant plasmid according to claim 4, characterized in that the DNA fragment coding for adult T cell leukemia virus antigen peptide is operatively linked to an expression control sequence selected from the group of the E. coli λ promoter system, the E. coli lac system, the E. coli β-lactamase system, the E. coli trp-system, the E. coli lipoprotein promoter, yeast and other eukaryotic expression control sequences.
Claim 8
Dependent Claim
33 words
6 . A recombinant plasmid according to claim 4, wherein a tryptophan promoter or a lactose promoter is positioned upstream from said DNA fragment coding for adult T cell leuKemia virus antigen peptide.
Claim 9
Dependent Claim
13 words
A recombinant plasmid according to claim 6, which is plasmid pTAG424A or pTAG424B.
Claim 10
Independent Claim
61 words
A method of producing adult T cell leukemia virus antigen peptide, which comprises culturing in a nutrient medium a microorganism harboring a recombinant plasmid constructed by inserting a DNA fragment coding for adult T cell leukemia virus antigen peptide in a vector plasmid, accumulating adult T cell leukemia virus antigen peptide in the culture - and recovering the said peptide tnerefrom.
Claim 11
Independent Claim
61 words
A method of producing adult T cell leukemia virus antigen peptide, which comprises culturing in a nutrient medium a microorganism harboring a recombinant plasmid constructed by inserting a DNA fragment coding for adult T cell leukemia virus antigen peptide in a vector plasmid, accumulating adult T cell leukemia virus antigen peptide in the culture - and recovering the said peptide tnerefrom.
Claim 12
Dependent Claim
67 words
A method according to claim 8, wherein the recombinant plasmid contains said DNA fragment coding for adult T cell leukemia virus antigen peptide operatively linked to an expression control sequence selected from the group of the E. coli λpromoter system, the E. coli lac system, the E . coli f3-lactamase system, the E. coli trp-system, the E. coli lipoprotein promoter, yeast and other eukaryotic expression control sequences.
Claim 13
Dependent Claim
32 words
A method according to claim 8, wherein the recombinant plasmid includes a tryptophan promoter or a lactose promoter upstream from said DNA fragment coding for adult T cell leukemia virus antigen peptide.
Claim 14
Dependent Claim
16 words
1'1. A method according to claim 8 , wherein said microorganism belongs to the genus Escherichia.
Claim 15
Dependent Claim
17 words
12 . A method according to claim 11, wherein said microorganism is a strain of Escherichia coli.
Claim 16
Dependent Claim
14 words
A method according to claim 10, wherein said recombinant plasmid is pTAG424A or pTAG424B.
Claim 17
Dependent Claim
26 words
A microorganism which harbors a recombinant plasmid constructed by inserting a DNA fragment coding for adult T cell leukemia virus antigen peptide in a vector DNA.
Claim 18
Dependent Claim
26 words
A microorganism which harbors a recombinant plasmid constructed by inserting a DNA fragment coding for adult T cell leukemia virus antigen peptide in a vector DNA.
Claim 19
Dependent Claim
69 words
A microorganism according to claim 14, wherein the recombinant plasmid contains said DN A fragment coding for adult T cell leukemia virus antigen peptide operatively linked to an expression control sequence selected from the group of the E. coli λ promoter system, the E . coli lac system, the E. coli β-lactamase system, the E. coli trp-system, the E. coli lipoprotein promoter, yeast and other eukaryotic expression control sequences.
Claim 20
Dependent Claim
28 words
A microorganism according to claim 14., wherein said recombinant plasmid includes a tryptophan promoter upstream from said DNA fragment coding for adult T cell leukemia virus antigen peptide.
Claim 21
Dependent Claim
28 words
A microorganism according to claim 14, wherein said recombinant plasmid includes a lactose promoter upstream from said DNA fragment coding for adult T cell leukemia virus antigen peptide.
Claim 22
Dependent Claim
12 words
A microorganism according to claim 14, which is -a-strain of Escherichia coli.
References Cited (Backward Citations)
No citation records indexed.
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