EP0138092A2
Expired / Lapsed
EP
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Method of amino acid analysis
Assignee: Shimadzu Corp
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Filing: 1984-09-20
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Granted: —
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Expires: 2004-09-20
Abstract
Abstract A method of amino acid analysis by reacting an amino acid-containing sample with o-phthalaldehyde and an N-protective group-substituted cysteine such as N-acetylcysteine under an alkaline condition to yield a fluorescent compound and measuring the intensity of fluorescence or absorbance of the resulting compound, which is sensitive for quantitative and qualitative analysis of a secondary amino acid such as proline when it is beforehad subjected to an oxidation.
Inventors
- 👤 Yasuo Ishida
- 👤 Michihiko 2-501 Fuei Jyutaku 69 Fujiwara
- 👤 Toshio Kinoshita
- 👤 Noriyuki Nimura
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 15:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6806; G01N33/6812; G01N33/6815; G01N33/6809
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
42 words
A method of amino acid analysis which comprises reacting an amino acid-containing sample with o-phthalaldehyde and an N-protective group-substituted cysteine under an alkaline , condition to yield a fluorescent compound and measuring the intensity of fluorescence or absorbance of the resulting compound.
Claim 2
Independent Claim
42 words
A method of amino acid analysis which comprises reacting an amino acid-containing sample with o-phthalaldehyde and an N-protective group-substituted cysteine under an alkaline , condition to yield a fluorescent compound and measuring the intensity of fluorescence or absorbance of the resulting compound.
Claim 3
Dependent Claim
18 words
The method of claim 1 in which the amino acid-containing sample is one containing a primary amino acid.
Claim 4
Dependent Claim
27 words
The method of claim 1 in which the amino acid-containing sample is a secondary amino acid-containing sample which is used to the analysis after an oxidation treatment.
Claim 5
Dependent Claim
20 words
The method of claim 3 in which the secondary amino acid-containing sample is one containing proline and/or N-lower alkyl-substituted glycine.
Claim 6
Dependent Claim
22 words
The method of claim 1 in which the reaction is conducted in an alkaline buffer having a pH of 9 to 12.
Claim 7
Dependent Claim
20 words
The method of claim 5 in which the alkaline buffer is a borate type, carbonate type or borate-carbonate type buffer.
Claim 8
Dependent Claim
28 words
The method of claim 1 in which a protective group in the N-protective group-substituted cystein is acetyl, trifluoroacetyl, ethoxycarbonyl, ter-butoxycarbonyl, benzyloxycarbonyl, p-nitrobenzyloxycarbonyl, p-nitrophenyl- sulfonyl, p-toluenesulfonyl or trityl group.
Claim 9
Dependent Claim
13 words
The method of claim 1 in which the N-protective group-substituted cystein is N-acetyl-L-cystein.
Claim 10
Dependent Claim
20 words
The method of claim 1 in which the amino acid-containing sample is before the analysis subjected to a liquid chromatography.
Claim 11
Dependent Claim
20 words
The method of claim 1 in which the resulting fluorescent compound is before the measurement subjected to a liquid. chromatography,
Claim 12
Dependent Claim
28 words
The method of claim 1 in which the amino acid-containing sample contains D,L-amino acid and the resulting fluorescent diastereomers are before the measurement subjected to an optical resolution.
Claim 13
Dependent Claim
72 words
An amino acid analyzer comprising a buffer channel extending from a feeder for amino acid-containing samples and connected to a separating column, an eluate channel extending from the separating column and connected to a detector having means for measuring the intensity of fluorescence or absorbance, and a mixture reactor provided for the buffer channel or the eluate channel for accommodating an alkaline buffer solution of o-phthalaldehyde and an N- protective group-substituted cystein.
Claim 14
Dependent Claim
72 words
An amino acid analyzer comprising a buffer channel extending from a feeder for amino acid-containing samples and connected to a separating column, an eluate channel extending from the separating column and connected to a detector having means for measuring the intensity of fluorescence or absorbance, and a mixture reactor provided for the buffer channel or the eluate channel for accommodating an alkaline buffer solution of o-phthalaldehyde and an N- protective group-substituted cystein.
Claim 15
Dependent Claim
20 words
The amino acid analyzer of claim 12 further compring a pre-reactor for hypochlorous acid disposed upstream from the mixture reactor.
References Cited (Backward Citations)
No citation records indexed.
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